Larval exposure to thiamethoxam and American foulbrood: effects on mortality and cognition in the honey bee <i>Apis mellifera</i>

Papach, Anna; Fortini, Dominique; Grateau, Stéphane; Aupinel, Pierrick; Richard, Freddie-Jeanne

doi:10.1080/00218839.2017.1332541

Cited by 21 publications

(22 citation statements)

References 47 publications

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“…This is remarkable given that there was a time span of four weeks between the pesticide exposure in larvae and the measures of gene expression in adults, which is almost spanning the total life expectancy of honeybee workers 54 . This indicates that bees that are exposed early in life to a pesticide are substantially more susceptible to environmental stress once they start foraging 55,56 , which could have important ramifications on the colony level if this results in a reduction in total foraging time or efficiency. Future work is therefore required to quantify the effects of pesticide exposure on foraging efficiency and length, for example using already established methods of tracking bees with radio frequency tags in the field 57,58 .…”

Section: Discussionmentioning

confidence: 99%

Consequences of a short time exposure to a sublethal dose of Flupyradifurone (Sivanto) pesticide early in life on survival and immunity in the honeybee (Apis mellifera)

Naggar

Baer

2019

Sci Rep

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Dramatic losses of pollinating insects have become of global concern, as they threaten not only key ecosystem services but also human food production. Recent research provided evidence that interactions between ecological stressors are drivers of declining pollinator health and responsible for observed population collapses. We used the honeybee Apis mellifera and conducted a series of experiments to test for long-term effects of a single short exposure to the agricultural pesticide flupyradifurone to a second environmental stressor later in life. To do this, we exposed individuals during their larval development or early adulthood to sublethal dosages of flupyradifurone (0.025 μg for larvae and 0.645 μg for imagos), either pure or as part of an agricultural formulation (Sivanto). We afterwards exposed bees to a second ecological stressor infecting individuals with 10,000 spores of the fungal gut parasite Nosema ceranae. We found that pesticide exposures significantly reduced survival of bees and altered the expression of several immune and detoxification genes. The ability of bees to respond to these latter effects differed significantly between colonies, offering opportunities to breed bees with elevated levels of pesticide tolerance in the future. We conclude that short episodes of sublethal pesticide exposures during development are sufficient to trigger effects later in life and could therefore contribute to the widespread declines in bee health.

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Section: Discussionmentioning

confidence: 99%

Consequences of a short time exposure to a sublethal dose of Flupyradifurone (Sivanto) pesticide early in life on survival and immunity in the honeybee (Apis mellifera)

Naggar

Baer

2019

Sci Rep

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“…Papach et al ( 2017 ) present the first evidence of impaired learning and memory in adult bees that were fed thiamethoxam (0.6 ng/bee) during the larval stage. Colony survival critically depends on successful learning and memory.…”

Section: Part A: Invertebratesmentioning

confidence: 99%

An update of the Worldwide Integrated Assessment (WIA) on systemic insecticides. Part 2: impacts on organisms and ecosystems

Pisa

Goulson

Yang

et al. 2017

Environ Sci Pollut Res

190

144

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New information on the lethal and sublethal effects of neonicotinoids and fipronil on organisms is presented in this review, complementing the previous Worldwide Integrated Assessment (WIA) in 2015. The high toxicity of these systemic insecticides to invertebrates has been confirmed and expanded to include more species and compounds. Most of the recent research has focused on bees and the sublethal and ecological impacts these insecticides have on pollinators. Toxic effects on other invertebrate taxa also covered predatory and parasitoid natural enemies and aquatic arthropods. Little new information has been gathered on soil organisms. The impact on marine and coastal ecosystems is still largely uncharted. The chronic lethality of neonicotinoids to insects and crustaceans, and the strengthened evidence that these chemicals also impair the immune system and reproduction, highlights the dangers of this particular insecticidal class (neonicotinoids and fipronil), with the potential to greatly decrease populations of arthropods in both terrestrial and aquatic environments. Sublethal effects on fish, reptiles, frogs, birds, and mammals are also reported, showing a better understanding of the mechanisms of toxicity of these insecticides in vertebrates and their deleterious impacts on growth, reproduction, and neurobehaviour of most of the species tested. This review concludes with a summary of impacts on the ecosystem services and functioning, particularly on pollination, soil biota, and aquatic invertebrate communities, thus reinforcing the previous WIA conclusions .

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“…In addition, a few other studies have focused on the interaction between the sublethal exposure to neonicotinoids and other pathogens. For example, the combination of the fungus Nosema ceranae and imidacloprid resulted in increased mortality in bees [45], the combination of the bacterium Paenibacillus larvae and thiamethoxam resulted in increased mortality and decreased learning and memory [46], and the combination of black queen cell virus (BQCV) and clothianidin decreased larval survival [47]. These results indicate that there are detrimental effects on the health of honey bees when parasites and pathogens are combined with sublethal exposures to neonicotinoid insecticides, but the interaction can have different outcomes, depending on the biological agent under study, the dose and type of neonicotinoid insecticide, and the aspects of honey bee health measured.…”

Section: Introductionmentioning

confidence: 99%

Interaction of field realistic doses of clothianidin and Varroa destructor parasitism on adult honey bee (Apis mellifera L.) health and neural gene expression, and antagonistic effects on differentially expressed genes

Morfin

Guzmán-Novoa

2020

PLoS ONE

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While many studies have examined the effects of neonicotinoid insecticides and the parasitic mite Varroa destructor on honey bees (Apis mellifera), more information on the combined effects of such stressors on gene expression, including neural related genes, and their impact on biological pathways is needed. This study analyzed the effects of field realistic concentrations of the neonicotinoid clothianidin on adult bees infested and not infested with V. destructor over 21 consecutive days and then determined bee survivorship, weight, deformed wing virus (DWV) levels and gene expression. V. destructor parasitism with or without clothianidin exposure was significantly associated with decreased survivorship, weight loss and higher DWV levels, while clothianidin exposure was only associated with higher levels of DWV. Expression analysis of the neural genes AmNlg-1, BlCh and AmAChE-2 showed that V. destructor caused a significant down-regulation of all of them, whereas clothianidin caused a significant down-regulation of only AmNrx-1 and BlCh. An interaction was only detected for AmNrx-1 expression. RNAseq analysis showed that clothianidin exposure resulted in 6.5 times more up-regulated differentially expressed genes (DEGs) than V. destructor alone and 123 times more than clothianidin combined with V. destructor. Similar results were obtained with down-regulated DEGs, except for a higher number of DEGs shared between V. destructor and the combined stressors. KEGG (Kyoto Encyclopedia of Genes and Genomes) biological pathway analysis of the DEGs showed that the stressor linked to the highest number of KEGG pathways was clothianidin, followed by V. destructor, and then considerably fewer number of KEGG pathways with the combined stressors. The reduced numbers of DEGs and KEGG pathways associated with the DEGs for the combined stressors compared to the stressors alone indicates that the interaction of the stressors is not additive or synergistic, but antagonistic. The possible implications of the antagonistic effect on the number of DEGs are discussed.

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Larval exposure to thiamethoxam and American foulbrood: effects on mortality and cognition in the honey bee Apis mellifera

Cited by 21 publications

References 47 publications

Consequences of a short time exposure to a sublethal dose of Flupyradifurone (Sivanto) pesticide early in life on survival and immunity in the honeybee (Apis mellifera)

Consequences of a short time exposure to a sublethal dose of Flupyradifurone (Sivanto) pesticide early in life on survival and immunity in the honeybee (Apis mellifera)

An update of the Worldwide Integrated Assessment (WIA) on systemic insecticides. Part 2: impacts on organisms and ecosystems

Interaction of field realistic doses of clothianidin and Varroa destructor parasitism on adult honey bee (Apis mellifera L.) health and neural gene expression, and antagonistic effects on differentially expressed genes

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