Larval development of Spodoptera eridania (Cramer) fed on leaves of Bt maize expressing Cry1F and Cry1F + Cry1A.105 + Cry2Ab2 proteins and its non-Bt isoline

Bortolotto, Orcial Ceolin; Bueno, Adeney de Freitas; Queiroz, Ana Paula de; Silva, Gabriela Vieira; Barbosa, Gustavo Caselato

doi:10.1016/j.rbe.2014.12.001

Cited by 4 publications

(6 citation statements)

References 23 publications

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“…However, this number on the control treatment of Cry2Ab2 was less than that on the toxins treated insects by 0.21 to 0.27. In general, these results were consistent with the findings of Bortolotto et al (2015) with toxin Cry1F and Cry1F + Cry1A.105 + Cry2Ab2 can reduce larval growth of S. eridania, although this larva was not their insect target. According to Puspita (2015) Cry1A.105 sublethal concentration can decrease the rate of growth and delay the development of O. furnacalis larvae into the pupal and adult stages.…”

Section: The Effect Of Bt Toxin Cry2ab2 On Spodoptera Liturasupporting

confidence: 90%

THE EFFECT OF Bacillus thuringiensis TOXIN Cry1A.105 AND Cry2Ab2 ON THE SURVIVAL OF THE NON-TARGET PEST, Spodoptera litura (PENGARUH TOKSIN Bacillus thuringiensis Cry1A.105 DAN Cry2Ab2 TERHADAP KELANGSUNGAN HIDUP HAMA BUKAN SASARAN, Spodoptera litura)

Pratiwi

Trisyono

Martono

2017

JPTI

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Spodoptera litura is one of the important insect pest of maize besides the notoriously damaging corn borer, Ostrinia furnacalis. S. litura has been the target of various controls including the use of Bacillus thuringiensis (Bt) toxin Cry1A.105 and Cry2Ab2. This study was conducted to evaluate the acute effect of Bt toxin Cry1A.105 and Cry2Ab2 on the growth and development of S. litura from larval to adult stages. Two sublethal concentrations were used; 0.1875 and 0.0469 ppm for Cry1A.105, and 0.0008 and 0.0003 ppm for Cry2Ab2. The bioassay using diet dipping was carried out on a CRD with three experiments and five repetitions. The observation was carried out on the mortality and survival rates of S. litura. The mortality reached 28% when the larvae were treated with 0.1875 ppm and 20% with 0.0469 ppm of Cry 1A.105. The exposed larvae and pupae were smaller than control. Larval and pupal weight were 117.0 and 165.6 g with 0.1875 ppm, while control were 212.9 and 211.2 g. Cry1A.105 also longer the larval stage, larval stage with higher and lower concentration were 24.5 and 22.3 day, while control was 20.5 day. The resulted pupae from larve which exposed by Cry1A.105 were less than control; there were 40% at concentration 0.1875 ppm and control 61%. The two concentration of Cry2Ab2 produced similar mortality of 20%. Similarly, Cry2Ab2 affected pupal to adult stages development. The longevity of pupal stage with concentration 0.0003 ppm was 9.5 days, followed by 0.0008 ppm (9.1 days) and control (10.1 days). The adult emerge on the highest concentration was 47.4% while control only 34.6%. There results showed that both Cry1A.105 and Cry2Ab2 were detrimental to the survival of S. litura which is the non-target insect of transgenic Bt maize.

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Section: The Effect Of Bt Toxin Cry2ab2 On Spodoptera Liturasupporting

confidence: 90%

THE EFFECT OF Bacillus thuringiensis TOXIN Cry1A.105 AND Cry2Ab2 ON THE SURVIVAL OF THE NON-TARGET PEST, Spodoptera litura (PENGARUH TOKSIN Bacillus thuringiensis Cry1A.105 DAN Cry2Ab2 TERHADAP KELANGSUNGAN HIDUP HAMA BUKAN SASARAN, Spodoptera litura)

Pratiwi

Trisyono

Martono

2017

JPTI

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“…The Cry1F-Cry1A105-Cry2Ab2 pyramidal event caused a reduction of 55.14% in the leaf consumption of Spodoptera eridania (Cramer) (Lepidoptera: Noctuidae), in relation to the control group (Bortolotto et al, 2015), results higher than those found in this study, where maize with two proteins (Cry1A105-Cry2Ab2) had a reduction of 32.93% in the consumed leaf area. A low response in the conversion of ingested and deferred food by the insects may result in a greater consumption of leaf area; however, further studies are necessary in order to consolidate this premise.…”

Section: /8contrasting

confidence: 79%

“…A low response in the conversion of ingested and deferred food by the insects may result in a greater consumption of leaf area; however, further studies are necessary in order to consolidate this premise. The results contrast with the literature premises, that pyramidal events present a higher percentage of control, resistance prevention and lower leaf consumption (Bortolotto et al, 2015). Costa et al (2006), observed a 28.92 cm 2 .day -1 consumption of S. frugiperda in leaves of conventional maize, compatible with the result found in this study.…”

Section: /8contrasting

confidence: 56%

Survival and nutritional indexes of Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) maintained in Bt maize for five generations

Silva

Maggioni

Ferreira

et al. 2019

Agraria

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The selection pressure generated by the incorrect use of Bt maize might result in Spodoptera frugiperda individuals resistant to the toxins synthesized by the plant. In this study was evaluated the nutritional parameters of Spodoptera frugiperda submitted to the maize that synthesize the toxins Cry1F, Cry1A105-Cry2Ab2 and Vip3Aa20, during five subsequent generations of selection pressure. The caterpillars were submitted to the treatments: Non-Bt maize (CONV), Cry1F (Bt1), Cry1A105/Cry2Ab (Bt2) and Vip3Aa20 (VIP1, VIP2 and VIP3) and exposed for a period of four days in each cycle, for five successive generations. The larval survival of S. frugiperda was evaluated in five generations. The consumed leaf area was quantified in the second generation and the nutritional indexes in the latter ones. The results indicate that caterpillars of S. frugiperda from VIPs lineage presented the lowest percentage of survival, with a high Metabolic Cost and a considerable efficiency reduction in the conversion of the digested and ingested food. The lineage maintained in VIP2 showed the lowest leaf consumption (83.13%). However, the action of Vip3Aa20 toxin on S. frugiperda resulted in a higher Metabolic Cost in reduction of leaf area consumption. However, from the third generation on there is a survival of insects exposed to Bt toxins, suggesting the surviving potential of this species in Bt maize subjected to continuous exposure to this technology. Sobrevivência e índices nutricionais de Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) mantida em milho Bt por cinco gerações RESUMO: A pressão de seleção gerada pelo uso incorreto do milho Bt pode resultar em indivíduos de Spodoptera frugiperda resistentes às toxinas expressadas pela planta. Neste trabalho foi determinado os parâmetros nutricionais de Spodoptera frugiperda submetida aos milhos que sintetizam as toxinas Cry1F, Cry1A105-Cry2Ab2 e Vip3Aa20, durante cinco gerações. As lagartas foram submetidas aos tratamentos: Milho não-Bt (CONV), Cry1F (Bt1), Cry1A105/Cry2Ab (Bt2) e Vip3Aa20 (VIP1, VIP2 e VIP3) e expostas por um período de quatro dias em cada ciclo, por cinco gerações sucessivas. Foi avaliada a sobrevivência larval de S. frugiperda em cinco gerações. A área foliar consumida foi quantificada na segunda geração e nas posteriores foram determinados os índices nutricionais. As lagartas de S. frugiperda, da geração mantida no milho VIPs, apresentaram menor porcentagem de sobrevivência, com um alto Custo Metabólico e considerável redução na Eficiência na conversão do alimento digerido e Eficiência na conversão do alimento ingerido. A geração mantida no Hibrido VIP2 apresentou o menor consumo foliar (83,13%). Contudo a ação da toxina Vip3Aa20 sobre S. frugiperda resultou no maior Custo Metabólico em redução no consumo de área foliar. Contudo a partir da terceira geração observa-se uma sobrevivência de insetos expostos as toxinas Bt, sugerindo o potencial desta espécie em sobreviver aos milhos Bt quando submetidas a exposição contínua a esta tecnologia. Palavra...

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“…In recent years, a population increase of S. cosmioides rstly (Walker), and more recently, of S. eridania (Stoll) has been recorded in the central soybean planted area of Argentina, even in Bt cultivars (Lutz et al, 2015;Lutz et al, 2019). It is known that both species are tolerant to the Cry1Ac protein, and resistant to several insecticides (Luttrell et al, 1999;Bernardi et al, 2014;Montezano et al, 2014;Bortolotto et al, 2015;Machado et al, 2020). Spodoptera eridania has a development threshold at low temperatures, it does not complete its cycle above 34°C, hence it is favored in temperate regions, such as the Argentinian Pampas; meanwhile S. cosmioides adapts to warm temperatures, which is the reason why it is found in soybean and cotton crops in Northern Argentinian regions (Parra et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Impact of transgenic Bt soybean (Cry1Ac) on the biological and reproductive cycle and the hervibory ability of Spodoptera cosmioides (Walker) and Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae)

Lutz

Fernández

Ruiz

et al. 2023

Preprint

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The Spodoptera cosmioides (Walker) and Spodoptera eridania (Stoll) populations have increased in the central soybean crop zone of Argentina during the last years. Besides being polyphagous, these species are tolerant to the insecticidal toxin Cry1Ac, which is expressed by genetically modified Bt soybeans. The aim of this study was to determine the effect of Bt soybeans on the consumption, biological cycle and reproduction of both Spodoptera species. The Cry1Ac protein had not consequences in the biological and reproductive cycle of S. cosmioides, except in the pupal period duration. It was not observed the same response in S. eridania, which recorded a shorter duration of the larval and adult stages, total cycle and oviposition period. However, both species of Lepidoptera completed their reproductive cycle successfully and the herbivorous ability was not altered. The genetically modified soybean with the event MON89788xMON87701 that expresses the Cry1Ac protein does not control S. cosmioides and S. eridania, and farmers must appeal to chemical control with insecticides when high densities occur in the field. The results obtained in this work are very useful to understand the biology of these species, which have the potential to become important pests in Bt soybean crops in Argentina.

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Larval development of Spodoptera eridania (Cramer) fed on leaves of Bt maize expressing Cry1F and Cry1F + Cry1A.105 + Cry2Ab2 proteins and its non-Bt isoline

Cited by 4 publications

References 23 publications

THE EFFECT OF Bacillus thuringiensis TOXIN Cry1A.105 AND Cry2Ab2 ON THE SURVIVAL OF THE NON-TARGET PEST, Spodoptera litura (PENGARUH TOKSIN Bacillus thuringiensis Cry1A.105 DAN Cry2Ab2 TERHADAP KELANGSUNGAN HIDUP HAMA BUKAN SASARAN, Spodoptera litura)

THE EFFECT OF Bacillus thuringiensis TOXIN Cry1A.105 AND Cry2Ab2 ON THE SURVIVAL OF THE NON-TARGET PEST, Spodoptera litura (PENGARUH TOKSIN Bacillus thuringiensis Cry1A.105 DAN Cry2Ab2 TERHADAP KELANGSUNGAN HIDUP HAMA BUKAN SASARAN, Spodoptera litura)

Survival and nutritional indexes of Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) maintained in Bt maize for five generations

Impact of transgenic Bt soybean (Cry1Ac) on the biological and reproductive cycle and the hervibory ability of Spodoptera cosmioides (Walker) and Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae)

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