Larval anisakids parasitizing the blue whiting, <i>Micromesistius poutassou</i>, from Motril Bay in the Mediterranean region of southern Spain

Valero, A.; Martı́n-Sánchez, Joaquina; Reyes-Muelas, E; Adroher, Francisco Javier

doi:10.1017/s0022149x00000536

Cited by 50 publications

(32 citation statements)

References 16 publications

(24 reference statements)

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“…2). This tendency in the prevalence has also been reported for other fish species and, in many cases, was also statistically (Takao 1990;Adroher et al 1996;Hemmingsen et al 2000;Valero et al 2000Valero et al , 2006a. The low prevalence of the parasite in the youngest sardines may be due to the fish larvae, at least in the Gulf of Lyon, feeding preferentially on phytoplankton (Rasoanarivo et al 1991;Palomera et al 2007 for references), and, consequently, the youngest fish sent to market have had less opportunity than the longer ones to become infected.…”

Section: Resultssupporting

confidence: 66%

Hysterothylacium aduncum, the only anisakid parasite of sardines (Sardina pilchardus) from the southern and eastern coasts of Spain

Rello¹,

Adroher

Valero

2008

Parasitol Res

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An epidemiological study was carried out on the anisakids in sardines (Sardina pilchardus) from the southern (Atlantic and Mediterranean) and eastern coasts of Spain. Length of fish was from 12.2-21.0 cm. The anisakids found were identified as the third larval stage (L3) of Hysterothylacium aduncum, with a total prevalence of 11.85%. Prevalence within the host was 9.64% in viscera and 4.69 % in muscle. The highest infection parameters were found in fish from the east coast (western Balearic Sea) with prevalence of 25.21%, mean intensity of 2.10, and mean abundance of 0.52. No worms of the genus Anisakis were found in the 359 sardines analyzed.

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Section: Resultssupporting

confidence: 66%

Hysterothylacium aduncum, the only anisakid parasite of sardines (Sardina pilchardus) from the southern and eastern coasts of Spain

Rello¹,

Adroher

Valero

2008

Parasitol Res

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“…1, p<0.04). Several studies confirm this relationship between length and parasitization in other hosts (Takao 1990;Hemmingsen et al 2000;Valero et al 2000Valero et al , 2006.…”

Section: Discussionmentioning

confidence: 66%

“…These differences may be due to the different geographical origins of the fish. The infection parameters of different fish hosts vary from one geographical zone to another (Adroher et al 1996, Valero et al 2000, Rello 2003. According to Angot and Brasseur (1995), these fluctuations are the result of diverse biological factors such as: (a) the level of infection of marine mammal final hosts; (b) the abundance of obligatory intermediate hosts; and (c) the importance of each fish stock in the trophic chain since it includes all natural hosts which host the stages of the life cycle of the parasites and the trophic relationship among the all hosts.…”

Section: Discussionmentioning

confidence: 99%

Anisakis spp. in European hake, Merluccius merluccius (L.) from the Atlantic off north-west Africa and the Mediterranean off southern Spain

Valero

López-Cuello

Benı́tez

et al. 2006

Acta Parasitologica

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We studied the parasitization by Anisakis spp. in European hake (Merluccius merluccius) of 40-52 cm from the Atlantic off north-west Africa and the Mediterranean off southern Spain. Infection parameters differed: The fish from the Atlantic showing a prevalence of 87.97% and a mean intensity of 4.69, while, in those from the Mediterranean, these were 41.27% and 1.73, respectively. In both samples the two-third larval stage types were isolated: Anisakis simplex sensu lato and Anisakis larvae type II, with prevalence of 85.71% and 30.83% in fish from the Atlantic and 41.27% and 1.59% for those from the Mediterranean, respectively. In both samples, the prevalence of larvae in viscera was clearly higher than in the muscle tissue. We also observed an increase in parasitization with increasing host length, those $46 cm having the highest prevalences (94.87% for those from the Atlantic and 58.33% for those from the Mediterranean; p<0.03).

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“…Blue whiting, on the Atlantic and Mediterranean Spanish coasts, are frequently parasitized by H. aduncum (Ruiz-Valero et al 1992, Valero et al 2000. The worms, found free in the host body cavity, were collected with the help of a needle with a blunt tip, placed on a Petri dish and washed in 0.9% NaCl solution several times.…”

Section: Methodsmentioning

confidence: 99%

In vitro development of the fish parasite Hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg

Adroher¹,

Malagón²,

Valero³

et al. 2004

Dis. Aquat. Org.

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Anisakids are parasitic nematodes of fish worldwide, producing economic and human health concerns. It is thus important to ascertain their in vitro life cycle in laboratory studies. Here we describe the in vitro development of third-stage larvae (L3) of Hysterothylacium aduncum isolated from blue whiting Micromesistius poutassou, to the hatching of L3 from eggs obtained from H. aduncum worms grown in GLIT medium (a modified mixture of Yaeger's LIT [Liver Infusion Tryptose] and Grace's media) at pH 4.0, 13°C and with 5% CO 2 in air. Under these conditions, L3 recovered from fish developed to mature adults (3.4 to 6.2 cm in length), with oviposition starting from Day 26 in culture. Fertilized eggs (mean size 64 × 52 µm) had a thick, rugose eggshell and were larger than unfertilized ones (mean size 49 × 42 µm), whose eggshells were thin and smooth. Eggs laid during the first and second week of oviposition, and maintained in 2.8% NaCl solution at 13°C, developed to L3. Under these maintenance conditions, between 20.6 and 52.5% of the eggs laid during the first week developed into larvae. Motile larvae, enclosed in a sheath, hatched from between 2 and 11% of these eggs. The larvae started to hatch 23 d after deposition. These larvae were 144 to 215 µm in length, enclosed in a 237 to 305 µm-long sheath. This GLIT culture medium may help to study the biology of this and other anisakids.KEY WORDS: Fish parasite · Hysterothylacium aduncum · Nematoda · Anisakidae · In vitro culture · Egg development · Hatching Resale or republication not permitted without written consent of the publisherDis Aquat Org 58: [41][42][43][44][45] 2004 mesistius poutassou (blue whiting), family Gadidae, purchased from the fish market of Granada (southern Spain). Blue whiting, on the Atlantic and Mediterranean Spanish coasts, are frequently parasitized by H. aduncum (Ruiz-Valero et al. 1992, Valero et al. 2000. The worms, found free in the host body cavity, were collected with the help of a needle with a blunt tip, placed on a Petri dish and washed in 0.9% NaCl solution several times. The worms were observed individually under an inverted microscope, and those that showed any kind of internal or external damage were discarded. They were then identified according to morphological features (Hartwich 1975, Petter & Maillard 1988, Petter & Cabaret 1995.Prior to cultivation, each larva was placed in an antibiotic-antifungal solution (80 mg gentamicin sulfate, 0.625 mg amphotericin B, 10 000 IU sodium penicillin G, 10 mg streptomycin sulfate and 4.5 ml Hanks' solution, for a final solution volume of 10 ml) and axenized as described elsewhere (Iglesias et al. 1997, Iglesias et al. 2001. Worms were cultured in sterile polystyrene 30 ml flasks. The culture medium (10 ml) was placed into each flask with 8 to 10 parasites. The culture flasks were then placed in an incubator at 13°C and 5% CO 2 in humid air, and the culture medium was renewed once a week. The worms were observed daily for motility, moulting and survival. The culture medium (GLI...

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Larval anisakids parasitizing the blue whiting, Micromesistius poutassou, from Motril Bay in the Mediterranean region of southern Spain

Cited by 50 publications

References 16 publications

Hysterothylacium aduncum, the only anisakid parasite of sardines (Sardina pilchardus) from the southern and eastern coasts of Spain

Hysterothylacium aduncum, the only anisakid parasite of sardines (Sardina pilchardus) from the southern and eastern coasts of Spain

Anisakis spp. in European hake, Merluccius merluccius (L.) from the Atlantic off north-west Africa and the Mediterranean off southern Spain

In vitro development of the fish parasite Hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg

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