LARVA TO PHARATE ADULT TRANSFORMATION IN THE MEDFLY <i>CERATITIS CAPITATA</i> (WIEDEMANN) (DIPTERA: TEPHRITIDAE)

Rabossi, Alejandro; Wappner, P.; Allué, Luis Alberto Quesada

doi:10.4039/ent1241139-6

Cited by 28 publications

(33 citation statements)

References 12 publications

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“…Taking advantage of our previous studies on the timing of different events during Medfly metamorphosis (Rabossi et al, 1991(Rabossi et al, , 1992(Rabossi et al, , 2000, in particular during the larva to pupa transition, we have focused our interest in the disintegration and histolysis of the fat body. Although many features of larval fat body disintegration were observed in other dipterans, here we have been able to correlate morphological and biochemical events using the same material.…”

Section: Discussionmentioning

confidence: 99%

“…Former studies on the metamorphosis of the Mediterranean fruit fly, Ceratitis capitata, allowed us to define with high precision the onset, duration and end of pre-pupal, pupal, and pharate adult stages (Rabossi et al, 1992), as well as of several developmental programs like pupariation and pupation (Rabossi et al, 1991). We also established that a temporal correlation exists between the changes in overall lysosomal proteolytic activity and the above events, demonstrating that proteolysis can be taken as an indicator of histolysis progression (Rabossi et al, 2000).…”

Section: Introductionmentioning

confidence: 96%

“…Larval and adult flies were maintained at 23°C and 50-70% relative humidity, with a 16L:8D photoperiod. All the results below refer to time-dependent events occurring during a standard life cycle under these culture conditions (Rabossi et al, 1992). The age of larva III is expressed in hours before puparium formation (BPF).…”

Section: Insectsmentioning

confidence: 99%

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Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis

Rabossi

Stoka

Puizdar

et al. 2004

Arch Insect Biochem Physiol

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During larva to adult transition, the larval fat body of the Medfly (Ceratitis capitata) progressively disintegrates to be replaced by the adult one, after imago ecdysis. Here we show that a temporal correlation exists among the microscopy images of fat body progressive disintegration, the activation of fat body lysosomes (as judged by acid phosphatase activity), and the activity of a novel fat body aspartyl proteinase. The enzyme was purified and partially characterized. This proteinase exhibited a wide range of acid isoforms with isoelectric points from 5.6 to 7.3, an optimum pH of 3.0 for hemoglobin digestion, and was completely inhibited by pepstatin A. The apparent molecular weight was estimated (42 +/- 1 kDa) and the protein was characterized as N-glycosylated, judging from affinity to Concanavalin A. From the biochemical characteristics, the enzyme that we called "Early Metamorphosis Aspartyl Proteinase" (EMAP) appears to be similar to mammalian Cathepsin D. However, the N-terminal sequence of EMAP showed no similarity with any known animal Cathepsins and exhibited an important instability to neutral and alkaline pH. This feature seems to be a peculiar characteristic of insect aspartyl proteinases. The temporal activity profile of EMAP during metamorphosis correlated well with the microscopy images of fat body cell autolytic death. Our data support the notion that EMAP is a metamorphosis-specific lysosomal proteinase, mostly expressed during larval fat body histolysis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis

Rabossi

Stoka

Puizdar

et al. 2004

Arch Insect Biochem Physiol

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“…Some authors accepted that this is the end of the pupation process (Zdarek, 1985), but for C. capitata, we proposed that pupation ends at 72 h APF, when both the definitive shape of the head and final body proportions are attained. At this moment, full deposition of cuticle proteins is also complete (Rabossi et al, 1991(Rabossi et al, , 1992.…”

Section: Archives Of Insect Biochemistry and Physiology May 2008mentioning

confidence: 99%

Purification and characterization of two cysteine peptidases of the Mediterranean fruit fly Ceratitis capitata during metamorphosis

Rabossi

Stoka

Puizdar

et al. 2007

Arch Insect Biochem Physiol

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In holometabolous insects, there is a complete body remodeling from larva to adult. We determined in Ceratitis capitata that the transition from pre-pupa to pupa, 40 to 48 h after puparium formation (h APF), is a key moment of metamorphosis; when salivary glands, intestine, fat body, and muscles are in different stages of cell death. At 44-46 h APF, muscles from segments 1-3 (thoracic region) appeared fully disintegrated, whereas posterior muscles just started death processes. To understand some of the biochemical events eventually involved in histolytic processes during early metamorphosis, two cysteine peptidases coined "Metamorphosis Associated Cysteine Peptidase" (MACP-I and MACP-II) were purified to homogeneity from 40-46-h APF insects. Both enzymes were inhibited by Ep-475, a specific inhibitor of papain-like cysteine-peptidases. MACP-I is a single chain protein with an apparent molecular mass of 80 kDa and includes several isoforms with pI values of pH 6.25-6.35, 6.7, and 7.2. The enzyme has an optimum pH of 5.0 and its pH stability ranges from pH 4.0 to 6.0. The molecular weight and N-terminal sequence suggest that MACP-I might be a novel enzyme. MACP-II is an acidic single chain protein with a pI of pH 5.85 and an apparent molecular mass of 30 kDa. The enzyme is labile with a maximum stability in the pH range of 4.0 to 6.0 and an optimum pH among 5.0 to 6.0. MAPCP-II characteristics suggest it is a cathepsin B-like enzyme.

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“…Reference works for the laboratory rearing techniques of C. capitata are Refs. [2][3][4] and for A. fraterculus is Ref. [5].…”

Section: A Laboratory Rearing Technique Adjusted For Genetic Studiesmentioning

confidence: 99%

Reference Karyotypes and Chromosomal Variability: A Journey with Fruit Flies and the Key to Survival

Abraham¹

2017

Chromosomal Abnormalities - A Hallmark Manifestation of Genomic Instability

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My goal is to present the analysis of concepts on the "normal" karyotype and chromosomal abnormalities through comprehension of chromosomal variation within fruit flies populations, advantages of these insects as models to study genetic polymorphisms and the methodology from field to strains. Cytological preparations were obtained from cerebral ganglion. Staining methods include routine and fluorescent bandings as well as in situ hybridization using DNA probes. We define a more frequent karyotype of each one species and take them as the reference karyotype. The reference as well as the chromosomal variants studied within each species were isolated in different strains. The techniques applied revealed differences among individuals belonging to different strains, thus documenting the mutations into the DNAr cluster, variation in the patterns of heterochromatin, mosaic specimens carrying nuclei with different chromosomal numbers. Hoecht revealed double-minute chromosomes and CG-rich banding marked somatic crossing over between sister chromatids. The most frequent karyotype is the reference karyotype, namely, the normal karyotype. Chromosomal mutations produce variability. In man, a number of these mutants are considered chromosomal abnormalities. We learnt that variation is the key to survival and that many individuals could be in the right place in the wrong moment.

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LARVA TO PHARATE ADULT TRANSFORMATION IN THE MEDFLY CERATITIS CAPITATA (WIEDEMANN) (DIPTERA: TEPHRITIDAE)

Cited by 28 publications

References 12 publications

Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis

Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis

Purification and characterization of two cysteine peptidases of the Mediterranean fruit fly Ceratitis capitata during metamorphosis

Reference Karyotypes and Chromosomal Variability: A Journey with Fruit Flies and the Key to Survival

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