Large Sequence Polymorphisms Unveil the Phylogenetic Relationship of Environmental and Pathogenic Mycobacteria Related to
            <i>Mycobacterium ulcerans</i>

Käser, Michael; Hauser, Julia; Small, P. L. C.; Pluschke, Gerd

doi:10.1128/aem.00446-09

Cited by 30 publications

(24 citation statements)

References 43 publications

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“…Within the M. tuberculosis complex, and even within M. ulcerans-related mycolactone-producing mycobacteria, analysis of LSPs represents a valuable approach for genetic fingerprinting (5,6,12,18). However, with the increasing availability of multiple whole-genome sequences, SNP identification adds considerably to phylogeographic analyses (11,13,14).…”

Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms on the Road to Strain Differentiation in Mycobacterium ulcerans

2009

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The genomic fine-typing of strains of Mycobacterium ulcerans, the causative agent of the emerging human disease Buruli ulcer, is difficult due to the clonal population structure of geographical lineages. Although large sequence polymorphisms (LSPs) resulted in the clustering of patient isolates originating from across the globe, differentiation of strains within continents using conventional typing methods is very limited. In this study, we analyzed M. ulcerans LSP haplotype-specific insertion sequence elements among 83 M. ulcerans strains and identified single nucleotide polymorphisms (SNPs) that differentiate between regional strains. This is the first genetic discrimination based on SNPs of M. ulcerans strains from African countries where Buruli ulcer is endemic, resulting in the highest geographic resolution of genotyping so far. The findings support the concept of genome-wide SNP analyses as tools to study the epidemiology and evolution of M. ulcerans at a local level.

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Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms on the Road to Strain Differentiation in Mycobacterium ulcerans

2009

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“…Primer selection of present study was done based on local characteristics of strains and their importance in the diagnosis of the disease (tabulated in Table 4). The nucleotide sequences of primers used for the detection of Mycobacterium in this study were described and validated as diagnostic markers in the past [17,18,29,[37][38][39][40][41][42][43][44][45][46]. This antigen would provide a target, which is universally present.…”

Section: Resultsmentioning

confidence: 99%

“…All samples collected were examined by H & E staining [30,31,32], Z-N staining for AFB [32,33,34], cultured on L-J egg media [34,35,36] and MTB specific multi-gene PCR method [17,18,29,[37][38][39][40][41][42][43][44][45][46] by which FGTB was confirmed and correlated with laparoscopic findings. The diagnosis was done based on morphological [19] and molecular investigations.…”

Section: Case Groupmentioning

confidence: 99%

Detection of Mycobacterium Tuberculosis among Infertile Patients Suspected with Female Genital Tuberculosis

Bhanothu¹,

Theophilus²,

Rozati³

2014

AJIDM

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Female genital tuberculosis (FGTB) is a symptomless disease that evidences itself only when it is investigated for infertility. Demonstration of the etiologic agent by H & E staining or Z-N staining for acid fast bacilli, smear microscopy, culture of menstrual blood, urine and sputum were often unsuccessful. We therefore, proposed to use the endo-ovarian tissue biopsies and pelvic aspirated fluids for the detection of FGTB among infertile women by conventional versus genotypic methods. A prospective case-control study was undertaken. A total of 302 specimens were collected from 202 infertile women highly suspected of having FGTB on laparoscopic examination and from 100 control women of reproductive age. Out of these 302 specimens, 150 (49.67%) were premenstrual endometrial tissue biopsies (ETBs), 95 (31.46%) were ovarian tissue biopsies (OTBs) and 57 (18.87%) were pelvic aspirated fluids (PAFs). All specimens tested by conventional/ phenotypic methods were later compared with multi-gene/ multi-primer PCR (multi-gene PCR) method using four sets of primers for the detection of Mycobacterium tuberculosis (MTB) DNA in a single tube-single step reaction and correlated with laparoscopic findings. The presence of MTB DNA was observed in 49.5% of ETBs, 33.17% of OTBs and 5.44% of PAF specimens collected from highly suspected FGTB patients. All control women were confirmed as negative for tuberculosis. The conventional methods showed 99% to 100% specificity with a low sensitivity, ranging from 21.78% to 42.08% while H & E staining showed a sensitivity of 51.48%. Multi-gene PCR method was found to have a much higher sensitivity of 70.29% with MTB64 gene, 86.63% with 19kDa antigen gene at species and TRC4 element at regional MTB complex level and 88.12% with 32kDa protein gene at genus level (Pearson χ2 =214.612, 1df, McNemar's test value <0.0001). The specificity of multi-gene PCR was 100%. We suggest site specific sampling, irrespective of sample type and amplification of the 19kDa antigen gene in combination with TRC4 element as a successful multi-gene PCR method for the diagnosis of FGTB and differentiation of mycobacterial infection among endo-ovarian tissue biopsies and PAFs taken from infertile women.

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“…Phylogenetic analyses indicate that M. ulcerans is a close relative of the common marine bacterium Mycobacterium marinum (21)(22)(23). The genome of M. ulcerans is unique in that it includes a high-copy-number insertion sequence (IS) known as IS2404.…”

mentioning

confidence: 99%

“…Three different pMUM plasmids have been identified, and each produces a slightly different form of mycolactone. The taxonomy of mycolactone-producing mycobacteria (MPM) has been extensively evaluated (21,23,(27)(28)(29), most recently by whole-genome sequence comparisons of 30 M. ulcerans and 5 M. marinum isolates (30). The results show that all MPM strains belong to a single species, M. ulcerans.…”

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confidence: 99%

Environmental Distribution and Seasonal Prevalence of Mycobacterium ulcerans in Southern Louisiana

Hennigan

Myers

Ferris

2013

Appl Environ Microbiol

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dMycobacterium ulcerans is an emerging environmental pathogen that causes debilitating, ulcerative disease in humans and other vertebrates. The majority of human cases occur in tropical and temperate regions of Africa and Australia, and outbreaks of piscine mycobacteriosis caused by M. ulcerans have been reported in disparate geographic locations spanning the globe. While exposure to a natural body of water is the most common risk factor for human infection, the environmental distribution of M. ulcerans in aquatic habitats has not been extensively studied. Although no human cases have been reported in the United States, a strain of M. ulcerans has been identified as the cause of a piscine mycobacteriosis in Striped bass (Morone saxatilis) within the Chesapeake Bay. Infected fish exhibit bright red ventral and lateral dermal lesions. We observed a possible outbreak causing similar lesions on red drum (Sciaenops ocellatus) in wetlands of southern Louisiana and detected M. ulcerans-specific genetic markers in lesion samples from these fish. Based on these findings, we studied the geographic and seasonal prevalence of these markers across southern Louisiana. M. ulcerans was detected in each of the nine areas sampled across the state. M. ulcerans prevalence was significantly lower in the fall samples, and the low prevalence coincided with decreased nutrient levels and an increase in water temperature. To our knowledge, this is the first study of M. ulcerans biomarkers in the southern United States. Mycobacterium ulcerans is a pathogenic, toxin-producing bacterium that is the causative agent of Buruli ulcer (BU), a necrotizing skin infection in humans. Although the causative agent was discovered in the mid-1900s, BU remained a largely neglected tropical disease until the World Health Organization (WHO) established the Global Buruli Ulcer Initiative (GBUI) in 1998. M. ulcerans is currently recognized as a rapidly emerging pathogen. BU is endemic in 33 countries, with the highest number of cases occurring in tropical and subtropical regions of west and sub-Saharan Africa and Australia (1). In addition to human cases, M. ulcerans is responsible for disease in a wide range of other host species, including domestic and wild mammals (2-6), reptiles (7), amphibians (8), and fish (9, 10). Strains of M. ulcerans have increasingly been identified as the cause of piscine mycobacteriosis occurring in locations across the globe (9), including Israel (11), Europe (10,12), and the northeastern United States (13,14).Fundamental aspects of the ecology and epidemiology of M. ulcerans, including its environmental distribution, niche, host range, and mode of transmission and infection, are incompletely understood. The most definitive risk factor for BU is frequent contact with natural aquatic systems (15). This risk has been substantiated by studies that have shown that M. ulcerans is detected by PCR in samples collected from slow-flowing or stagnant bodies of water in areas with an incidence of BU (1). Within these aquatic environments,...

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Large Sequence Polymorphisms Unveil the Phylogenetic Relationship of Environmental and Pathogenic Mycobacteria Related to Mycobacterium ulcerans

Cited by 30 publications

References 43 publications

Single Nucleotide Polymorphisms on the Road to Strain Differentiation in Mycobacterium ulcerans

Single Nucleotide Polymorphisms on the Road to Strain Differentiation in Mycobacterium ulcerans

Detection of Mycobacterium Tuberculosis among Infertile Patients Suspected with Female Genital Tuberculosis

Environmental Distribution and Seasonal Prevalence of Mycobacterium ulcerans in Southern Louisiana

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