Design and safety assessment of advanced ductile cast iron (DCI) components like windturbines or transport and storage casks for radioactive materials require appropriate material data interms of strength and fracture toughness. Therefore, it is of vital importance to characterize andunderstand the deformation, damage and fracture behaviour of DCI which may substantially changefrom ductile to brittle by increasing loading rate, decreasing temperature and/or increasing stresstriaxiality. This paper reports on recent BAM investigations on different qualities of the widely usedDCI grade EN-GJS-400 with varying pearlite shares (none and 18 % respectively). The focus wason the influences of microstructure, temperature (ambient and -40 °C) and loading rate (quasi-staticto crash) on strength (YS, UTS, flow curve) and fracture mechanical properties (R-curve, crackinitiation toughness, fracture toughness). Systematic metallographical and fractographical analyseswere performed accompanying the whole test program and a systematics of specific damagebehaviour and fracture mechanisms was derived from the results.