Large-scale separation of clavine alkaloids from Ipomoea muricata by pH-zone-refining centrifugal partition chromatography

Maurya, Anupam; Srivastava, Santosh K.

doi:10.1016/j.jchromb.2009.04.036

Cited by 28 publications

(26 citation statements)

References 21 publications

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“…The isolated alkaloid was analyzed by RP HPLC (Agilent 1120 Compact LC), with Agilent TC-C18 [2] column (4.6× 250 μm) and a particle size of 5 μm by validating the method as devised by Maurya and Santosh 2009 with a slight modification [4]. The mobile phase consisted of acetonitrile and 0.01 M sodium dihydrogen phosphate buffer (15:85, v/v), pH adjusted to 4.0 with HPLC phosphoric acid, flow rate of 1 ml/min and injection volume of 20 μL.…”

Section: Hplc Analysis Of Lysergolmentioning

confidence: 99%

“…Lysergol being a heat and light sensitive drug, when extracted with the help of conventional methods, is obtained in low yields due to its long extraction times and high temperature and sometimes purity remains one of the concerns [3,4]. Therefore, it was desirable to opt for a new extraction process to overcome the limitations of conventional extraction methods.…”

Section: Introductionmentioning

confidence: 99%

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Application of Response Surface Methodology to Microwave-Assisted Extraction of Lysergol from Ipomoea Genus and Its Characterization by RP HPLC-UV

2011

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Objective Ipomoea genus is an excellent source of lysergol which is used as a hypotensive and psychrotopic analgesic. Microwave-assisted extraction (MAE) was employed to extract lysergol from Ipomoea seeds, and response surface methodology (RSM) was applied to predict optimum conditions for extraction. Methodology Box-Behnken statistical design was used to monitor the effect of independent variables like temperature (A-50°C, 60°C, and 70°C), power (B-240, 350, and 700 W), and time (C-10, 20, and 30 min) on a dependent variable/percent yield of lysergol when microwaves were applied. Correlation analysis of the mathematical regression model indicated that a quadratic polynomial model could be employed to optimize the process. Results The optimum extraction conditions in order to obtain the highest lysergol yield were temperature (60°C), power (350 W), and time (20 min). The average actual yield of lysergol under the optimum conditions was found to be 78.0%, whereas the predicted yield under similar conditions was 85.374%. Conclusions The results verified our experiments and successful application of RSM to MAE of lysergol from Ipomoea seeds. The percentage purity of the extracted lysergol was found to be 90% with reference to standard lysergol (97%) with the help of RP HPLC-UV.

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Section: Hplc Analysis Of Lysergolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of Response Surface Methodology to Microwave-Assisted Extraction of Lysergol from Ipomoea Genus and Its Characterization by RP HPLC-UV

2011

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“…Jacquin of the family Convolvulaceae, locally known as "tonkin/tunkin" 16 , has various therapeutic applications such as being used as a purgative, febrifuge, spasmolytic, hypotensive, antibacterial and antifungal agent 17,18 . In addition, the Dominican parochial community has been known to exploit its seeds, stems, and leaves for the treatment of skin ailments for instance chronic and gangrenous wounds, cuts, and blisters due to burns 19 .…”

Section: Introductionmentioning

confidence: 99%

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2016

IJPSR

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Acetylcholinesterase (AChE), a member of α/ß hydrolase protein super family, is the key enzyme in the breakdown of acetylcholine (ACh) -an important neurotransmitter. Inhibition of this enzyme has been considered an auspicious strategy for the treatment of various neurological disorders such as Alzheimer's disease (AD). In this study, crude methanolic Ipomoea muricata seed extract was subjected for analysis of its potential AChE inhibitory activity using a microplate assay based on Ellman's method. I. muricata's inhibitory activity was compared using Donepezil, a prescription drug for AD treatment. Two time intervals were used: 40 minutes and 24 hours. Based on the findings, Donepezil exhibited greater potency (IC50 > 0.005 μg/mL) at both time intervals in contrast to I. muricata (IC50 = 39.67 μg/mL) at 40 minutes and (IC50 > 57.44 μg/mL) at 24 hours. It is important to note that the crude methanolic seed extract of I. muricata has been shown to cause moderate but persistent inhibition of AChE in comparison to Donepezil which has shown a strong but short-lived action. With this concludes that in the near future followed by further studies, I. muricata can be a potential herbal alternative in the symptomatic treatment of AD.

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“…Furthermore, pH-zonerefining technique [12] has been successfully applied to purify many kinds of alkaloids such as lappaconitine [13], vindoline [14], clavine [15], protopine [16],] and so on.…”

Section: Introductionmentioning

confidence: 99%

Semi‐preparative high‐speed counter‐current chromatography separation of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn by pH‐gradient elution

Duanmu

Sun

et al. 2010

J of Separation Science

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A new high-speed counter-current chromatography method for semi-preparative separation and purification of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn was developed by using pH-gradient elution mode. Diethyl ether was used as the stationary phase of the two-phase solvent system and Na(2)HPO(4)/NaH(2)PO(4) buffer solution with pH values of 7.5 and 7.2 in gradient mode as the mobile phase. Consequently, 33 mg of liensinine, 42 mg of isoliensinine, and 67 mg of neferine were obtained from 200 mg of crude extracts. The purities of them were all over 98% as determined by HPLC area normalization method, and the structures were identified by (1)H-NMR and (13)C-NMR.

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Large-scale separation of clavine alkaloids from Ipomoea muricata by pH-zone-refining centrifugal partition chromatography

Cited by 28 publications

References 21 publications

Application of Response Surface Methodology to Microwave-Assisted Extraction of Lysergol from Ipomoea Genus and Its Characterization by RP HPLC-UV

Application of Response Surface Methodology to Microwave-Assisted Extraction of Lysergol from Ipomoea Genus and Its Characterization by RP HPLC-UV

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Semi‐preparative high‐speed counter‐current chromatography separation of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn by pH‐gradient elution

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