Large Scale Screening of Southern African Plant Extracts for the Green Synthesis of Gold Nanoparticles using Microtitre-Plate Method

Elbagory, Abdulrahman M.; Cupido, Christopher N.; Meyer, Mervin; Hussein, Ahmed A.

doi:10.20944/preprints201608.0120.v1

Cited by 12 publications

(9 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Preliminary screening of H. foetidum extract for the synthesis of AuNPs [10], showed promising results, subsequently, it was subjected to purification steps to identify the compounds responsible for the reduction and stabilization of AuNPs. Chromatographic manipulation in parallel with NPs assay formation, two compounds were isolated.…”

Section: Isolation and Identification Of Compoundsmentioning

confidence: 99%

The potential of chalcone-capped gold nanoparticles for the management of diabetes mellitus

Akinfenwa

Pearce

Omoruyi

et al. 2021

Surfaces and Interfaces

Self Cite

View full text Add to dashboard Cite

Searching for new natural bioactive capping agents represent an urgent priority in the green synthesis of metal nanoparticles. Additionaly, the biosaftey of metal nanparticles is a major concern especially in medical applications. Recently, the use of pharmacollogicaly active natural products as capping agents has been deployed to avoid toxic effects during the nanoparticles preparation and to enhance their drugability compared with convential drugs. Helichrysum foetidum is a South African medicinal plant used in folk medicine for the treatment of different human pathologies, and it is known to contain a variety of bioactive compounds. Herein, the total extract and two pure chalcones, helichrysetin and helichrysin, isolated from the same plant were successfully used to synthesize quasi-monodispersed gold nanoparticles in the size range of 2-12 nm. The bio-evaluation of samples indicated that the AuNP/capping agent conjugates are biostable, and have different biological profiles from the total extract/pure compounds. The enzymatic inhibition assays showed significant inhibition by the total extract, helichrysetin and their gold nanoparticles. Interestingly, a similar activity was observed for glucose uptake in HEK293 treated cells. On the other hand, all the tested samples relatively demonstrated no cytotoxicity when tested against the HaCaT keratinocytes. In conclusion, the study demonstrated potential enhancement of glucose uptake in mammalian kidney cells, and inhibition of carbohydrate-hydrolysing enzymes by green synthesized gold nanoparticles of H. foetidum. It also provides a therapeutic appraisal of AuNPs/chalcones conjugate towards the development of antidiabetes drugs derived from H. foetidum and its gold nanoparticles.

show abstract

Section: Isolation and Identification Of Compoundsmentioning

confidence: 99%

The potential of chalcone-capped gold nanoparticles for the management of diabetes mellitus

Akinfenwa

Pearce

Omoruyi

et al. 2021

Surfaces and Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additionally, gold nanoparticles were mostly mono-dispersed and possessed spherical morphologies with an average particle size of 45 nm ( Table S15 ). On the other hand, numerous studies experienced different shapes, including triangular, pentagonal and hexagonal structures [ 136 , 137 , 138 ]. Furthermore, the clustering and polydispersity of particles were the main obstacles faced by researchers during the biosynthesis of gold nanocrystals [ 139 , 140 ].…”

Section: Green Synthesis Of Transitional Metals and Their Oxidesmentioning

confidence: 99%

Green Synthesis of Transition-Metal Nanoparticles and Their Oxides: A Review

2021

View full text Add to dashboard Cite

In recent years, many researchers have begun to shift their focus onto the synthesis of nanomaterials as this field possesses immense potential that may provide incredible technological advances in the near future. The downside of conventional synthesis techniques, such as co-precipitation, sol-gel and hydrothermal methods, is that they necessitate the use of toxic chemicals, produce harmful by-products and require a considerable amount of energy; therefore, more sustainable fabrication routes are sought-after. Biological molecules have been previously utilized as precursors for nanoparticle synthesis, thus eliminating the negative factors involved in traditional methods. In addition, transition-metal nanoparticles possess a wide scope of applications due to their multiple oxidation states and large surface areas, thereby allowing for a higher reactivity when compared to their bulk counterpart and rendering them an interesting research topic. However, this field is still relatively unknown and unpredictable as the biosynthesis of these nanostructures from fungi, bacteria and plants yield undesired diameters and morphologies, rendering them as redundant when compared to their chemically synthesized counterparts. Therefore, this review aims to obtain a better understanding on the plant-mediated synthesis process of the major transition-metal and transition-metal oxide nanoparticles, and how process parameters—concentration, temperature, contact time, pH level, and calcination temperature affect their unique properties such as particle size, morphologies, and crystallinity.

show abstract

“…Zeta potential is a vital measurement or parameter used to ascertain long-term stability and analytical assessment of nanoparticles [51]. Zeta potential of the quantum dots was not significantly different from each other and with a mean value of (34.12 ± 0.8), (30.87 ± 1.18) and (33.56 ± 1.3), Particles with zeta potential value of above ±30 are said to be very stable according to the literature [52]. The zeta potential of the QDs was above ±30 with QD-GA12 having the highest zeta value and QD-GA2 having the lowest zeta potential.…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Synthesis and cytotoxic evaluation of gum arabic surface modified cadmium telluride quantum dots

Edwina¹,

Kiplagat²,

Sibuyi³

et al. 2020

mat express

Self Cite

View full text Add to dashboard Cite

Water-soluble cadmium telluride (CdTe) quantum dots (QDs) were capped with gum Arabic (GA) is a non-toxic, water-soluble glycoprotein polymer commonly used in the food and pharmaceutical industries. The GA was used to stabilise cadmium telluride quantum dots (GA-QDs) and provides functional groups for other molecules such as nucleic acids, peptides and antibodies to be attached to the QDs for biological and biomedical appli- cations. In this study, the GA was used to cap and stabilise QDs using two different methods. These QDs were characterised using Ultraviolet-visible (UV-vis) and Photoluminescence (PL) spectroscopy, X-powder ray diffraction (XRD), High-resolution transmission electron microscopy, zeta potential and particle size distribu- tions. Cytotoxicity of these QDs was also investigated using four different human cell lines; HeLa, MCF-7, PC-3 and U87 cancer cells. The QDs-MPA was capped with 3-mercaptopropionic acid, QDs-GA2 was stabilized and capped with GA at 60 °C for two hours, and QDs-GA12 was stabilized and capped with GA for twelve hours at room temperature (25 °C) with continuous stirring; These QDs were found to be highly luminescent with PL values of 675 nm, 678 nm and 677 nm respectively. The average polydispersity index (PDI) were 0.36 ± 0.02, 0.27 ± 0.02, 0.35 ± 0.01 for QDs-MPA, QDs-GA2 and QDs-GA12, respectively. The average particles size from HRTEM, XRD and hydrodynamic size showed that the QDs-GA have bigger particles sizes; (56.12 nm ± 1.14), (68.69 nm ± 2.08) and (77.85 nm ± 1.69) for QDs-MPA, QD-GA2 and QD-GA12 respectively. Cytotoxicity studies of these QDs were carried out using WST-1 cell proliferation assay on four different tumour cell line. The results showed that these cells were over 50 per cent viable and the QDs-GA capped had higher cell percentage viability.

show abstract

Large Scale Screening of Southern African Plant Extracts for the Green Synthesis of Gold Nanoparticles using Microtitre-Plate Method

Cited by 12 publications

References 37 publications

The potential of chalcone-capped gold nanoparticles for the management of diabetes mellitus

The potential of chalcone-capped gold nanoparticles for the management of diabetes mellitus

Green Synthesis of Transition-Metal Nanoparticles and Their Oxides: A Review

Synthesis and cytotoxic evaluation of gum arabic surface modified cadmium telluride quantum dots

Contact Info

Product

Resources

About