Large-scale screening of selectedCandida maltosa,Debaryomyces hanseniiandPichia anomalakiller toxin activity against pathogenic yeasts

Buzzini, Pietro; Martini, Alessandro

doi:10.1080/mmy.39.6.479.482

Cited by 30 publications

(9 citation statements)

References 30 publications

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“…Pichia kluyveri and related species (e.g., Wickerhamomyces anomalus , P. fermentans , etc.) are widely studied with respect to wine fermentation as well as biological control, mostly of fungal postharvest diseases of fruits [30, 96–101]. …”

Section: Discussionmentioning

confidence: 99%

Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi

et al. 2017

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BackgroundWhile recent advances in next generation sequencing technologies have enabled researchers to readily identify countless microbial species in soil, rhizosphere, and phyllosphere microbiomes, the biological functions of the majority of these species are unknown. Functional studies are therefore urgently needed in order to characterize the plethora of microorganisms that are being identified and to point out species that may be used for biotechnology or plant protection. Here, we used a dual culture assay and growth analyses to characterise yeasts (40 different isolates) and their antagonistic effect on 16 filamentous fungi; comprising plant pathogens, antagonists, and saprophytes.ResultsOverall, this competition screen of 640 pairwise combinations revealed a broad range of outcomes, ranging from small stimulatory effects of some yeasts up to a growth inhibition of more than 80% by individual species. On average, yeasts isolated from soil suppressed filamentous fungi more strongly than phyllosphere yeasts and the antagonistic activity was a species-/isolate-specific property and not dependent on the filamentous fungus a yeast was interacting with. The isolates with the strongest antagonistic activity were Metschnikowia pulcherrima, Hanseniaspora sp., Cyberlindnera sargentensis, Aureobasidium pullulans, Candida subhashii, and Pichia kluyveri. Among these, the soil yeasts (C. sargentensis, A. pullulans, C. subhashii) assimilated and/or oxidized more di-, tri- and tetrasaccharides and organic acids than yeasts from the phyllosphere. Only the two yeasts C. subhashii and M. pulcherrima were able to grow with N-acetyl-glucosamine as carbon source.ConclusionsThe competition assays and physiological experiments described here identified known antagonists that have been implicated in the biological control of plant pathogenic fungi in the past, but also little characterised species such as C. subhashii. Overall, soil yeasts were more antagonistic and metabolically versatile than yeasts from the phyllosphere. Noteworthy was the strong antagonistic activity of the soil yeast C. subhashii, which had so far only been described from a clinical sample and not been studied with respect to biocontrol. Based on binary competition assays and growth analyses (e.g., on different carbon sources, growth in root exudates), C. subhashii was identified as a competitive and antagonistic soil yeast with potential as a novel biocontrol agent against plant pathogenic fungi.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0908-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi

et al. 2017

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“…The antimycotic activity spectrum of both PBE and TA was evaluated by using the agar diffusion well bioassay (ADWB) [21,42]. Amphotericin B (AmB) and ketoconazole (Keto) (Calbiochem Inc., USA) were also tested as antimycotic control agents.…”

Section: Determination Of the Antimycotic Activity Spectrummentioning

confidence: 99%

“…strains, belonging to either well-known or emerging pathogenic species [39][40][41][42] were used as target microorganisms. All strains are conserved in the Industrial Yeast Collection DBVPG, University of Perugia, Italy, www.agr.unipg.it/dbvpg.…”

Section: Microorganisms and Mediamentioning

confidence: 99%

Analysis of condensed and hydrolysable tannins from commercial plant extracts

Romani

Ieri

Turchetti

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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“…Moreover, P. anomala enhanced the nutritional quality of the feed by increasing protein content and reducing the concentration of the antinutritional compound phytate [98]. The killer activity of P. anomala is also of interest in biomedical applications due to its activity against potential pathogenic yeast species, which may lead to the development of new antimycotic agents [72,99].…”

Section: Applications Of W Anomalus Beyond Wine Fermentationmentioning

confidence: 99%

Challenges of the Non-Conventional Yeast Wickerhamomyces anomalus in Winemaking

2018

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Abstract:Nowadays it is widely accepted that non-Saccharomyces yeasts, which prevail during the early stages of alcoholic fermentation, contribute significantly to the character and quality of the final wine. Among these yeasts, Wickerhamomyces anomalus (formerly Pichia anomala, Hansenula anomala, Candida pelliculosa) has gained considerable importance for the wine industry since it exhibits interesting and potentially exploitable physiological and metabolic characteristics, although its growth along fermentation can still be seen as an uncontrollable risk. This species is widespread in nature and has been isolated from different environments including grapes and wines. Its use together with Saccharomyces cerevisiae in mixed culture fermentations has been proposed to increase wine particular characteristics. Here, we review the ability of W. anomalus to produce enzymes and metabolites of oenological relevance and we discuss its potential as a biocontrol agent in winemaking. Finally, biotechnological applications of W. anomalus beyond wine fermentation are briefly described.

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Large-scale screening of selectedCandida maltosa,Debaryomyces hanseniiandPichia anomalakiller toxin activity against pathogenic yeasts

Cited by 30 publications

References 30 publications

Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi

Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi

Analysis of condensed and hydrolysable tannins from commercial plant extracts

Challenges of the Non-Conventional Yeast Wickerhamomyces anomalus in Winemaking

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