Large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection

Li, Ze-Yuan; Xia, Jing; Chen, Zheng; Yu, Yang; Li, Quanfeng; Zhang, Yuchan; Zhang, Jinping; Wang, Congying; Zhu, Xiaoyuan; Zhang, Weixiong; Chen, Yue‐Qin

doi:10.1038/srep25493

Cited by 26 publications

(17 citation statements)

References 58 publications

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“…MicroRNAs (miRNAs) are small noncoding RNAs that mediate post‐transcriptional gene silencing through degradation or translational repression of target transcripts (Llave et al , ; Brodersen et al , ). They are essential regulators of gene expression in plant developmental processes and adaptation to biotic and abiotic stress (Navarro et al , ; Chen, ; Li et al , ; ; Shivaprasad et al , ; Campo et al , ; Jeong and Green, ; Baldrich et al , ; Baldrich and San Segundo, ; Song et al , ). Evidence also supports a regulation of nutrient homeostasis by certain miRNAs in plants (Liang et al , ; Paul et al , ; Chien et al , ).…”

Section: Introductionmentioning

confidence: 99%

Phosphate excess increases susceptibility to pathogen infection in rice

Campos-Soriano

Bundó

Bach‐Pages

et al. 2020

Molecular Plant Pathology

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Phosphorus (P) is an essential nutrient for plant growth and productivity. Due to soil fixation, however, phosphorus availability in soil is rarely sufficient to sustain high crop yields. The overuse of fertilizers to circumvent the limited bioavailability of phosphate (Pi) has led to a scenario of excessive soil P in agricultural soils. Whereas adaptive responses to Pi deficiency have been deeply studied, less is known about how plants adapt to Pi excess and how Pi excess might affect disease resistance. We show that high Pi fertilization, and subsequent Pi accumulation, enhances susceptibility to infection by the fungal pathogen Magnaporthe oryzae in rice. This fungus is the causal agent of the blast disease, one of the most damaging diseases of cultivated rice worldwide. Equally, MIR399f overexpression causes an increase in Pi content in rice leaves, which results in enhanced susceptibility to M. oryzae. During pathogen infection, a weaker activation of defence‐related genes occurs in rice plants over‐accumulating Pi in leaves, which is in agreement with the phenotype of blast susceptibility observed in these plants. These data support that Pi, when in excess, compromises defence mechanisms in rice while demonstrating that miR399 functions as a negative regulator of rice immunity. The two signalling pathways, Pi signalling and defence signalling, must operate in a coordinated manner in controlling disease resistance. This information provides a basis to understand the molecular mechanisms involved in immunity in rice plants under high Pi fertilization, an aspect that should be considered in management of the rice blast disease.

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Section: Introductionmentioning

confidence: 99%

Phosphate excess increases susceptibility to pathogen infection in rice

Campos-Soriano

Bundó

Bach‐Pages

et al. 2020

Molecular Plant Pathology

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“…lycopersici , which causes vascular wilt disease in tomato (Ouyang et al, 2014). In rice, many miRNAs are also proposed to be involved in regulation of immunity against Magnaporthe oryzae , although the mechanism is largely unknown (Li et al, 2014, 2016). …”

Section: Introductionmentioning

confidence: 99%

“…In rice, different miR169 isoforms are differentially accumulated in the resistant and susceptible accessions upon M . oryzae infection (Li et al, 2014, 2016), and are differentially responsive to elicitor treatment (Campo et al, 2013; Baldrich et al, 2015), indicating the involvement of miR169/NF-YA modules in rice responses to M . oryzae .…”

Section: Introductionmentioning

confidence: 99%

Osa-miR169 Negatively Regulates Rice Immunity against the Blast Fungus Magnaporthe oryzae

et al. 2017

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miR169 is a conserved microRNA (miRNA) family involved in plant development and stress-induced responses. However, how miR169 functions in rice immunity remains unclear. Here, we show that miR169 acts as a negative regulator in rice immunity against the blast fungus Magnaporthe oryzae by repressing the expression of nuclear factor Y-A (NF-YA) genes. The accumulation of miR169 was significantly increased in a susceptible accession but slightly fluctuated in a resistant accession upon M. oryzae infection. Consistently, the transgenic lines overexpressing miR169a became hyper-susceptible to different M. oryzae strains associated with reduced expression of defense-related genes and lack of hydrogen peroxide accumulation at the infection site. Consequently, the expression of its target genes, the NF-YA family members, was down-regulated by the overexpression of miR169a at either transcriptional or translational level. On the contrary, overexpression of a target mimicry that acts as a sponge to trap miR169a led to enhanced resistance to M. oryzae. In addition, three of miR169’s target genes were also differentially up-regulated in the resistant accession upon M. oryzae infection. Taken together, our data indicate that miR169 negatively regulates rice immunity against M. oryzae by differentially repressing its target genes and provide the potential to engineer rice blast resistance via a miRNA.

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“…Meanwhile, the rice-blast system has been developed as a model to study the mechanism of pathogen-associated molecular pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) in plant-fungus interaction (Andolfo and Ercolano, 2015; Stael et al, 2015). To date, the underlying molecular mechanism of rice resistance to diseases has been illustrated in multiple levels, including transcriptome, proteome, post-transcriptional modification and epigenetic regulation (Miah et al, 2013; Xu et al, 2015; Li et al, 2016; Sharma et al, 2016). Various regulatory factors mediating the blast resistance in rice have been identified by a combination of biochemical, genetic and high-throughput sequencing approaches.…”

Section: Introductionmentioning

confidence: 99%

4-Coumarate-CoA Ligase-Like Gene OsAAE3 Negatively Mediates the Rice Blast Resistance, Floret Development and Lignin Biosynthesis

Líu

Guo

et al. 2017

Front. Plant Sci.

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Although adenosine monophosphate (AMP) binding domain is widely distributed in multiple plant species, detailed molecular functions of AMP binding proteins (AMPBPs) in plant development and plant-pathogen interaction remain unclear. In the present study, we identified an AMPBP OsAAE3 from a previous analysis of early responsive genes in rice during Magnaporthe oryzae infection. OsAAE3 is a homolog of Arabidopsis AAE3 in rice, which encodes a 4-coumarate-Co-A ligase (4CL) like protein. A phylogenetic analysis showed that OsAAE3 was most likely 4CL-like 10 in an independent group. OsAAE3 was localized to cytoplasm, and it could be expressed in various tissues. Histochemical staining of transgenic plants carrying OsAAE3 promoter-driven GUS (β-glucuronidase) reporter gene suggested that OsAAE3 was expressed in all tissues of rice. Furthermore, OsAAE3-OX plants showed increased susceptibility to M. Oryzae, and this finding was attributable to decreased expression of pathogen-related 1a (PR1) and low level of peroxidase (POD) activity. Moreover, OsAAE3 over-expression resulted in increased content of H2O2, leading to programmed cell-death induced by reactive oxygen species (ROS). In addition, OsAAE3 over-expression repressed the floret development, exhibiting dramatically twisted glume and decreased fertility rate of anther. Meanwhile, the expressions of lignin biosynthesis genes were significantly decreased in OsAAE3-OX plants, thereby leading to reduced lignin content. Taken together, OsAAE3 functioned as a negative regulator in rice blast resistance, floret development, and lignin biosynthesis. Our findings further expanded the knowledge in functions of AMBPs in plant floret development and the regulation of rice-fungus interaction.

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Large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection

Cited by 26 publications

References 58 publications

Phosphate excess increases susceptibility to pathogen infection in rice

Phosphate excess increases susceptibility to pathogen infection in rice

Osa-miR169 Negatively Regulates Rice Immunity against the Blast Fungus Magnaporthe oryzae

4-Coumarate-CoA Ligase-Like Gene OsAAE3 Negatively Mediates the Rice Blast Resistance, Floret Development and Lignin Biosynthesis

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