Large-scale production of functional human serum albumin from transgenic rice seeds

Abstract

Human serum albumin (HSA) is widely used in clinical and cell culture applications. Conventional production of HSA from human blood is limited by the availability of blood donation and the high risk of viral transmission from donors. Here, we report the production of Oryza sativa recombinant HSA (OsrHSA) from transgenic rice seeds. The level of OsrHSA reached 10.58% of the total soluble protein of the rice grain. Large-scale production of OsrHSA generated protein with a purity >99% and a productivity rate of 2.75 g/kg brown rice. Physical and biochemical characterization of OsrHSA revealed it to be equivalent to plasma-derived HSA (pHSA). The efficiency of OsrHSA in promoting cell growth and treating liver cirrhosis in rats was similar to that of pHSA. Furthermore, OsrHSA displays similar in vitro and in vivo immunogenicity as pHSA. Our results suggest that a rice seed bioreactor produces cost-effective recombinant HSA that is safe and can help to satisfy an increasing worldwide demand for human serum albumin.

Chemically Modified Human Serum Albumin Potently Blocks Entry of Ebola Pseudoviruses and Viruslike Particles

“…Highly pure (Ͼ99%) recombinant HSA can expediently be obtained from transgenic rice seeds (2.75 g HSA per kg of brown rice) (44), making it possible to produce HP-HSA on a large scale. In contrast, for antibody production in plants, which is the cheapest approach to antibody production, only 500 mg antibody per kg of fresh biomass of Nicotiana benthamiana leaves can be prepared (45).…”

7. Themenbereich grüne Gentechnologie: Pflanzenzüchtung und Agrarwirtschaft

“…Für eine Vielzahl verschiedener Krankheiten wie Gebärmutterhalskrebs, Tuberkulose, Hepatits B, Cholera, HIV sowie Durchfallerkrankungen und viele weitere werden weltweit PMPs entwickelt. 80 Die Gewinnung von humanem Serum Albumin (HSA) aus Reis ist chinesischen Forschern gelungen (He et al, 2011 Clemente/Corigliano, 2012;Waheed et al, 2012;Ahmad et al, 2012;Yusibov et al, 2011. setzt wurde und Wirksamkeit gegenüber dem Malaria-Erreger Plasmodium falciparum zeigte (Jones et al, 2013). Eine umfangreiche Darstellung bisheriger Arbeiten zur Produktion von Vakzinen in transgenen Pflanzen findet sich in Guan et al (2013).…”

Plant Molecular Farming: Much More than Medicines

“…For transgenic plants and transient expression platforms, the environment of the plant can have a substantial impact; for example, differences in temperature of only 1-2 • C can change the yields of a recombinant protein by up to 15% (30). The combination of good construct design, optimal genetic background, and a supportive environment has achieved extraordinary yields of up to 10.6% of the total soluble protein (TSP) for human serum albumin expressed in rice seeds (31), 30% TSP for industrial enzymes expressed in maize seeds (32), 36% TSP for a murine antibody expressed in Arabidopsis (33), and more than 70% TSP for proteins expressed in tobacco chloroplasts (34).…”