Large-scale production in Pichia pastoris of the recombinant vaccine Gavac™ against cattle tick

Canales, Mario; Enríquez, A.; Ramos, Eduardo Antônio Gonçalves; Cabrera, Deborah; Dandie, Hubert; Hernández-Soto, Alejandro; Falcón, Viviana; Rodrı́guez, Manuel; Fuente, José de la

doi:10.1016/s0264-410x(96)00192-2

Cited by 167 publications

(123 citation statements)

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“…All studies had a completely randomized design except one (CANALES et al, 1997), which failed to provide data regarding the experimental design. The number of animals per group, considering each animal to be one replicate in each assay, ranged from 3 to 16, and the vast majority (21/25) used 3 to 6 animals per group.…”

Section: Resultsmentioning

confidence: 99%

“…The number of animals per group, considering each animal to be one replicate in each assay, ranged from 3 to 16, and the vast majority (21/25) used 3 to 6 animals per group. Two authors failed to report the number of animals per group (CANALES et al, 1997;FRAGOSO et al, 1998), and three articles used groups with different numbers of animals (ANDREOTTI, 2006;AZHAHIANAMBI et al, 2009a;KUMAR et al, 2012a). Three studies included an unvaccinated negative control group, 21 studies vaccinated with an equal volume of saline and/or adjuvant, and one study failed to report the negative control treatment (CANALES et al, 1997).…”

Section: Resultsmentioning

confidence: 99%

“…The latter variable was calculated based on the weight of the eggs from the vaccinated group compared to the weight of the eggs from the control group. Eight years later, Canales et al (1997) calculated for the first time the proper efficacy by considering the following three variables for its calculation: total tick production, capacity of oviposition and fertility. Subsequently, Fragoso et al (1998) used these measures when testing the commercial vaccine Gavac TM (Heber Biotec S.A., P.O.…”

Section: Introductionmentioning

confidence: 99%

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Calculation of the efficacy of vaccines against tick infestations on cattle

Cunha

Andreotti

Garcia

et al. 2013

Rev. Bras. Parasitol. Vet.

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Cattle ticks are responsible for great economic losses in cattle farming worldwide, and their main control method, chemicals, has been showing problems, whether resulting from the development of resistant strains of ticks or environmental contamination. Research studies directed toward developing vaccines against ticks are emerging. One way to evaluate those vaccines is to calculate the percentage of efficacy. The aim of this study was to analyze scientific publications archived in PubMed that used this method of assessment and discuss the main factors that may affect its calculation. Thus, 25 articles addressing this subject were selected. The percentage of efficacy was usually calculated in one of two ways, with one considering the reduced fertility of eggs and the other not. The latter method may underestimate the vaccine efficacy, and the most complete formula for calculating the efficacy reflects how much the vaccine actually affects the infestation. In our view, the use of the complete formula for calculating the percentage of efficacy is broader and more representative of the vaccine effect on the tick population.Keywords: Efficiency, ticks, vaccine, bovine. ResumoCarrapatos de bovinos são responsáveis por grandes perdas econômicas para a pecuária bovina mundial e seu principal método de controle, o químico, vem apresentando problemas, seja pelo desenvolvimento de amostras de carrapatos resistentes ou pela contaminação ambiental. Na tentativa de diminuir a utilização dos acaricidas, surgem pesquisas direcionadas ao desenvolvimento de vacinas contra carrapatos. Uma maneira de avaliar essas vacinas é pelo cálculo de percentagem de eficácia. O objetivo deste trabalho foi analisar as publicações científicas indexadas no PubMed que utilizaram este método de avaliação e discutir os principais fatores que podem interferir no seu cálculo. Dessa maneira, selecionaram-se 25 artigos que tratavam desse assunto. A percentagem de eficácia apareceu sendo calculada de duas formas, uma considerando a redução da fertilidade dos ovos e a outra não. Essa última pode subestimar a eficiência da vacina, e a fórmula de cálculo da eficácia mais completa representa o quanto da infestação a vacina realmente reduziu. Em nosso entendimento, a utilização da fórmula completa para o cálculo da percentagem de eficácia é mais abrangente e representativa do efeito da vacina na população de carrapatos.Palavras-chave: Eficiência, carrapato, vacina, bovino.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Calculation of the efficacy of vaccines against tick infestations on cattle

Cunha

Andreotti

Garcia

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…Consequently, two Bm86-based vaccines, GAVAC TM and TickGARD TM , were commercialized and used for cattle vaccination (Willadsen et al, 1995;Canales et al, 1997;de la Fuente et al, 1998). Initial reports on the efficacy of Bm86 vaccines were highly promising,…”

Section: Introductionmentioning

confidence: 99%

Comparative microarray analyses of adult female midgut tissues from feeding Rhipicephalus species

Zyl

Stutzer

Olivier

et al. 2015

Ticks and Tick-borne Diseases

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The cattle tick, Rhipicephalus microplus, has a debilitating effect on the livestock industry worldwide, owing to its being a vector of the causative agents of bovine babesiosis and anaplasmosis. In South Africa, co-infestation with R. microplus and R. decoloratus, a common vector species on local livestock, occurs widely in the northern and eastern parts of the country. An alternative to chemical control methods is sought in the form of a tick vaccine to control these tick species. However, sequence information and transcriptional data for R. decoloratus is currently lacking.Therefore, this study aimed at identifying genes that are shared between midgut tissues of feeding adult female R. microplus and R. decoloratus ticks. In this regard, a custom oligonucleotide microarray comprising of 13,477 R. microplus sequences was used for transcriptional profiling and 2,476 genes were found to be shared between these Rhipicephalus species. In addition, 136 transcripts were found to be more abundantly expressed in R. decoloratus and 1,084 in R. microplus. Chi-square analysis revealed that genes involved in lipid transport and metabolism are significantly overrepresented in R. microplus and R. decoloratus. This study is the first transcriptional profiling of R. decoloratus and is an additional resource that can be evaluated further in future studies for possible tick control.2

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“…[6][7][8] The Bm86 antigen is expressed in the methylotrophic yeast Pichia pastoris, and the recombinant protein is obtained as a highly glycosylated and particulate antigen. 3,9 These characteristics of submit your manuscript | www.dovepress.com…”

Section: Introductionmentioning

confidence: 99%