Large-scale mutagenesis in the zebrafish: in search of genes controlling development in a vertebrate

Mullins, Mary C.; Hammerschmidt, Matthias; Haffter, P.; Nüsslein‐Volhard, Christiane

doi:10.1016/s0960-9822(00)00048-8

Cited by 702 publications

(478 citation statements)

References 25 publications

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“…Fish were fed twice daily and housed as described elsewhere (Mullins et al, 1994) using protocols approved by the McGill University Animal Care Committee. Embryos to be used for developmental studies were collected and staged according to conventional criteria (Kimmel et al, 1995) and by hours post fertilization (hpf).…”

Section: Experimental Procedures Fish Husbandrymentioning

confidence: 99%

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Chitramuthu

Baranowski

Cadieux

et al. 2010

Developmental Dynamics

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Pro-protein convertase subtilisin/kexin 5 (PC5, also known as PC6) is a member of the subtilisin-like superfamily of serine proteases implicated in the maturation of latent precursor proteins into their functionally active derivatives. To investigate the functional roles, we have cloned the cDNA sequences encoding two candidate zebrafish PC5 convertases (designated as PCSK5.1 and PCSK5.2) co-orthologous to the single PC5 encoding gene (PCSK5) found in mammals. Both display syntenic correspondence to the human PCSK5 gene. Overall gene architecture has been conserved across species. While PC5.1 mRNA expression is very discrete within the otic vesicle and lateral line neuromasts, PC5.2 transcripts are more ubiquitously expressed within the central nervous system together with specific localization in various organs including liver, intestine, and otic vesicle. Zebrafish PC5.1-deficient embryos display abnormal neuromast deposition within the lateral line system and lack a normal touch response, consistent with the known sensory role that the lateral line plays in spatial awareness and sensing the environment.

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Section: Experimental Procedures Fish Husbandrymentioning

confidence: 99%

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Chitramuthu

Baranowski

Cadieux

et al. 2010

Developmental Dynamics

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“…The mutant strains presently are preserved as cryopreserved sperm. It takes the period of one generation to recover the mutant individuals with the desired phenotype from the cryopreserved sperm, while two cycles of generation are necessary in the maternal-effect mutant (Mullins et al, 1994: Dosch et al, 2004. In strains with recessive mutation, just one-forth individuals with the mutant phenotype are obtained from the crossing of the parent fish with the heterozygous genotype.…”

Section: Preservation Of the Mutant Strainmentioning

confidence: 99%

Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes

Yamaha

Saito

Goto-Kazeto

et al. 2007

Journal of Sea Research

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The purpose of this review is to introduce surrogate production as a new technique for fish-seed production in aquaculture. Surrogate production in fish is a technique used to obtain the gametes of a certain genotype through the gonad of another genotype. It is achieved by inducing germ-line chimerism between different species during early development. Primordial germ cells (PGCs) are a key material of this technique to induce germ-line chimera. In several species, it has been reported that PGCs differentiated from the blastomeres inherited some maternally supplied mRNA located at the terminal regions of the early cleavage furrows. PGCs from donor species (or strains) are isolated and transplanted into host species to induce the germ-line chimera.Four methods for inducing germ-line chimera, namely, blastomere transplantation, blastoderm-graft transplantation, transplantation of PGC from the genital ridge and transplantation visualized PGC with GFP fluorescence, are described. Several problems preventing the successful induction of germ-line chimera in various fish species are mentioned. Surrogate production, however, has the possibility to allow efficient fish-seed production and effective breeding and transfer of biodiversity to an aquaculture strain.Conservation and efficient utilization of genetic resources will be achieved through surrogate production combined with the cryopreservation of PGCs.

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“…Adult male zebrafish from an outbred wild-type colony (Mi fish line) were mutagenized with N-ethyl-N-nitrosurea (ENU, Sigma) according to established procedures (Mullins et al, 1994) followed by a three-generation breeding scheme to identify zygotic recessive mutations. The mutagenesis and screening was performed at the University of Michigan.…”

Section: Mutagenesis Screen and Fish Carementioning

confidence: 99%

Zebrafish dou yan mutation causes patterning defects and extensive cell death in the retina

Catalano

Raymond

Goldman

et al. 2007

Developmental Dynamics

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The size of an organ is largely determined by the number of cells it contains, which in turn is regulated by two opposing processes, cell proliferation and cell death, however, it is generally not clear how cell proliferation and cell death are coordinated during development. Here, we characterize the zebrafish dou yan mi234 mutation that results in a dramatic reduction of retinal size and a disruption of retinal differentiation and lamination. The retinal size reduction is caused by increased retinal cell death in a non-cell-autonomous manner during early development. The phenotypic defect in dou yan mi234 arises coincident with the onset of retinal neurogenesis and differentiation. Interestingly, unlike many other small eye mutations, the mutation does not increase the level of cell death in the brain, suggesting that the brain and retina use different mechanisms to maintain cell survival. Identification and further study of the dou yan gene will enhance our understanding of the molecular mechanisms regulating retinal cellular homeostasis, i.e., the balance between cell proliferation and cell death. Developmental Dynamics 236: 1295-1306, 2007.

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Large-scale mutagenesis in the zebrafish: in search of genes controlling development in a vertebrate

Cited by 702 publications

References 25 publications

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes

Zebrafish dou yan mutation causes patterning defects and extensive cell death in the retina

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