Large-Scale Isolation of Sinusoidal Endothelial Cells from Pig and Human Liver

Gerlach, Jörg C.; Zeilinger, Katrin; Spatkowski, Gabriele; Hentschel, Florian; Schnoy, N.; Kolbeck, Stefan; Schindler, R; Neuhaus, P.

doi:10.1006/jsre.2001.6224

Cited by 25 publications

(17 citation statements)

References 31 publications

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“…This procedure routinely yields between 500 and 1,500 viable cells per preparation, consistent with published studies using human livers not needed for transplantation requirements. 14 The sinusoidal phenotype of our cells has been confirmed by electron microscopy to demonstrate presence of fenestrations 15 and by staining to show cytoplasmic uptake of acetylated low-density lipoprotein (acLDL) 16 and expression of lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1) 17,18 and liver-specific ICAM-3 grabbing nonintegrin (L-SIGN). 19 Culture of Human Hepatocyte Cell Line.…”

Section: Methodsmentioning

confidence: 74%

Lymphocyte traffic through sinusoidal endothelial cells is regulated by hepatocytes

et al. 2005

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Crosstalk between hepatic sinusoidal ECs and closely juxtaposed hepatocytes via vascular endothelial growth factor is essential for the maintenance of sinusoidal endothelial growth and differentiation. We propose that paracrine interactions between endothelial cells and hepatocytes also may be responsible for the unique complement of adhesion receptors expressed on sinusoidal endothelium that regulate the recruitment of lymphocytes into the liver. To address this hypothesis, we developed an in vitro model of the hepatic sinusoid in which flowing lymphocytes could interact with hepatic endothelium conditioned by the presence of hepatocytes. Human hepatic sinusoidal endothelial cells cocultured with hepatocytes were activated so that they supported the adhesion of lymphocytes at levels equivalent to those seen on endothelium stimulated with the inflammatory cytokine tumour necrosis factor-␤.

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Section: Methodsmentioning

confidence: 74%

Lymphocyte traffic through sinusoidal endothelial cells is regulated by hepatocytes

et al. 2005

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“…Sinusoidal endothelial cells have a unique phenotype, characterized by a lack of Wiebel Palade bodies and consequent failure to express P-selectin and low levels of vWF even in response to inflammation (1,20,35,68,70) in their mature differentiated state. This combined with the relatively modest shear rate within the sinusoids provides a unique environment for platelet and leukocyte adhesion.…”

Section: Discussionmentioning

confidence: 99%

Hepatic sinusoidal endothelium avidly binds platelets in an integrin-dependent manner, leading to platelet and endothelial activation and leukocyte recruitment

Lalor

Herbert

Bicknell

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Uptake of microbeads has been used as a functional marker for identification of LSECs (21,75). During some abnormal situations in which the hepatic scavenger system is either saturated or KCs are selectively depleted, LSECs may develop a limited phagocytic capacity, a phenomenon that is also observed during special in vitro conditions.…”

Section: Fenestrationmentioning

confidence: 99%

The liver sinusoidal endothelial cell: a cell type of controversial and confusing identity

Elvevold

Smedsrød

Martínez³

2008

American Journal of Physiology-Gastrointestinal and Liver Physi

215

212

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A look through the literature on liver sinusoidal endothelial cells (LSECs) reveals that there are several conflicts among different authors of what this cell type is and does. Major controversies that will be highlighted in this review include aspects of the physiological role, the characterization, and the protocols of isolation and cultivation of these cells. Many of these conflicts may be ascribed to the fact that the cell was only recently established as a distinct cell type and that researchers from different disciplines tend to define their structure and function differently. This field is in need of a common platform to obtain a sound communication and a unified understanding of how to interpret novel research results. The aim of this review is to encourage scientists not to ignore the fact that there are, indeed, different opinions in the literature on LSECs. We also hope that this review will point out to the reader that some issues that may seem well established regarding our knowledge about the LSECs, in reality, are still unresolved and, indeed, controversial.

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Large-Scale Isolation of Sinusoidal Endothelial Cells from Pig and Human Liver

Cited by 25 publications

References 31 publications

Lymphocyte traffic through sinusoidal endothelial cells is regulated by hepatocytes

Lymphocyte traffic through sinusoidal endothelial cells is regulated by hepatocytes

Hepatic sinusoidal endothelium avidly binds platelets in an integrin-dependent manner, leading to platelet and endothelial activation and leukocyte recruitment

The liver sinusoidal endothelial cell: a cell type of controversial and confusing identity

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