Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection.

Wang, Jingyi; Mushegian, Arcady; Lory, Stephen; Jin, Shouguang

doi:10.1073/pnas.93.19.10434

Cited by 164 publications

(157 citation statements)

References 34 publications

(20 reference statements)

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“…suis serotype 2 (Elliott & Tai, 1978), a role of the iri-7 gene in capsule biosynthesis can be proposed. iri-34 showed similarity to the np16 gene, previously identified using Environmentally regulated Streptococcus suis genes IVET selection in P. aeruginosa and suspected to encode threonine dehydratase activity (Wang et al, 1996). Together with the observation that four iri genes could be selected by the in vivo approach, these data suggest that a number of the iri genes may encode important virulence factors for Strep.…”

Section: Discussionsupporting

confidence: 53%

“…suis, genes. Three of these sequences (iri-2, iri-1, 6 and 22, and iri-34) were similar to sequences of environmentally regulated genes previously selected by applying the IVET to Vibrio cholerae (Camilli & Mekalanos, 1995), Staphylococcus aureus (Lowe et al, 1998) and Pseudomonas aeruginosa (Wang et al, 1996), respectively. One, contained in iri-1, 6 and 22, was similar to the agrA gene of Staph.…”

Section: Selection Of Promoters Induced Under Iron-restricted Conditionsmentioning

confidence: 65%

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Environmentally regulated genes of Streptococcus suis: identification by the use of iron-restricted conditions in vitro and by experimental infection of piglets The GenBank accession numbers for the sequences determined in this work are AF302190–AF302207 (iri genes) and AF303226–303247 (ivs genes).

Smith¹,

Buijs²,

Vries³

et al. 2001

Microbiology

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The identification of environmentally regulated genes of Streptococcus suis by the use of iron-restricted conditions in vitro and by experimental infection of piglets is described. Eighteen unique iron-restriction-induced (iri) genes and 22 unique in-vivo-selected (ivs) genes of Strep. suis were found. None of the ivs genes was exclusively expressed in vivo. Four iri genes were identical to four clones selected in piglets. Two ivs genes were similar to genes for putative virulence factors. One of these ivs genes was identical to the epf gene of virulent Strep. suis serotype 2 strains and the other showed homology to a gene encoding a fibronectin-binding protein of Streptococcus gordonii. Two additional ivs genes showed homology to environmentally regulated genes previously identified by using an in vivo expression technology (IVET) selection system in other bacterial species. One of these showed similarity to the agrA gene of Staphylococcus aureus, a key locus involved in the regulation of numerous virulence proteins. The promoter selection system described in this paper has been successfully used for the identification of many environmentally regulated genes potentially involved in the pathogenesis of Strep. suis infections in piglets.

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Section: Discussionsupporting

confidence: 53%

Section: Selection Of Promoters Induced Under Iron-restricted Conditionsmentioning

confidence: 65%

Environmentally regulated genes of Streptococcus suis: identification by the use of iron-restricted conditions in vitro and by experimental infection of piglets The GenBank accession numbers for the sequences determined in this work are AF302190–AF302207 (iri genes) and AF303226–303247 (ivs genes).

Smith¹,

Buijs²,

Vries³

et al. 2001

Microbiology

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“…Most of those genes were identified by whole genome sequencing and their functions are unknown. However, fur-like genes of Vibrio cholerae (39) and P. aeruginosa (40) were shown to be expressed, and the P. aeruginosa mutant does not have a fur-defective phenotype. From this, it is pertinent to ask whether bacteria other than B. japonicum contain Irr homologs that mediate iron control of heme synthesis.…”

Section: Discussionmentioning

confidence: 99%

The Bacterial Irr Protein Is Required for Coordination of Heme Biosynthesis with Iron Availability

Hamza

Chauhan²,

Hassett

et al. 1998

Journal of Biological Chemistry

150

198

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Heme is a ubiquitous macromolecule that serves as the active group of proteins involved in many cellular processes. The multienzyme pathway for heme formation culminates with the insertion of iron into a protoporphyrin ring. The cytotoxicity of porphyrins suggests the need for coordination of its biosynthesis with iron availability. We isolated a mutant strain of the bacterium Bradyrhizobium japonicum that, under iron limitation, accumulated protoporphyrin and showed aberrantly high expression of hemB, an iron-regulated gene encoding the heme synthesis enzyme ␦-aminolevulinic acid dehydratase. The strain carries a loss of function mutation in irr, a newly described gene that encodes a putative member of the GntR family of bacterial transcriptional regulators. Irr accumulated only under iron limitation, and turned over rapidly upon an increase in iron availability. A separate role for Irr in controlling the cellular iron level was inferred based on a deficiency in high affinity iron transport activity in the irr strain, and suggests that regulation of the heme pathway is coordinated with iron homeostasis. A high level of protoporphyrin accumulation is not a normal consequence of nutritional iron deprivation, thus a mechanism for iron-dependent control of heme biosynthesis may be present in other organisms.

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“…A Tn5G transposon insertional mutant library was generated as described earlier (Wang et al, 1996). Individual mutants were grown overnight in 96-well plates with L broth containing gentamicin.…”

mentioning

confidence: 99%

The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes

Ahn

Jia

et al. 2004

Microbiology

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Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection.

Abstract: ABSTRACT

Cited by 164 publications

References 34 publications

The Bacterial Irr Protein Is Required for Coordination of Heme Biosynthesis with Iron Availability

The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes

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