Large Scale Industrial Enzyme Production

Street, G.; Rodgers, Peter

doi:10.3109/07388558309082579

Cited by 12 publications

(2 citation statements)

References 61 publications

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“…AFB 1 -degrading enzymes have been purified from the fungi A. tabescens by ion-exchange chromatography and chromatofocusing chromatography [ 11 ], and from P. ostreatus by chromatography using DEAE-sepharose and phenyl sepharose columns [ 13 ]. In our study, BADE (40–50 mL, peak 2) with an estimated molecular mass of 22 kDa and the highest AFB 1 -degrading activity (3.85 × 10 3 U·mg −1 ) was purified 9.55-fold from B. shackletonii cultures with a 39.92% yield, implying a low processing cost [ 29 ] which, along with the reasonably high overall yield [ 30 ], makes it suitable for large-scale production.…”

Section: Discussionmentioning

confidence: 99%

Novel Aflatoxin-Degrading Enzyme from Bacillus shackletonii L7

Ahmed

Sangare

et al. 2017

Toxins

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Food and feed contamination by aflatoxin (AF)B1 has adverse economic and health consequences. AFB1 degradation by microorganisms or microbial enzymes provides a promising preventive measure. To this end, the present study tested 43 bacterial isolates collected from maize, rice, and soil samples for AFB1-reducing activity. The higher activity was detected in isolate L7, which was identified as Bacillus shackletonii. L7 reduced AFB1, AFB2, and AFM1 levels by 92.1%, 84.1%, and 90.4%, respectively, after 72 h at 37 °C. The L7 culture supernatant degraded more AFB1 than viable cells and cell extracts; and the degradation activity was reduced from 77.9% to 15.3% in the presence of proteinase K and sodium dodecyl sulphate. A thermostable enzyme purified from the boiled supernatant was designated as Bacillus aflatoxin-degrading enzyme (BADE). An overall 9.55-fold purification of BADE with a recovery of 39.92% and an activity of 3.85 × 103 U·mg−1 was obtained using chromatography on DEAE-Sepharose. BADE had an estimated molecular mass of 22 kDa and exhibited the highest activity at 70 °C and pH 8.0, which was enhanced by Cu2+ and inhibited by Zn2+, Mn2+, Mg2+, and Li+. BADE is the major protein involved in AFB1 detoxification. This is the first report of a BADE isolated from B. shackletonii, which has potential applications in the detoxification of aflatoxins during food and feed processing.

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Section: Discussionmentioning

confidence: 99%

Novel Aflatoxin-Degrading Enzyme from Bacillus shackletonii L7

Ahmed

Sangare

et al. 2017

Toxins

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“…In our study, MADE with an estimated molecular mass of 32 kDa by SDS‐PAGE was purified 166‐fold with a 57% yield by chromatography on DEAE‐Sepharose and gel filtration. Moreover, MADE from M. fu lvus is an extracellular enzyme that can be easily extracted and isolated, making the downstream processes less difficult with low processing cost (Ibrahim 2008) and high overall yield (Street and Rodgers 1983). Therefore, we can investigate the properties of extracellular enzymes more specifically and profoundly and prepare the recombinant enzymes at an industrial scale with modern genetic engineering methods.…”

Section: Discussionmentioning

confidence: 99%

Preparation, purification and characteristics of an aflatoxin degradation enzyme from Myxococcus fulvus ANSM068

Zhao

Guan

Gao

et al. 2010

Journal of Applied Microbiology

100

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Aims: To prepare, purify and characterize an extracellular enzyme from Myxococcus fulvus ANSM068, designated as myxobacteria aflatoxin degradation enzyme (MADE), which possesses degradation activity against aflatoxin B1 (AFB1), G1 (AFG1) and M1 (AFM1) in solution. Methods and Results: The culture supernatant of strain M. fulvus demonstrated high degradation ability against AFB1 (71·89%), AFG1 (68·13%) and AFM1 (63·82%) after 48 h of incubation. An enzyme was purified from the supernatant of M. fulvus using ethanol precipitation and chromatography on DEAE‐Sepharose and Superdex 75. An overall 166‐fold purification of the enzyme with a recovery of 57% and a final specific activity of 569·44 × 103 U mg−1 was obtained using the present purification protocol. The apparent molecular mass of MADE was estimated to be 32 kDa by SDS‐PAGE. AFG1 and AFM1 were significantly degraded, by 96·96 and 95·80%, respectively, when treated with pure MADE (100 U ml−1) produced by strain ANSM068. MADE exhibited the largest amount of activity at 35°C and pH 6·0, with Mg2+ ions greatly promoting and Zn2+ strongly inhibiting MADE activity. Conclusions: An aflatoxin degradation enzyme from bacterial isolates can effectively remove aflatoxin B1, G1 and M1 in solution. Significance and Impact of the Study: The high activity and wide temperature and pH range of MADE for the degradation of aflatoxin have promising applications in control of mycotoxins during food and feed processing.

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Large Scale Separation and Isolation of Proteins

Brocklebank¹

1987

Food Biotechnology—1

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Large Scale Industrial Enzyme Production

Cited by 12 publications

References 61 publications

Novel Aflatoxin-Degrading Enzyme from Bacillus shackletonii L7

Novel Aflatoxin-Degrading Enzyme from Bacillus shackletonii L7

Preparation, purification and characteristics of an aflatoxin degradation enzyme from Myxococcus fulvus ANSM068

Large Scale Separation and Isolation of Proteins

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