Large-scale gradient elution chromatography

Truei, Yung-Huoy; Gu, Tingyue; Tsai, Guo-Jane; Tsao, George T.

doi:10.1007/bfb0046196

Cited by 8 publications

(6 citation statements)

References 57 publications

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“…Gradient elution was performed as sharp peaks are obtained within short separation time [ 30 ]. The standard β-ODAP was not chemically modified and resolved into peak with retention time of about 7.4 min.…”

Section: Resultsmentioning

confidence: 99%

Simple Detection Methods for Antinutritive Factor β-ODAP Present in Lathyrus sativus L. by High Pressure Liquid Chromatography and Thin Layer Chromatography

et al. 2015

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Lathyrus sativus L. (Grass pea) is the source for cheap and nutritious food choice in drought and famine susceptible zones in greater part of North India and Africa. The non-protein amino acid β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP) has been known for decades for its potent neurotoxic effect, causing irreversible neurodegenerative disease “neurolathyrism”, present in both seed and leaf of Lathyrus sativus L. and other species in varying proportions. It is crucial to establish a rapid as well as reliable detection methodology for β-ODAP content in various Lathyrus plants. Currently available HPLC based methods involve multi-step derivatization of the sample. To overcome this, we have developed β-ODAP analysis method by HPLC without any prior derivatization. This method is statistically significant in the range of 2 to 100μg/ml and exhibited linear response with r 2 > 0.99. Limit of detection and quantitation of the later method was determined to be 5.56 μg/ml and 16.86 μg/ml, respectively. In addition to this, a TLC based method has also been developed. The limit of detection of β-ODAP is 0.6μg and for its substrate, L-1,2-diaminopropionic acid is 5μg. Both HPLC and TLC methods were validated by conducting in-vitro bioconversion test to detect the presence of biocatalyst in plant extract. This method is economical, rapid and simple.

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Section: Resultsmentioning

confidence: 99%

Simple Detection Methods for Antinutritive Factor β-ODAP Present in Lathyrus sativus L. by High Pressure Liquid Chromatography and Thin Layer Chromatography

et al. 2015

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“…Lipophilic species are mostly neutral with hydrophobic properties, owing to the alkyl groups . Chromatographic approaches, like ion-pairing reversed-phase, ion-exchange, ion-exclusion, and reversed-phase chromatographies, are reported to facilitate speciation of arsenicals in marine sample extracts, owing to variabilities in their physicochemical properties. , For example, at pH 7, MMA at p K a = 2.6 and DMA at p K a = 6.1 are anions and arsenocholine (AsC), tetramethylarsonium ion (TETRA), and trimethylarsine oxide (TMAO) at p K a = 3.6 are cations, while arsenobetaine (AsB) at p K a = 2.18 is zwitterionic. , Methylated arsenicals and AsSugars have been successfully isolated on anion-exchange (AE) columns, , while cation-exchange (CE) columns provide effective isolations for AsB, AsC, DMA, TMAO, TETRA, and DMAA. , AsLipids, however, require additional separation, using RP-LC typically with a C8 or C18 column. ,, …”

Section: Separationmentioning

confidence: 99%

“…Ordinarily, AE is performed in isocratic mode, mainly using aqueous mobile phases consisting of buffer salts with pH ranges covering neutral, basic, and acidic conditions . Gradient elution can shorten the analysis time without compromising resolutions of target analytes, allows for a broad range of retention, has a high peak capacity capable of handling a complex matrix, like seafood, and can overcome the general issues associated with elution. , Gradient elution protocols are rarely used because they cause baseline instabilities that could lead to inaccurate quantification and generally require extra instrument maintenance in comparison to isocratic elution. , …”

Section: Separationmentioning

confidence: 99%

Analytical Methodologies for the Determination of Organoarsenicals in Edible Marine Species: A Review

Luvonga

Rimmer

et al. 2020

J. Agric. Food Chem.

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Setting regulatory limits for arsenic in food is complicated, owing to the enormous diversity of arsenic metabolism in humans, lack of knowledge about the toxicity of these chemicals, and lack of accurate arsenic speciation data on foodstuffs. Identification and quantification of the toxic arsenic compounds are imperative to understanding the risk associated with exposure to arsenic from dietary intake, which, in turn, underscores the need for speciation analysis of the food. Arsenic speciation in seafood is challenging, owing to its existence in myriads of chemical forms and oxidation states. Interconversions occurring between chemical forms, matrix complexity, lack of standards and certified reference materials, and lack of widely accepted measurement protocols present additional challenges. This review covers the current analytical techniques for diverse arsenic species. The requirement for high-quality arsenic speciation data that is essential for establishing legislation and setting regulatory limits for arsenic in food is explored.

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“…1-2.4. 11.3 Evaluations of PeLi, 11i, and Bii 117 Truei et al [113] used some of the correlations above for parameter estimations in a case study involving gradient elution of four proteins in hydrophobic interaction chromatography. d is the molecular diameter of the solute.…”

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Mathematical Modeling and Scale-up of Liquid Chromatography

1995

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© Springer-Verlag Berlin Heidelberg 1995 Softcover reprin to the hardcover 1St edition 1995The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. PrefaceLiquid chromatography is no longer limited to chemical analysis. It has become an indispensable tool for the preparativeand large-scale purifications of proteins and other fine chemicals. So far, the scale-up of liquid chromatography relies mostly on trial and error and a few scaling rules that are more of a rule-of-thumb nature.This book provides numerical solutions to a series of general multicomponent rate models for liquid chromatography. The models consider dispersion, interfacial film mass transfer, intra particle diffusion, and nonlinear multicomponent isotherm, or the second order kinetics. The models can be used to simulate various chromatographic operations. They provide more realistic descriptions of preparative-and large-scale liquid chromatography than the equilibrium theory and plate models because various mass transfer mechanisms are included.The applications of the Fortran 77 codes for the models are explained. Parameter estimation for the models is discussed. The codes should be helpful in both the understanding of the dynamics of liquid chromatography and its scale-up. The codes are available to readers upon request by a letter, or preferably an electronic mail (to guting@ent.ohiou.edu).

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Large-scale gradient elution chromatography

Cited by 8 publications

References 57 publications

Simple Detection Methods for Antinutritive Factor β-ODAP Present in Lathyrus sativus L. by High Pressure Liquid Chromatography and Thin Layer Chromatography

Simple Detection Methods for Antinutritive Factor β-ODAP Present in Lathyrus sativus L. by High Pressure Liquid Chromatography and Thin Layer Chromatography

Analytical Methodologies for the Determination of Organoarsenicals in Edible Marine Species: A Review

Mathematical Modeling and Scale-up of Liquid Chromatography

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