Large-scale DNA fingerprinting of Mycobacterium tuberculosis strains as a tool for epidemiological studies of tuberculosis

Chevrel-Dellagi, D.; Abderrahman, Amel; Haltiti, Raja; Koubaji, Hichem; Gicquel, Brigitte; Dellagi, Koussay

doi:10.1128/jcm.31.9.2446-2450.1993

Cited by 67 publications

(24 citation statements)

References 14 publications

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“…By use of IS6110 DNA fingerprinting the degree of DNA polymorphism of M. tuberculosis strains isolated in Dar es Salaam was found to be similar to that recently observed in New York and San Francisco (1,23) because the average number of isolates per pattern was 1.33 for the present study, whereas for the study in New York it was 1.35 and for the study in San Francisco it was 1.5. It might be expected that regions suffering from a high level of TB transmission and insufficient In contrast to recent observations from a large-scale RFLP study of M. tuberculosis isolates in Tunisia (6) and an ongoing study in Denmark (31), the number of IS6110 copies observed in the isolates investigated in the present study was rather evenly distributed within a range of from 1 to 20. In the study in Tunisia, about 75% of the investigated isolates of M. tuberculosis were found to carry from 6 to 10 copies of the IS6110 element.…”

Section: Discussioncontrasting

confidence: 99%

“…The low rate of primary drug resistance observed in the present study showed that, despite the substantial difficulties in Tanzania, as in all other developing countries, the national TB control program in Tanzania has proved to be quite efficient. In accordance with previous findings (6,26), there was no correlation between DNA fingerprint patterns and drug resistance. However, phenotypic characteristics which classify M. tuberculosis strains as being of the Asian subgroup correlated with a low IS6110 copy number per isolate.…”

Section: Discussionsupporting

confidence: 92%

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DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HIV)-seropositive and HIV-seronegative patients in Tanzania

et al. 1995

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With the purpose of determining whether the risk of infection with a particular clone of Mycobacterium tuberculosis is influenced by the human immunodeficiency virus (HIV) status of the host, we analyzed and compared 68 mycobacterial isolates obtained from HIV-seropositive patients with tuberculosis (TB) in Dar esSalaam, Tanzania, with 66 mycobacterial isolates obtained from HIV-seronegative patients with TB in the same geographical region by using both DNA fingerprinting and classical phenotyping methods. One hundred one different IS6110 fingerprinting patterns were observed in the 134 isolates. The level of diversity of the DNA fingerprints observed in the HIV-seropositive group was comparable to the level of the diversity observed in the HIV-seronegative group. Resistance to a single anti-TB drug was found in 8.8% of the tested isolates, and 3.2% of the isolates were resistant to more than one anti-TB drug. The drug susceptibility profiles were not significantly difference between the two groups of isolates compared in the present study. Phenotypic characteristics which classify M. tuberculosis strains as belonging to the Asian subgroup correlated with a low IS6110 copy number per isolate. However, the occurrence of Asian subgroup strains was not associated with the HIV status of the patients. The results of the study suggested an equal risk of infection with a defined M. tuberculosis clone for HIV-seropositive and HIV-seronegative individuals.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 92%

DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HIV)-seropositive and HIV-seronegative patients in Tanzania

et al. 1995

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“…In this cohort analysis we have found a large degree of IS6110 polymorphisms among the strains. The number of copies ranged from 1 to 16 elements (1 to 6 copies in 19.2% of the isolates, 7 to 11 copies in 65.4% of the isolates, and 12 to 16 in 15.4% of the isolates), similar to what has been reported previously (6,33). All strains had been identified as M. tuberculosis, including two (isolates 19 and 20) exhibiting only a single copy of IS6110 (verified by their strongly positive nitrate and niacin reactions, the aerophilic growth characteristics on Lebek medium, and their resistance to thiophene-2-carboxylic acid hydrazide which thus rules out their identity as Mycobacterium bovis [15]).…”

Section: Discussionsupporting

confidence: 86%

“…Restriction fragment length polymorphism (RFLP) typing of Mycobacterium tuberculosis has become a powerful tool for differentiating strains with a high degree of confidentiality and circumvents the significant limitations of other systems which are based on phages or antibiotic susceptibility patterns (6,31). The level of genetic relatedness of M. tuberculosis strains can easily be visualized by probing chromosomal DNA of tubercle bacilli with genetic markers which result in distinct RFLP patterns.…”

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confidence: 99%

Molecular epidemiology of Mycobacterium tuberculosis strains isolated from patients in a human immunodeficiency virus cohort in Switzerland

et al. 1997

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From 1989 to 1995, 46 patients infected with the human immunodeficiency virus were diagnosed with tuberculosis at the University Hospital in Zurich. Using the IS6110 insertion sequence as a genetic marker, restriction fragment length polymorphism analyses were done for 52 Mycobacterium tuberculosis isolates. We have found a large degree of IS6110 polymorphism, ranging from 1 to 16 copies. For isolates from patients from whom multiple isolates had been available, the IS6110 pattern remained virtually stable over a period of up to 4 years, as well as during emerging drug resistance. In none of the cases was a reinfection of a patient with another strain detected. For isolates from 10 patients we detected identical patterns which could be associated with four clusters. In one of these, the strains exhibited a low IS6110 copy number (four bands), and the strains were further analyzed by hybridizing with (i) the polymorphic GC-rich repetitive sequence (PGRS) and (ii) the 36-bp direct-repeat (DR) cluster sequence. One of these isolates had a different pattern with the PGRS as well as with the DR sequence and could therefore be safely excluded from that cluster. These findings point to the importance of applying more than one genetic criterion in the molecular biological study of strain relatedness.

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“…Molecular clusters defined by the applied genotyping methods are a surrogate marker for recent transmission and epidemiological linked cases [23]. The high clustering rate of 63.8% in this study indeed indicates that the majority of active TB cases in Eastern Ethiopia are due to recent transmission and not reactivation.…”

Section: Discussionmentioning

confidence: 70%

Molecular epidemiology and drug resistance patterns ofMycobacterium tuberculosiscomplex isolates from university students and the local community in Eastern Ethiopia

Mekonnen

Merker

Collins

et al. 2018

Preprint

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BackgroundPrevious studies suggest the burden of pulmonary tuberculosis (PTB) in Ethiopia may be greater in university students relative to the overall population. However, little is known about the transmission dynamics of PTB among students and members of the communities surrounding university campuses in Eastern Ethiopia.MethodsA cross sectional study was conducted in Eastern Ethiopia among culture-confirmed PTB cases from university students (n=36) and community members diagnosed at one of four hospitals (n=152) serving the surrounding area. Drug susceptibility testing (DST) was performed on Mycobacterium Tuberculosis Complex (MTBC) isolates using BD Bactec MGIT 960 and molecular genotyping was performed using spoligotyping and 24-loci MIRU-VNTR. MTBC strains with Identical genotyping patterns were assigned to molecular clusters as surrogate marker for recent transmission and further contact tracing was initiated among clustered patients.ResultsAmong all study participants, four MTBC lineages and 11 sub-lineages were identified, with Ethiopia_3 being most common sub-lineage (29.4%) and associated with strain clustering (P= 0.016). We identified 13 (8.1%) strains phylogenetically related to the known Ethiopian sub-lineages with a distinct Spoligotyping patterns and designated as Ethiopia_4. The clustering rate of MTB strains was 52.9% for university students and 66.7% for community members with a Recent Transmission Index (RTI) of 17.6% and 48.4%, respectively. Female gender, urban residence, and new TB cases were significantly associated with strain clustering (p<0.05). Forty-eight (30%) of the study participants were resistant to one or more first line anti TB drugs, three patients were classified as multidrug resistant (MDR), defined by isoniazid and rifampicin resistance.ConclusionWe found evidence of significant PTB cases clustering and recent transmission among Ethiopian university students and the local community in eastern Ethiopia; with Ethiopia_3 being the predominant circulating sub-lineage. A country wide comprehensive molecular surveillance and drug resistance profiling of MTBC strains and Implementation of TB control programs within universities and the surrounding community should be considered to decrease TB transmission.

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Large-scale DNA fingerprinting of Mycobacterium tuberculosis strains as a tool for epidemiological studies of tuberculosis

Cited by 67 publications

References 14 publications

DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HIV)-seropositive and HIV-seronegative patients in Tanzania

DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HIV)-seropositive and HIV-seronegative patients in Tanzania

Molecular epidemiology of Mycobacterium tuberculosis strains isolated from patients in a human immunodeficiency virus cohort in Switzerland

Molecular epidemiology and drug resistance patterns ofMycobacterium tuberculosiscomplex isolates from university students and the local community in Eastern Ethiopia

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