Large-Scale Detection of Metals with a Small Set of Fluorescent DNA-Like Chemosensors

Yuen, Lik Hang; Franzini, Raphael M.; Tan, Siyuan; Kool, Eric T.

doi:10.1021/ja507932a

Cited by 57 publications

(53 citation statements)

References 25 publications

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“…3 Most DNA-based devices fall broadly into two classes based on their mechanisms of action- first, Pb 2+ -dependent DNAzyme-catalyzed RNA cleavage, 4a-f and second, Pb 2+ -dependent formation of DNA G-quadruplex (Table S1 † ). 3,5a-c,6a,b In the first class of sensors, the rates of signal enhancement need to be monitored for quantitative detection, requiring substantial data processing. 4a-f The second class exploits the potential of Pb 2+ to induce formation of G-quadruplexes from single or double-stranded G-rich DNA.…”

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confidence: 99%

“…4a-f The second class exploits the potential of Pb 2+ to induce formation of G-quadruplexes from single or double-stranded G-rich DNA. This class of sensors usually needs covalent incorporation of fluorophores 6a,b or involves chemical reactions that generate colored/chemiluminiscent products, 3,5a-c which limit the use of these sensors to certain favourable conditions of pH and ionic strength. DNA based electrochemical sensors, most of which use one of the above mechanisms, have been used for sensitive Pb 2+ detection.…”

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confidence: 99%

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Spinach RNA aptamer detects lead(ii) with high selectivity

DasGupta

Shelke

et al. 2015

Chem. Commun.

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Spinach RNA aptamer contains a G-quadruplex motif that serves as a platform for binding and fluorescence activation of a GFP-like fluorophore. Here we show that Pb2+ induces formation of Spinach’s G-quadruplex and activates fluorescence with high selectivity and sensitivity. This device establishes the first example of an RNA-based sensor that provides a simple and inexpensive tool for Pb2+ detection.

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confidence: 99%

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confidence: 99%

Spinach RNA aptamer detects lead(ii) with high selectivity

DasGupta

Shelke

et al. 2015

Chem. Commun.

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“…With these properties, we aim to prepare a sensor array for pattern-recognition-based detection. Compared with other array strategies for Ln 3+ discrimination, 8 the choice of the sensor molecules here is more targeted since they were selected based on their specific activity, which may facilitate the distinction.…”

Section: Resultsmentioning

confidence: 99%

“…[8][9][10][11][12][13] DNA contains a phosphate backbone that affords high Ln 3+ binding affinity, while the nitrogen containing nucleobases might help discriminate individual Ln 3+ . 7 Ln 3+ forms complexes with nucleotides, especially with adenosine and guanosine phosphates.…”

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Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array

et al. 2016

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Developing chemical probes to distinguish each lanthanide ion is a long-standing challenge. Aside from its analytical applications, solving this problem will also enhance our knowledge in metal ligand design. Using in vitro selection, we previously reported four RNA-cleaving DNAzymes, each with a different activity trend cross the lanthanide series. We herein performed another eight in vitro selection experiments using each and every lanthanide from La 3+ to Tb 3+ but excluding the radioactive Pm 3+ . A new DNAzyme named Gd2b was identified and characterized. By labeling this DNAzyme with a fluorophore/quencher pair to create a catalytic beacon, a detection limit of 14 nM Gd 3+ was achieved. With the same beacon design, all the five lanthanide-specific DNAzymes were used together to form a sensor array. Each lanthanide ion produces a unique response pattern with these five sensors, allowing a pattern-recognition-based linear discriminant analysis (LDA) algorithm to be applied, where separation was achieved between lanthanides and nonlanthanides, light and heavy lanthanides, and for the most part, each lanthanide. These lanthanidespecific DNA molecules are useful for understanding lanthanide coordination chemistry, designing hybrid materials, and developing related analytical probes.

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“…Limited success was achieved in sensing lanthanides with chemical or biological probes, [43][44][45][46] and the reported probes often suffer from low sensitivity or specificity. This can be attributed to the similar chemical properties of all of the lanthanides.…”

Section: Sensing Lanthanidesmentioning

confidence: 99%

Lanthanide-dependent RNA-cleaving DNAzymes as metal biosensors

Liu

2015

Can. J. Chem.

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Lanthanides represent a group of very important but challenging analytes for biosensor development. These 15 elements are very similar in their chemical properties. So far, limited success has been realized using the rational ligand design approach. My laboratory has successfully accomplished the task of carrying out combinatorial selection to isolate lanthanide-dependent RNA-cleaving DNAzymes. We report two new DNAzymes, each discovered in a different selection condition and both are highly specific to lanthanides. When both DNAzymes are used together, it is possible to identify the last few heavy lanthanides. Upon introducing a phosphorothioate modification, one of the abovementioned DNAzymes becomes highly active with many toxic heavy metals. With the selection of more DNAzymes with different activity patterns cross the lanthanide series, a sensor array might be produced for identifying each ion. This article is a minireview of the current developments on this topic and some of the historical aspects. It reflects the main content of the Fred Beamish Award presentation delivered at the 2014 Canadian Society for Chemistry Conference in Vancouver. Future directions in this area are also discussed.Key words: lanthanides, DNAzymes, biosensors, fluorescence, metal ions.Résumé : Les lanthanides représentent un groupe d'analytes très important, mais aussi très complexe en ce qui concerne la mise au point de biocapteurs. Ces 15 éléments sont très similaires quant à leurs propriétés chimiques. Jusqu'à présent, le succès de la conception rationnelle de ligands s'est révélé limité. Mon laboratoire est parvenu avec succès à effectuer une sélection combinatoire afin d'isoler des ADNzymes de clivage d'ARN dont l'action dépend des lanthanides. Nous présentons deux nouvelles ADNzymes, dont chacune a été découverte dans des conditions de sélection différentes et qui sont toutes deux hautement spécifiques aux lanthanides. Lorsque les deux ADNzymes sont utilisées ensemble, il est possible d'identifier les quelques lanthanides les plus lourds. En apportant une modification par l'ajout de phosphorothioate, l'une des ADNzymes mentionnées plus haut devient hautement active en présence de plusieurs métaux lourds toxiques. Grâce à la sélection d'autres ADNzymes présentant différents profils d'activité en présence des divers ions de la série des lanthanides, un réseau de capteurs pourrait être produit pour permettre l'identification de chaque ion. Le présent article constitue une mini-synthèse des progrès actuels réalisés sur ce sujet et de certains des aspects historiques. Il reflète dans les grandes lignes le contenu de la présentation du prix Fred Beamish prononcée dans le cadre du congrès de la Société canadienne de chimie qui s'est tenu à Vancouver en 2014. Les orientations futures dans ce secteur de recherche sont également abordées. [Traduit par la Rédaction]

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Large-Scale Detection of Metals with a Small Set of Fluorescent DNA-Like Chemosensors

Cited by 57 publications

References 25 publications

Spinach RNA aptamer detects lead(ii) with high selectivity

Spinach RNA aptamer detects lead(ii) with high selectivity

Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array

Lanthanide-dependent RNA-cleaving DNAzymes as metal biosensors

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