Large scale biobanking of blood — The importance of high density sample processing procedures

Malm, Johan; Végvári, Ákos; Rezeli, Melinda; Upton, Paul; Danmyr, Pia; Nilsson, R; Steinfelder, Erik; Markó-Varga, György

doi:10.1016/j.jprot.2012.05.003

Cited by 28 publications

(22 citation statements)

References 14 publications

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“…Multiple aliquoting is advised to reduce future freeze-thaw cycles. A recent study confirmed the validity and reliability of a highthroughput, high-density, low-volume biobank sample processing solution for blood fractionation and archiving biospecimens that used the 384 aliquoting format sample storage tube system (86 ). A study of high-density scaling See text for detailed references.…”

Section: Reviewmentioning

confidence: 89%

“…Multiple aliquots should be created at the beginning of processing a biospecimen rather than delayed until the specific assay is conducted, because repeated freeze-thaw cycles may be detrimental in some cases (e.g., RNA) (7,86 ). If the collection site is not close to the laboratory, it may be appropriate to perform simple processes such as on-site centrifugation, aliquoting, fractionation of serum, and isolation of buffy coat and plasma, and to store the samples in smaller transportable coolers or freezers.…”

Section: Reviewmentioning

confidence: 99%

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Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Ellervik

Vaught²

2015

Clinical Chemistry

137

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BACKGROUND Most errors in a clinical chemistry laboratory are due to preanalytical errors. Preanalytical variability of biospecimens can have significant effects on downstream analyses, and controlling such variables is therefore fundamental for the future use of biospecimens in personalized medicine for diagnostic or prognostic purposes. CONTENT The focus of this review is to examine the preanalytical variables that affect human biospecimen integrity in biobanking, with a special focus on blood, saliva, and urine. Cost efficiency is discussed in relation to these issues. SUMMARY The quality of a study will depend on the integrity of the biospecimens. Preanalytical preparations should be planned with consideration of the effect on downstream analyses. Currently such preanalytical variables are not routinely documented in the biospecimen research literature. Future studies using biobanked biospecimens should describe in detail the preanalytical handling of biospecimens and analyze and interpret the results with regard to the effects of these variables.

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Section: Reviewmentioning

confidence: 89%

Section: Reviewmentioning

confidence: 99%

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Ellervik

Vaught²

2015

Clinical Chemistry

137

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“…We recently presented a 384 high density solution [17] that is currently being implemented as an efficient and cheap solution to Biobank archiving in clinical hospitals. The 384-rack is confined with a hard polymeric frame where the tubes (384) are mounted.…”

Section: Resultsmentioning

confidence: 99%

“…The reason for this strategy is to maintain the sample quality over time, which is impossible if the sample goes through repetitive freezethaw cycles. By utilizing high density 384-tube formats, with 70 μL sample volumes, the single use approach can be fulfilled [17]. In order to maintain the sample integrity and quality over time, the principle of single usage is also gaining acceptance.…”

Section: Robotic Sample Processingmentioning

confidence: 99%

“…lifegene.se/). Keeping an absolute control of patient sampling, standardized procedures, validated sample handling protocols and electronic surveillance of the sample life cycle ranging from the needle in the arm of the patient to the automated storage handling at −80°C will provide the basis for high quality biobanks [2,14,17]. New technology is changing a lot of the way that standard procedure in hospitals is performed.…”

Section: Introductionmentioning

confidence: 99%

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Standardization developments for large scale biobanks in smoking related diseases - a model system for blood sample processing and storage

et al. 2013

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Background: Biobank samples stored in biobanks give researchers and respiratory healthcare institutions access to datasets of analytes valuable for both diagnostic and research practices. The usefulness of these samples in clinical decision-making is highly dependent on their quality and integrity. New procedures that better preserve sample integrity and reduce degradation are being developed to meet the needs of both present and future biobanking. Hereby we present an automatic sample workflow scheme that is designed to handle high numbers of blood samples.

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The Human Melanoma Proteome Atlas—Complementing the melanoma transcriptome

Betancourt

Gil

Sánchez

et al. 2021

Clinical & Translational Med

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Large scale biobanking of blood — The importance of high density sample processing procedures

Cited by 28 publications

References 14 publications

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Standardization developments for large scale biobanks in smoking related diseases - a model system for blood sample processing and storage

The Human Melanoma Proteome Atlas—Complementing the melanoma transcriptome

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