Large RNase T1-resistant oligonucleotides encoding p15E and the U3 region of the long terminal repeat distinguish two biological classes of mink cell focus-forming type C viruses of inbred mice

Lung, ML; Hartley, Janet W.; Rowe, Wallace P.; Hopkins, Nancy

doi:10.1128/jvi.45.1.275-290.1983

“…Mink cell focus-forming (MCF) retroviruses have been implicated as the proximal leukemogenic agents in the induction of spontaneous leukemia in certain inbred strains of mice (19,21,23,34). MCF viruses have been isolated from the thymus and other tissues of AKR mice during the preleukemic period (9, 10,15,16).…”

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confidence: 99%

“…MCF viruses have been isolated from the thymus and other tissues of AKR mice during the preleukemic period (9, 10,15,16). Some MCF isolates when injected into newborn animals accelerate the onset of leukemia (10,34). The ability to replicate to high titers in the thymus and express high levels of murine leukemia virus (MuLV)-related cell surface antigens on infected thymocytes is tightly correlated with the oncogenic phenotype of this class of MCF viruses (20,28,36,37).…”

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confidence: 99%

“…The ability to replicate to high titers in the thymus and express high levels of murine leukemia virus (MuLV)-related cell surface antigens on infected thymocytes is tightly correlated with the oncogenic phenotype of this class of MCF viruses (20,28,36,37). MCF 247 is the prototype of thymotropic, oncogenic MCF viral isolates (23)(24)(25)34).…”

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confidence: 99%

“…In some cases, the structures of the recombinants were confirmed by analyzing RNase T1 fingerprints of viruses produced after transfection of the recombinants. The fingerprints were analyzed for the presence or absence of the unique MCF 247 or MCF 30-2 RNase Tl-resistant oligonucleotides in the precursor to the piSE gene (PrplSE gene) (29,34).…”

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confidence: 99%

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Influence of enhancer sequences on thymotropism and leukemogenicity of mink cell focus-forming viruses

Holland

¹

,

Thomas

²

,

Chattopadhyay

³

et al. 1989

J Virol

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Oncogenic mink cell focus-forming (MCF) viruses, such as MCF 247, show a positive correlation between the ability to replicate efficiently in the thymus and a leukemogenic phenotype. Other MCF viruses, such as MCF 30-2, replicate to high titers in thymocytes and do not accelerate the onset of leukemia. We used these two MCF viruses with different biological phenotypes to distinguish the effect of specific viral genes and genetic determinants on thymotropism and leukemogenicity. Our goal was to identify the viral sequences that distinguish thymotropic, nonleukemogenic viruses such as MCF 30-2 from thymotropic, leukemogenic viruses such as MCF 247. We cloned MCF 30-2, compared the genetic hallmarks of MCF 30-2 with those of MCF 247, constructed a series of recombinants, and tested the ability of the recombinant viruses to replicate in the thymus and to induce leukemia. The results established that (i) MCF 30-2 and MCF 247 differ in the numbers of copies of the enhancer sequences in the long terminal repeats. (ii) The thymotropic phenotype of both viruses is independent of the number of copies of the enhancer sequences. (iii) The oncogenic phenotype of MCF 247 is correlated with the presence in the virus of duplicated enhancer sequences or with the presence of an enhancer with a specific sequence. These results show that the pathogenic phenotypes of MCF viruses are dissociable from the thymotropic phenotype and depend, at least in part, upon the enhancer sequences. On the basis of these results, we suggest that the molecular mechanisms by which the enhancer sequences determine thymotropism are different from those that determine oncogenicity.

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“…The animals from which the mouse tumor cells are derived express the ecotropic AKV virus in all tissues, and multiple copies of the ''mink cell focus-inducing'' (MCF) provirus (Hartley et al, 1977;O'Donnell et al, 1981) are found integrated in the genomes of the leukemic cells (Herr and Gilbert, 1983). The MCF virus is a result of recombination between germline ecotropic (mouse-infectious) AKV and nonecotropic murine leukemia virus (MuLV) (Lung et al, 1983). A search through the genomes of AKV and MCF shows a number of potential epitopes that fit the RT1.A1 1 and RT1.A2 1 motifs (Tables IB, IIB).…”

Section: Discussionmentioning

confidence: 99%

Multiple class I motifs revealed by sequencing naturally processed peptides eluted from rat T cell MHC molecules

Burrows

¹

,

Ariail

²

,

Celnik

³

et al. 1997

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Class I major histocompatibility complex (MHC) molecules interact with a diverse array of self and foreign peptides. Displayed on the cell surface, the class I/peptide complex provides an extracellular indication of the intracellular milieu. We have characterized the Lewis rat Vbeta8.2+ T cell hybridoma C14/BW12-12A1 by FACS analysis and have used immunoaffinity chromatography to purify class I molecules from these cells. Peptides eluted from the class I molecules have been fractionated by HPLC and sequenced. Self-peptide mixtures indicate two distinct peptide motifs, suggesting the possibility of multiple class I loci. The majority of the naturally processed peptide ligands were nonamers. Naturally processed peptide ligands fitting the first motif contained a hydrophobic leucine anchor residue at position three and a carboxyl-terminal serine anchor residue. A second motif was characterized by a tyrosine or phenylalanine residue at position three and a phenylalanine or isoleucine carboxyl-terminal residue. Four peptides derived from the Vbeta8.2 T cell receptor have sequences that fit these motifs, providing a mechanistic explanation for their immunoregulatory role. Identification of these class I peptide binding motifs will be useful for predicting potential CTL epitopes in studies on autoimmunity, immunoregulation and transplantation in the Lewis rat.

show abstract

Multiple class I motifs revealed by sequencing naturally processed peptides eluted from rat T cell MHC molecules

Burrows

¹

,

Ariail

²

,

Celnik

³

et al. 1997

View full text Add to dashboard Cite

Class I major histocompatibility complex (MHC) molecules interact with a diverse array of self and foreign peptides. Displayed on the cell surface, the class I/peptide complex provides an extracellular indication of the intracellular milieu. We have characterized the Lewis rat Vbeta8.2+ T cell hybridoma C14/BW12-12A1 by FACS analysis and have used immunoaffinity chromatography to purify class I molecules from these cells. Peptides eluted from the class I molecules have been fractionated by HPLC and sequenced. Self-peptide mixtures indicate two distinct peptide motifs, suggesting the possibility of multiple class I loci. The majority of the naturally processed peptide ligands were nonamers. Naturally processed peptide ligands fitting the first motif contained a hydrophobic leucine anchor residue at position three and a carboxyl-terminal serine anchor residue. A second motif was characterized by a tyrosine or phenylalanine residue at position three and a phenylalanine or isoleucine carboxyl-terminal residue. Four peptides derived from the Vbeta8.2 T cell receptor have sequences that fit these motifs, providing a mechanistic explanation for their immunoregulatory role. Identification of these class I peptide binding motifs will be useful for predicting potential CTL epitopes in studies on autoimmunity, immunoregulation and transplantation in the Lewis rat.

show abstract

Large RNase T1-resistant oligonucleotides encoding p15E and the U3 region of the long terminal repeat distinguish two biological classes of mink cell focus-forming type C viruses of inbred mice

Cited by 97 publications

References 39 publications

Influence of enhancer sequences on thymotropism and leukemogenicity of mink cell focus-forming viruses

Influence of enhancer sequences on thymotropism and leukemogenicity of mink cell focus-forming viruses

Multiple class I motifs revealed by sequencing naturally processed peptides eluted from rat T cell MHC molecules

Multiple class I motifs revealed by sequencing naturally processed peptides eluted from rat T cell MHC molecules

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