Large plasmids in an actinomycete

Dobritsa, Svetlana V.

doi:10.1111/j.1574-6968.1984.tb01031.x

Cited by 13 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strains of another group constitute a separate taxospecies (Evtushenko et al, in press) and exhibit high degrees of DNA relatedness (67 to 94%), corresponding to a single genospecies. The close genetic relatedness among some strains of the latter species was demonstrated by the identical or similar banding pat-terns of their genomic DNA restriction fragments (10,11) and by the same plasmid composition (8). The members of this species have low levels of DNA homology with all Amycolata species described previously, cluster at some distance by numerical taxonomic analysis (Evtushenko et al, in press), and display a number of phenotypic characteristics which differentiate them from Amycolata autotrophica , Amycolata saturnea, and Amycolata hydrocarbonoxydans.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

A New Species of Actinomycete, Amycolata alni

Evtushenko

Акимов

Dobritsa

et al. 1989

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

A new species of the genus Amycoluta, Amycolata alni, is proposed for strains which were mostly isolated from root nodules and rhizospheres of alder trees and were formerly assigned to the species Amycoluta uutotrophicu. Strains of this new species could be differentiated from representatives of A. uutotrophica by deoxyribonucleic acid homology data (17 to 29% relatedness), as well as by their ability to grow on salicin and D-gluconate, their failure to produce acid from cellobiose and meso-inositol, their resistance to penicillin and carbenicillin (10 pg/ml), and other characteristics. Phenotypic features and low levels of deoxyribonucleic acid homology distinguish A. alni from two other species of the genus Amycolatu, Amycoluta suturneu and Amycolata hydrocuibonoxydans. The type strain of A. alni is strain 3LS (= VKM Ac-901).Eleven phenotypically similar nocardioform actinomycetes having a type IV cell wall composition and lacking mycolic acids were isolated from nitrogen-fixing root nodules and rhizospheres of two alder species, Alnus glutinosa and Alnus incana (29; L. S. Sharaya, Diss. C. Sci. thesis, Institute of Biochemistry and Physiology of Microorganisms, Pushchino, USSR, 1985). According to the identification keys and species descriptions that were available at that time (3,14,15), these isolates were assigned to the species Nocardia autotrophica (20; Y. B. Malishkaite, Diss. C. Sci. thesis, University of Moscow, Moscow, USSR, 1984). Recently, N . autotrophica was included as the type species in the genus Amycolata, which was proposed, along with the genus Amycolatopsis, to accommodate nocardioform actinomycetes having cell wall chemotype IV and lacking mycolic acids (19). Besides the type strain, strain ATCC 19727, the description of Amycolata autotrophica included 28 strains of various origins assigned to N . autotrophica by Gordon et al. (14). Many physiological characteristics exhibited by these 28 strains are variable or different from those of the type strain (19), thus indicating that the species Amycolata autotrophica may be a heterogeneous taxon.Variations in deoxyribonucleic acid (DNA) guanine-pluscytosine (G+C) ratios (60 to 72 mol%) and DNA homology values (37 to 67%) (24) among five strains from the group studied by Gordon et al. (14) have shown that strains of different genospecies may have been placed in Amycolata autotrophica primarily on the basis of physiological criteria.Recently , genetic heterogeneity among strains assigned to Amycolata autotrophica, including the strains from alder associations, was demonstrated by restriction fragment analysis of total genomic DNAs (10). These data showed that the alder isolates are distantly related to the type strain of Amycolata autotrophica.Because of the obvious differences in genome structure among strains of the same species, as well as the abovementioned physiological variations, we compared strains assigned to Am.ycolata autotrophica more extensively. On the basis of DNA-DNA hybridization data and chemotaxonomic and physiological charac...

show abstract

Section: Discussionmentioning

confidence: 98%

“…thesis). Among them, strain 3LS (= VKM Ac-901) is the best-studied organism (1,7,8,10,11,18,20,29; Malishkaite, Diss. C. Sci.…”

Section: Discussionmentioning

confidence: 99%

A New Species of Actinomycete, Amycolata alni

Evtushenko

Акимов

Dobritsa

et al. 1989

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

show abstract

“…The procedure described by Dobritsa (14), with slight modification (52), was used. Cultures were grown on modified Prauser medium 79 at 28°C for 44 h. A 1-ml portion of a culture suspension was harvested in a 1.5-ml microcentrifuge tube by centrifugation, washed once with 300 pl of a solution containing 10% sucrose in 0.05 M Tris-HC1 (pH 8.0), and resuspended in 300 p1 of the same solution supplemented with 15 ~1.1 of 0.5 M disodium EDTA (pH 8.0) and lysozyme (Sigma) (100 mg/ml in TE buffer containing 0.05 M Tris-HC1 and 0.02 M disodium EDTA [pH 8.01) to a final concentration of 10 mg/ml.…”

Section: Methodsmentioning

confidence: 99%

Rathayibacter gen. nov., Including the Species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and Six Strains from Annual Grasses

Zgurskaya¹,

Evtushenko²,

Акимов³

et al. 1993

International Journal of Systematic Bacteriology

125

View full text Add to dashboard Cite

A new genus, Ruthuyibucter, is proposed to accommodate three species of gram-positive, aerobic, coryneform bacteria previously placed in the genus Clavibucter (Ruthuyibucter ruthuyi comb. nov., Ruthuyibucter tritici comb. nov., and Ruthuyibucter irunicus comb. nov.), as well as six strains that were isolated from annual cereal grasses, may be responsible for ryegrass toxicity, and are very similar to the recently described organism Chvibacter toxicus sp. nov. (I. T. Riley and K. M. Ophel, Int. J. Syst. Bacteriol. 42:64-68, 1992). The properties of members of the genus Ruthuyibacter include coryneform morphology, peptidoglycan based on 2,4-diaminobutyric acid (type B2y), predominant menaquinones of the MK-10 type, and phosphatidylglycerol and diphosphatidylglycerol as basic polar lipids. The DNA base compositions range from 63 to 72 mol% G+C. The members of the new genus form a phenetic cluster distinct from Clavibucter spp. at a level of 71% (simple matching coefficient) and exhibit 7 to 9% DNA-DNA reassociation with strains of Chvibucter spp. In contrast to Clavibacter spp., most Ruthuyibucter strains are associated with nematodes belonging to the genus Anguina, The Ruthayibucter species differ from species belonging to related genera (e.g., Chvibucter and Agromyces species) in the following characteristics: menaquinone and whole-cell sugar compositions, results of lysozymesodium dodecyl sulfate test (which indicates differences in cell wall composition), ability to utilize a number carbon sources, resistance to bacteriocins of some Chvibucter spp., and other characteristics. The Ruthayibucter species can be differentiated from each other by following characteristics: presence or absence of xylose and galactose in the cell walls, fatty acid composition, ability to assimilate various sources of carbon and nitrogen, hydrolytic activity, tolerance to 5% NaCl and 0.03% potassium tellurite, susceptibility to iranicin, and absence or presence of plasmids of certain sizes. The type species of the genus Ruthuyibucter is R. ruthuyi. The type strains of the species are R. ruthuyi VKM Ac-1601 (= ICMP 2574), R. irunicus VKM Ac-1602 (= ICMP 3496), and R. tritici VKM Ac-1603 (= ICMP 2626).

show abstract

“…Plasmid A nalysis Plasmid DNA was extracted from each strain using the method of Dobritsa [9] which is a modification of Kado and Liu's procedure [10]. All isolates revealed the presence of only the 2.6-megadalton plasmid.…”

Section: Further Examination O F Gonococcal Isolatesmentioning

confidence: 99%

Presence of Two Strains of <i>Neisseria gonorrhoeae</i> in Both the Cervix and Pharynx of a Patient with Disseminated Infection Caused by a Single Strain

1988

View full text Add to dashboard Cite

Neisseria gonorrhoeae was cultured from the cervix, pharynx and blood stream of an 18-year-old woman who had a disseminated gonococcal infection. Two different phenotypes of N.gonorrhoeae, distinguished on the basis of their protein 1 serovar, whole-cell protein profile, lectin-type and antibiotic susceptibility, were isolated from both the cervix and pharynx, whereas only one of these phenotypes was isolated from the blood. This finding stresses the importance of blood cultures as a prelude to determining appropriate antimicrobial therapy in cases of disseminated gonococcal infection.

show abstract

Large plasmids in an actinomycete

Cited by 13 publications

References 18 publications

A New Species of Actinomycete, Amycolata alni

A New Species of Actinomycete, Amycolata alni

Rathayibacter gen. nov., Including the Species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and Six Strains from Annual Grasses

Presence of Two Strains of <i>Neisseria gonorrhoeae</i> in Both the Cervix and Pharynx of a Patient with Disseminated Infection Caused by a Single Strain

Contact Info

Product

Resources

About