Abstract:Quantifying the passage of the large peptide protamine (Ptm) across CymA, a passive channel for cyclodextrin uptake,
is in the focus of this study. Using a reporter-pair based fluorescence membrane assay we detected the entry of Ptm into liposomes
containing CymA. The kinetics of the Ptm entry was independent of its concentration suggesting that the permeation across CymA
is the rate-limiting factor. Furthermore, we reconstituted single CymA channels into planar lipid bilayers and recorded the ion
current fluc… Show more
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