Large gradient high magnetic field affects the association of MACF1 with actin and microtubule cytoskeleton

Qian, Airong; Hu, Lifang; Gao, Xiang; Zhang, Wei; Di, Shengmeng; Tian, Zongcheng; Yang, Pengfei; Yin, Da‐Chuan; Weng, Yuanyuan; Shang, Peng

doi:10.1002/bem.20511

Cited by 53 publications

(45 citation statements)

References 44 publications

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“…cDNA microarray reveals cytoskeleton-related genes sensitive to HMGE association of MACF1 with actin and microtubule cytoskeleton [32]. Tropomodulin 3 is a ubiquitous TMOD isoform present in non-erythroid cells, where it regulates dynamic actin processes [33].…”

Section: Discussionmentioning

confidence: 99%

cDNA microarray reveals the alterations of cytoskeleton-related genes in osteoblast under high magneto-gravitational environment

Qian¹,

Di²,

Gao³

et al. 2009

ABBS

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The diamagnetic levitation as a novel ground-based model for simulating a reduced gravity environment has been widely applied in many fields. In this study, a special designed superconducting magnet, which can produce three apparent gravity levels (0, 1, and 2 g), namely high magneto-gravitational environment (HMGE), was used to simulate space gravity environment. The effects of HMGE on osteoblast gene expression profile were investigated by microarray. Genes sensitive to diamagnetic levitation environment (0 g), gravity changes, and high magnetic field changes were sorted on the basis of typical cell functions. Cytoskeleton, as an intracellular load-bearing structure, plays an important role in gravity perception. Therefore, 13 cytoskeleton-related genes were chosen according to the results of microarray analysis, and the expressions of these genes were found to be altered under HMGE by real-time PCR. Based on the PCR results, the expressions of WASF2 (WAS protein family, member 2), WIPF1 (WAS/WASL interacting protein family, member 1), paxillin, and talin 1 were further identified by western blot assay. Results indicated that WASF2 and WIPF1 were more sensitive to altered gravity levels, and talin 1 and paxillin were sensitive to both magnetic field and gravity changes. Our findings demonstrated that HMGE can affect osteoblast gene expression profile and cytoskeleton-related genes expression. The identification of mechanosensitive genes may enhance our understandings to the mechanism of bone loss induced by microgravity and may provide some potential targets for preventing and treating bone loss or osteoporosis.

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Section: Discussionmentioning

confidence: 99%

cDNA microarray reveals the alterations of cytoskeleton-related genes in osteoblast under high magneto-gravitational environment

Qian¹,

Di²,

Gao³

et al. 2009

ABBS

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“…That is, the HMGE is a compound environment of magnetic field and gravity and it provides three apparent gravity levels as mg (weightlessness, 12 T), 1g (16 T), and 2g (hypergravity, 12 T) stably and continually for diamagnetic materials. Qian et al [21][22][23] have performed some studies about bone cells using this system and prove that it can be used to simulate weightlessness. Although this system is a complex environment, the effects induced by this environment can be analyzed in detail.…”

Section: Discussionmentioning

confidence: 99%

“…Although the increase of cell apoptosis is one of the significant consequences in cell structure and function that occurs in microgravity [31,32], such a high apoptotic rate resulted from simulated weightlessness at such a short time (for 12 h) has not been reported previously. Qian et al [17][18][19][20][21] demonstrated that the proliferation of MC3T3-E1 (inhibited) and MG-63 (accelerated) osteoblasts lines were affected by HMGE and the cell shape became more flat under mg condition. However, the apoptosis was not significant.…”

Section: Discussionmentioning

confidence: 99%

“…The effects of highmagneto-gravitational environment (HMGE) on biological materials have been reported [16][17][18][19][20]. Qian et al [20][21][22] demonstrated that HMGE affected osteoblast morphology, cytoskeleton architecture, and function. In this study, a ground-based experimental platform that could produce an abnormal gravitational environment by a large gradient high magnetic field [23] was adopted and defined as HMGE.…”

Section: Introductionmentioning

confidence: 99%

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Human mesenchymal stem cells are sensitive to abnormal gravity and exhibit classic apoptotic features

Meng

et al. 2011

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The aim of the present study was to investigate the effects of abnormal gravity on human mesenchymal stem cells (hMSCs). Strong magnetic field and magnetic field gradient generate a magnetic force that can add to or subtract from the gravitational force. In this study, this is defined as a high-magneto-gravitational environment (HMGE). The HMGE provides three apparent gravity levels, i.e. hypogravity (mg), hypergravity (2g) and normal gravity with strong magnetic field (1g) conditions. After hMSCs were subject to HMGE for 12 h, the proliferation, morphology, structure and apoptosis were investigated. Results showed that the proliferation of hMSCs was inhibited under mg condition. The abnormal gravity induced morphologic characteristics of apoptosis cells, such as cell shrinkage, membrane blebbing, nuclear chromatin condensation and margination, decreased cell viability, and increased caspase-3/7 activity. The rate of apoptosis under mg condition is up to 56.95%. The F-actin stress fibers and microtubules were disrupted under abnormal gravity condition. Under mg-condition, the expression of p53 at mRNA and protein levels was up-regulated more than 9-and 6 folds, respectively. The Pifithrin-a, an specific inhibitor of p53, inhibited the apoptosis and prevented the disruption of cytoskeleton induced by abnormal gravity. These results implied that hMSCs were sensitive to abnormal gravity and exhibited classic apoptotic features, which might be associated with p53 signaling.

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“…In this way, osteoblasts resist the disrupted cytoskeleton to a certain extent. MACF1 is widely distributed across the osteoblasts and co-localized with cytoskeleton (Becker and Souza 2013), but the colocalization is remarkably reduced under DL with accumulation at perinuclear region (Qian et al 2009b), suggesting that MACF1 may function in graviperception. Connexin 43 (Cx43) forms gap junctions and hemichannels in bone cells directing cell-cell communication, which plays pivotal roles in bone development and remodeling (Plotkin and Bellido 2013).…”

Section: Mechanotransduction Of Bone and Immune Cells Under Microgravitymentioning

confidence: 97%

Mechano-biological Coupling of Cellular Responses to Microgravity

Long

Wang

Zheng

et al. 2015

Microgravity Sci. Technol.

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Cellular response to microgravity is a basic issue in space biological sciences as well as space physiology and medicine. It is crucial to elucidate the mechanobiological coupling mechanisms of various biological organisms, since, from the principle of adaptability, all species evolved on the earth must possess the structure and function that adapts their living environment. As a basic element of an organism, a cell usually undergoes mechanical and chemical remodeling to sense, transmit, transduce, and respond to the alteration of gravitational signals. In the past decades, new computational platforms and experimental methods/techniques/devices are developed to mimic the biological effects of microgravity environment from the viewpoint of biomechanical approaches. Mechanobiology of plant gravisensing in the responses of statolith movements along the gravity vector and the relevant signal transduction and molecular regulatory mechanisms are investigated at gene, transcription, and protein levels. Mechanotransduction of bone or immune cell responses and stem cell development and tissue histogenesis are elucidated under microgravity. In this review, several important issues are briefly discussed. Future issues on gravisensing and mechanotransducing mechanisms are also proposed for ground-based studies as well as space missions.

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Large gradient high magnetic field affects the association of MACF1 with actin and microtubule cytoskeleton

Cited by 53 publications

References 44 publications

cDNA microarray reveals the alterations of cytoskeleton-related genes in osteoblast under high magneto-gravitational environment

cDNA microarray reveals the alterations of cytoskeleton-related genes in osteoblast under high magneto-gravitational environment

Human mesenchymal stem cells are sensitive to abnormal gravity and exhibit classic apoptotic features

Mechano-biological Coupling of Cellular Responses to Microgravity

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