Large fragment target enrichment and sequencing of the 4.4Mb major histocompatibility complex by region-specific extraction and HiSeq 2500

Rabenstein, Hannah; Bangol, Barbara; Linke, Suzanne; Brumm, Riccardo; Poggel, Sebastian; Eck, Sebastian; Ott, Karl‐Heinz; Dapprich, Johannes; Hirv, Kaimo

doi:10.1016/j.humimm.2015.07.081

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2016

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“…2013; Pröll et al . 2011; Rabenstein et al . 2015) to sets of smaller loci containing SNPs (Kenny et al .…”

Section: Introductionmentioning

confidence: 99%

Adapterama IV: Sequence Capture of Dual-digest RADseq Libraries with Identifiable Duplicates (RADcap)

Tj²,

Catchen

et al. 2016

Preprint

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Abstract:2 4Molecular ecologists seek to genotype hundreds to thousands of loci from hundreds to thousands 2 5 of individuals at minimal cost per sample. Current methods such as restriction site associated 2 6 DNA sequencing (RADseq) and sequence capture are constrained by costs associated with 2 7 inefficient use of sequencing data and sample preparation, respectively. Here, we demonstrate 2 8RADcap, an approach that combines the major benefits of RADseq (low cost with specific start researchers to process RADseq data using traditional pipelines, and we tested the RADcap RADcap method: 1) can methodologically reduce (to <5%) and computationally remove PCR primer costs of only a few hundred dollars.

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“…2013; Pröll et al . 2011; Rabenstein et al . 2015) to sets of smaller loci containing SNPs (Kenny et al .…”

Section: Introductionmentioning

confidence: 99%

Adapterama IV: Sequence Capture of Dual-digest RADseq Libraries with Identifiable Duplicates (RADcap)

Tj²,

Catchen

et al. 2016

Preprint

View full text Add to dashboard Cite

show abstract

Large fragment target enrichment and sequencing of the 4.4Mb major histocompatibility complex by region-specific extraction and HiSeq 2500

Cited by 1 publication

References 0 publications

Adapterama IV: Sequence Capture of Dual-digest RADseq Libraries with Identifiable Duplicates (RADcap)

Adapterama IV: Sequence Capture of Dual-digest RADseq Libraries with Identifiable Duplicates (RADcap)

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