Large Animal Model for Evaluating the Efficacy of the Gene Therapy in Ischemic Heart

Korpela, H; Siimes, Satu; Ylä‐Herttuala, Seppo

doi:10.3791/62833

Cited by 3 publications

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“…These investigations have utilized various animal models and in vitro experiments to elucidate the potential of gene therapy interventions in repairing and regenerating cardiac tissue [305][306][307].…”

Section: Gene Therapymentioning

confidence: 99%

The Long and Winding Road to Cardiac Regeneration

Sacco,

Castaldo,

Di Meglio

et al. 2023

Applied Sciences

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Cardiac regeneration is a critical endeavor in the treatment of heart diseases, aimed at repairing and enhancing the structure and function of damaged myocardium. This review offers a comprehensive overview of current advancements and strategies in cardiac regeneration, with a specific focus on regenerative medicine and tissue engineering-based approaches. Stem cell-based therapies, which involve the utilization of adult stem cells and pluripotent stem cells hold immense potential for replenishing lost cardiomyocytes and facilitating cardiac tissue repair and regeneration. Tissue engineering also plays a prominent role employing synthetic or natural biomaterials, engineering cardiac patches and grafts with suitable properties, and fabricating upscale bioreactors to create functional constructs for cardiac recovery. These constructs can be transplanted into the heart to provide mechanical support and facilitate tissue healing. Additionally, the production of organoids and chips that accurately replicate the structure and function of the whole organ is an area of extensive research. Despite significant progress, several challenges persist in the field of cardiac regeneration. These include enhancing cell survival and engraftment, achieving proper vascularization, and ensuring the long-term functionality of engineered constructs. Overcoming these obstacles and offering effective therapies to restore cardiac function could improve the quality of life for individuals with heart diseases.

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Section: Gene Therapymentioning

confidence: 99%

The Long and Winding Road to Cardiac Regeneration

Sacco,

Castaldo,

Di Meglio

et al. 2023

Applied Sciences

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“…These CD163 knock out pigs were fully resistant to the virus that causes porcine respiratory and reproductive syndrome (PRRS) (Whitworth et al 2016 ). In addition to creating pigs with disease resistance traits, gene editing can be used to improve production traits (Telugu et al 2017 ), to lessen the impact on the environment (Forsberg et al 2013 ), improve digestion (Guan et al 2017 ) as well as to understand reproductive performance (Johns et al 2021 ) Pigs are also used as models for evaluating somatic cell genome editing, as well as for gene therapy (Korpela et al 2021 ; Liu et al 2021 ). Gene editing is explored across the livestock industry to improve animal welfare and increasing efficiency.…”

Section: Introductionmentioning

confidence: 99%

Improvements in pig agriculture through gene editing

et al. 2022

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Genetic modification of animals via selective breeding is the basis for modern agriculture. The current breeding paradigm however has limitations, chief among them is the requirement for the beneficial trait to exist within the population. Desirable alleles in geographically isolated breeds, or breeds selected for a different conformation and commercial application, and more importantly animals from different genera or species cannot be introgressed into the population via selective breeding. Additionally, linkage disequilibrium results in low heritability and necessitates breeding over successive generations to fix a beneficial trait within a population. Given the need to sustainably improve animal production to feed an anticipated 9 billion global population by 2030 against a backdrop of infectious diseases and a looming threat from climate change, there is a pressing need for responsive, precise, and agile breeding strategies. The availability of genome editing tools that allow for the introduction of precise genetic modification at a single nucleotide resolution, while also facilitating large transgene integration in the target population, offers a solution. Concordant with the developments in genomic sequencing approaches, progress among germline editing efforts is expected to reach feverish pace. The current manuscript reviews past and current developments in germline engineering in pigs, and the many advantages they confer for advancing animal agriculture.

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The Long and Winding Road to Cardiac Regeneration

Sacco¹,

Castaldo²,

Meglio³

et al. 2023

Preprint

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Cardiac regeneration is a critical endeavor in the treatment of heart diseases, aimed at repairing and enhancing the structure and function of damaged myocardium. This review offers a comprehensive overview of current advancements and strategies in cardiac regeneration, with a specific focus on regenerative medicine and tissue engineering-based approaches. Stem cell-based therapies, which involve the utilization of adult stem cells and pluripotent stem cells hold immense potential for replenishing lost cardiomyocytes and facilitating cardiac tissue repair and regeneration. Tissue engineering also plays a prominent role employing synthetic or natural biomaterials, engineering cardiac patches and grafts with suitable properties, and fabricating upscale bioreactors to create functional constructs for cardiac recovery. These constructs can be transplanted into heart to provide mechanical support and facilitate tissue healing. Additionally, the production of organoids and chips that accurately replicate the structure and function of the whole organ is an area of extensive research. Despite significant progress, several challenges persist in the field of cardiac regeneration. These include enhancing cell survival and engraftment, achieving proper vascularization, and ensuring the long-term functionality of engineered constructs. Overcoming these obstacles offering effective therapies to restore cardiac function could improve the quality of life for individuals with heart diseases.

show abstract

Large Animal Model for Evaluating the Efficacy of the Gene Therapy in Ischemic Heart

Cited by 3 publications

References 0 publications

The Long and Winding Road to Cardiac Regeneration

The Long and Winding Road to Cardiac Regeneration

Improvements in pig agriculture through gene editing

The Long and Winding Road to Cardiac Regeneration

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