Laparoscopical intrauterine insemination with different doses of fresh, conserved, and frozen semen for the production of ovine zygotes

Ehling, Christine; Wirth, Petra; Schindler, Lothar; Hadeler, Klaus‐Gerd; Döpke, Hans-Hermann; Lemme, Erika; Herrmann, Doris; Niemann, Heinrich

doi:10.1016/s0093-691x(03)00049-9

Cited by 25 publications

(17 citation statements)

References 36 publications

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“…The use of freshly diluted semen could give the best result: 70 to 82 % (Donovan et al, 2001 and and 82.2% (Hill et al, 1998;Ehling et al, 2003). A similar level could be reached with cooled and stored semen /74-76% (Gergátz&Gyökér, 1997) and 56.7% (Fernandez-Abella et al, 2003) as well as with chilled and stored semen /between 37.5% (Fernandez-Abella et al, 2003) and 64.2-73.33% (Stefanov et al, 2006)/.…”

Section: The Use Of Fresh Cooled Chilled Frozen Semenmentioning

confidence: 92%

“…About 50 to 100 million spermatozoa is needed in one dose to vaginal insemination, but 25 to 50 million are suggested for cervical insemination and 15 to 20 million is enough for laparoscopic AI (Kukovics, 1974;Jávor et al, 2006;Gergátz, 2007). However, several scientists have used much higher numbers: 106 (Fernandez-Abella et al, 2003), 50 to 300 (Ehling et al, 2003) and 400 (King et al, 2004) million spermatozoa in one dose of semen.…”

Section: Dose Of Inseminated Semenmentioning

confidence: 99%

See 1 more Smart Citation

Artificial Insemination of Sheep - Possibilities, Realities and Techniques at the Farm Level

Kukovics¹,

Gyoker²,

Németh³

et al. 2011

Artificial Insemination in Farm Animals

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Section: The Use Of Fresh Cooled Chilled Frozen Semenmentioning

confidence: 92%

Section: Dose Of Inseminated Semenmentioning

confidence: 99%

Artificial Insemination of Sheep - Possibilities, Realities and Techniques at the Farm Level

Kukovics¹,

Gyoker²,

Németh³

et al. 2011

Artificial Insemination in Farm Animals

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“…roscopic AI with fresh semen enables sperm number to be reduced to <50 million sperm per dose (Ehling et al, 2003), which limits the number of doses per ejaculate to <100 ewes to achieve pregnancy rates >70% (Sayre and Lewis, 1997). This coupled with the seasonality of sheep production requires a large number of rams to enter AI to facilitate a genetic improvement program.…”

Section: Introductionmentioning

confidence: 99%

Effect of exposing rams to a female stimulus before semen collection on ram libido and semen quality1

Fahey

Duffy

Fair

2012

Journal of Animal Science

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“…It also provides a controlled semen placement, but irritations of the endometrium by manipulating the endoscope in the uterus or by the use of a pneumouterus in a non-surgical deep intrauterine insemination procedure are minimised. Laparoscopic intrauterine insemination is successfully used in sheep for different purposes (Gourley and Riese 1990;Moses et al 1997;Ehling et al 2003;PereyraBonnet et al 2011). In pigs such a method has been used only to a limited extent so far (Fantinati et al 2005;Brüssow et al 2006Brüssow et al , 2011.…”

Section: Discussionmentioning

confidence: 99%

Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos

et al. 2013

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Double fixed-time insemination after ovulation induction is commonly used in pigs to obtain in vivo produced embryos at defined stages of development for downstream biotechnological applications. However, variations in the time of ovulation and fertilisation of the ovulated oocytes by spermatozoa, mainly in one of the inseminations, can cause diversities in embryo development. The aim of the present study was to reduce embryo diversity and to achieve a 'uniform outcome' of porcine embryo stages using single laparoscopic fixedtime insemination (LIUI). Altogether, 48 puberal German Landrace gilts were included in the study. Estrus of gilts was synchronized by 15-day long altrenogest (Regumate ® ) feeding and follicle development was stimulated with 850 IU eCG 24 h after the final altrenogest application. Ovulation was induced with 500 IU hCG 80 h after eCG. LIUI was performed 31 h after hCG treatment. Gilts under general anaesthesia were fixed in a dorsal position, a pneumoperitoneum was produced and three trocar cannulas were inserted into the abdomen for optics and instruments. Each uterine horn was carefully punctured 10-15 cm caudal from the utero-tubal junction with a 2.5 mm trocar. A 2.2 mm catheter was inserted about 3 cm into the uterine lumen and 20 ml of extended fresh boar semen (32.2 × 106 sperm cells/ml) were injected. Embryos were surgically flushed from the genital tract two (Day 2) and three (Day 3) days after insemination. Altogether, 778 oocytes/embryos were recovered (recovery rate 68 ± 17%); 45 of 48 gilts (93.8%) revealed fertilisation and 76.1% of the recovered embryos (n = 592) were at the 2-and 4-cell stage. On Day 2 (n = 22 gilts), a higher percentage of gilts (72.7%, P < 0.05) displayed only 2-cell embryos compared with gilts which had 2-and 4-cell (22.7%), or only 4-cell embryos (4.6%). On Day 3 (n = 23 gilts), the proportion of gilts with 2-cell, 2-and 4-cell, and only 4-cell embryos shifted to 4.3%, 0% and 95.7%, respectively (P < 0.05). The results of the present study demonstrate high rates of fertilisation and homogenously developed embryos after single fixed-time laparoscopic intrauterine insemination in gilts. Additionally, these results were achieved by inseminating a 60% lower number of sperm cells per insemination dose compared to usual doses used for transcervical insemination. In conclusion, LIUI can be recommended for the in vivo production of embryos in a homogeneous developmental stage, and also as an alternative method for low-dose insemination. Keywords: laparoscopic insemination; embryo diversity; pigList of abbreviations BW = body weight, CL = corpus luteum, eCG = equine chorionic gonadotropin, hCG = human chorionic gonadotropin, IU = international unit, LIUI = laparoscopic fixed-time inseminationIn vivo-derived embryos at a defined stage of development are often a necessary requirement for biotechnological applications and research. However, different developmental stages of the embryos (embryo diversity) at the time point of recovery remain an issue pigs (S...

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Laparoscopical intrauterine insemination with different doses of fresh, conserved, and frozen semen for the production of ovine zygotes

Cited by 25 publications

References 36 publications

Artificial Insemination of Sheep - Possibilities, Realities and Techniques at the Farm Level

Artificial Insemination of Sheep - Possibilities, Realities and Techniques at the Farm Level

Effect of exposing rams to a female stimulus before semen collection on ram libido and semen quality1

Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos

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