Laparoscopic Diagnosis and Treatment of Aortic Vascular Prosthetic Graft Infections in a Porcine Model

Gao, H.; Lund, Lars; Prag, Jørgen; Sandermann, Jes; Lindholt, Jes Sanddal

doi:10.1016/j.ejvs.2007.07.005

Cited by 13 publications

(9 citation statements)

References 24 publications

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“…For all organ systems, except for sepsis, eye, urinary and genital, porcine models with a time frame of more than four weeks have been developed. 43,48–51 However, in 69% of all the models included in this review the time-frame was below two weeks (Figure 3(e)).…”

Section: Resultsmentioning

confidence: 99%

Guidelines for porcine models of human bacterial infections

2018

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During the last 10 years the number of porcine models for human bacterial infectious diseases has increased. In the future, this tendency is expected to continue and, therefore, the aim of the present review is to describe guidelines for the development and reporting of these models. The guidelines are based on a review of 122 publications of porcine models for different bacterial infectious diseases in humans. The review demonstrates a substantial lack of information in most papers which hampers reproducibility and continuation of the work that was established in the models. The guidelines describe overall principles related to the inoculum, the animal, the infected animal and the post-mortem characterization that are of crucial importance when porcine models of infectious diseases are developed, validated and reported.

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Section: Resultsmentioning

confidence: 99%

Guidelines for porcine models of human bacterial infections

2018

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“…Various antibiotics have been evaluated in vitro in the setting of VGI [ 31 , 32 , 10 , 33 – 35 ]. However, the effects of gentamicin-coated vascular grafts are not well defined yet [ 18 , 19 , 13 ].…”

Section: Discussionmentioning

confidence: 99%

Treatment of vascular graft infections: gentamicin-coated ePTFE grafts reveals strong antibacterial properties in vitro

Lazic

Obermeier

Dietmair

et al. 2022

J Mater Sci: Mater Med

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Vascular graft infections (VGI) are severe complications in prosthetic vascular surgery with an incidence ranging from 1 to 6%. In these cases, synthetic grafts are commonly used in combination with antimicrobial agents. Expanded polytetrafluoroethylene (ePTFE) is in clinical use as a synthetic graft material and shows promising results by influencing bacterial adhesion. However, the literature on antibiotic-bound ePTFE grafts is scarce. Gentamicin is a frequently used antibiotic for local treatment of surgical site infections, but has not been evaluated as antimicrobial agent on ePTFE grafts. In this study, we examine the antimicrobial efficacy and biocompatibility of novel types of gentamicin-coated ePTFE grafts in vitro. ePTFE grafts coated with gentamicin salt formulations with covalently-bound palmitate were evaluated in two drug concentrations (GP1.75% and GP3.5%). To investigate effects from types of formulations, also suspensions of gentamicin in palmitate as well as polylactide were used at comparable levels (GS + PA and GS + R203). Antibacterial efficacies were estimated by employing a zone of inhibition, growth inhibition and bacterial adhesion assay against Staphylococcus aureus (SA). Cytotoxicity was determined with murine fibroblasts according to the ISO standard 10993-5. Gentamicin-coated ePTFE grafts show low bacterial adherence and strong antibacterial properties in vitro against SA. Bactericidal inhibition lasted until day 11. Highest biocompatibility was achieved using gentamicin palmitate GP1.75% coated ePTFE grafts. ePTFE grafts with gentamicin-coating are effective in vitro against SA growth and adherence. Most promising results regarding antimicrobial properties and biocompatibility were shown with chemically bounded gentamicin palmitate GP1.75% coatings.

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“…We used Sinclair miniature pigs in this study because this strain is free of many common infectious diseases and is often used in cardiovascular research, especially in studies of aortic disease. 19 Although dog models have been used to study perioperative graft infections, 20,21 using large animals is costly and time-consuming because general anesthesia with tracheal intubation and large experimental facilities are required. 22 To minimize these expenses, we implanted the grafts in the abdominal aorta rather than in the thoracic aorta and used roof-patch angioplasty rather than a tube- graft replacement to simplify the technique and reduce operative and postoperative morbidities.…”

Section: Discussionmentioning

confidence: 99%

A pilot study of a triple antimicrobial-bonded Dacron graft for the prevention of aortic graft infection

Aboshady

Raad

Shah

et al. 2012

Journal of Vascular Surgery

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Laparoscopic Diagnosis and Treatment of Aortic Vascular Prosthetic Graft Infections in a Porcine Model

Abstract: Laparoscopy is a potential diagnostic tool for aortic graft infection and also affords the opportunity to carry out bacteriological sampling and local antibiotic treatment. The efficacy of laparoscopic treatment needs further evaluation.

Cited by 13 publications

References 24 publications

Guidelines for porcine models of human bacterial infections

Guidelines for porcine models of human bacterial infections

Treatment of vascular graft infections: gentamicin-coated ePTFE grafts reveals strong antibacterial properties in vitro

A pilot study of a triple antimicrobial-bonded Dacron graft for the prevention of aortic graft infection

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