Lanthanide Nanoprobes for the Multiplex Evaluation of Breast Cancer Biomarkers

Chen, Xue; Song, Hongjie; Li, Ziyan; Liu, Rui; Lv, Yi

doi:10.1021/acs.analchem.1c03445

Cited by 12 publications

(8 citation statements)

References 46 publications

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“…[28][29][30][31] With applying the strategy of element labeling, [32][33] the sensitivity of ICP-MS 34 is well integrated into our biosensor. 35 Our work confirms that this biosensing method can meet the requirements for trace concentration TOB detection, and has the potential for the detection of a variety of antibiotics and other targets with corresponding aptamers.…”

Section: Introductionsupporting

confidence: 76%

Elemental Probe-Based CRISPR/Cas12a Biosensing For Sensitive Tobramycin Detection

Liu¹

2022

At.Spectrosc.

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The precise targeting and excellent specific cleave capability of CRISPR/Cas (clustered regularly inters-paced short palindromic repeats-associated proteins) have gained much research interest. In addition to nucleic acid detection, researchers exploring the potential of this system for detecting various other targets. Herein, we proposed a novel CRISPR/Cas biosensing based Tobramycin (TOB) detection method. Combining with the aptamer recognition and element labeling strategy, highly sensitive and specific detection of TOB is realized. Due to the powerful elemental analysis capabilities of inductively coupled plasma mass spectrometry (ICP-MS) and the excellent signal amplification effect of CRISPR/Cas, the linear concentration range for TOB was 2.8-100 nM in this method, whereas the detection limit was 0.83 nM. The proposed method has been successfully applied in the analysis of actual water samples.

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Section: Introductionsupporting

confidence: 76%

Elemental Probe-Based CRISPR/Cas12a Biosensing For Sensitive Tobramycin Detection

Liu¹

2022

At.Spectrosc.

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“…However, reaction chemistry for the labelling of proteins, via their primary amino groups or cysteine residues, is still problematic. Metal nanoparticles (NPs), such as AuNPs [ 51 , 52 ] were investigated as labels of biomolecules for detection by ICP-MS, and investigations were further extended to NPs of elements, with particular sensitivity in ICP-MS detection, i.e., NaEuF, NaTbF, and NaHoF [ 53 ]. Notably, a considerable part of such chelates and polymers containing NP-types does not contribute to the amplification of the analytical signal.…”

Section: Elemental Tagging In Inorganic Mass Spectrometric Bioanalysismentioning

confidence: 99%

Review about Powerful Combinations of Advanced and Hyphenated Sample Introduction Techniques with Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) for Elucidating Trace Element Species in Pathologic Conditions on a Molecular Level

Michalke

2022

IJMS

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Element analysis in clinical or biological samples is important due to the essential role in clinical diagnostics, drug development, and drug-effect monitoring. Particularly, the specific forms of element binding, actual redox state, or their spatial distribution in tissue or in single cells are of interest in medical research. This review summarized exciting combinations of sophisticated sample delivery systems hyphenated to inductively coupled plasma-mass spectrometry (ICP-MS), enabling a broadening of information beyond the well-established outstanding detection capability. Deeper insights into pathological disease processes or intracellular distribution of active substances were provided, enabling a better understanding of biological processes and their dynamics. Examples were presented from spatial elemental mapping in tissue, cells, or spheroids, also considering elemental tagging. The use of natural or artificial tags for drug monitoring was shown. In the context of oxidative stress and ferroptosis iron, redox speciation gained importance. Quantification methods for Fe2+, Fe3+, and ferritin-bound iron were introduced. In Wilson’s disease, free and exchangeable copper play decisive roles; the respective paragraph provided information about hyphenated Cu speciation techniques, which provide their fast and reliable quantification. Finally, single cell ICP-MS provides highly valuable information on cell-to-cell variance, insights into uptake of metal-containing drugs, and their accumulation and release on the single-cell level.

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“…Attributed to the excellent resolution of the mass spectrometer, elemental isotopes with different mass-to-charge ratios can be clearly distinguished by ICP–MS. So far, the ICP–MS-based multiplex assay has been successfully applied in the quantification of a variety of biomarkers with various elemental tags. − Among the different elemental tags, lanthanide tags are most widely used due to their advantages of diversity, ease of preparation, low biological background, and high sensitivity in ICP–MS. − de Bang et al first used lanthanide-labeled DNA probes to recognize miRNAs on northern blot membranes, and the labeled miRNAs were directly detected by laser ablation (LA)-ICP–MS.…”

Section: Introductionmentioning

confidence: 99%

Simple Amplifier Coupled with a Lanthanide Labeling Strategy for Multiplexed and Specific Quantification of MicroRNAs

Kang

Chen

et al. 2022

Anal. Chem.

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Inductively coupled plasma–mass spectrometry (ICP–MS) with elemental labeling is a promising strategy for multiplex microRNA (miRNA) analysis. However, it is still challenging for specific analysis of multiple miRNAs with high homology, and the development of multiplex assays is always limited by the complexity of the sequence design. Herein, a simple and direct ICP–MS-based assay was developed for the simultaneous detection of three miRNAs by combining the lanthanide labeling strategy with entropy-driven catalytic (EDC) amplification. Owing to the specificity of EDC for nucleic acid recognition, it is able to differentiate miRNAs with single-base mutation in each EDC circuit. A universal biotin-labeled DNA strand was designed to hybridize with the DNA substrates for three EDC circuits, targeting miRNA-21, miRNA-155, and miRNA-10b, respectively. All the substrates were loaded on the surface of streptavidin magnetic beads. In the presence of target miRNA, the EDC reaction was initiated, and EDC substrates were dissociated, continuously releasing reporter strands that were labeled with lanthanides (Tb/Ho/Lu). After magnetic separation, the supernatant containing the released reporter strands was introduced into an ICP–MS system for simultaneous detection of 159Tb/165Ho/175Lu and quantification of miRNA-21, miRNA-155, and miRNA-10b, respectively. The limits of detection were 7.4, 7.5, and 11 pmol L–1 for miRNA-21, miRNA-155, and miRNA-10b, respectively. Overall, this study provides a powerful strategy for simultaneous quantification of multiple miRNAs, with the advantages of flexible probe design, good sensitivity, and excellent specificity.

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Lanthanide Nanoprobes for the Multiplex Evaluation of Breast Cancer Biomarkers

Cited by 12 publications

References 46 publications

Elemental Probe-Based CRISPR/Cas12a Biosensing For Sensitive Tobramycin Detection

Elemental Probe-Based CRISPR/Cas12a Biosensing For Sensitive Tobramycin Detection

Review about Powerful Combinations of Advanced and Hyphenated Sample Introduction Techniques with Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) for Elucidating Trace Element Species in Pathologic Conditions on a Molecular Level

Simple Amplifier Coupled with a Lanthanide Labeling Strategy for Multiplexed and Specific Quantification of MicroRNAs

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