Lanthanide-mediated DNA hydrolysis

Franklin, Sonya J.

doi:10.1016/s1367-5931(00)00191-5

Cited by 276 publications

(80 citation statements)

References 47 publications

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“…As Lewis acid, some lanthanide ions and their complexes have been shown to be particularly reactive for hydrolyzing phosphate esters. 66,67 Cerium, the only lanthanide that is easily oxidized to the tetravalent state, has received great attention. 67 Recent studies have shown that CeONP can hydrolyze phosphate ester bonds of many biologically relevant molecules.…”

Section: Nitric Oxide Radical Scavengingmentioning

confidence: 99%

“…66,67 Cerium, the only lanthanide that is easily oxidized to the tetravalent state, has received great attention. 67 Recent studies have shown that CeONP can hydrolyze phosphate ester bonds of many biologically relevant molecules. 46,68,69 Qian and colleagues 68 found that CeONP could efficiently mediate the dephosphorylation of phosphopeptides.…”

Section: Nitric Oxide Radical Scavengingmentioning

confidence: 99%

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Cerium oxide nanoparticle: a remarkably versatile rare earth nanomaterial for biological applications

2014

NPG Asia Mater

891

650

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Section: Nitric Oxide Radical Scavengingmentioning

confidence: 99%

Section: Nitric Oxide Radical Scavengingmentioning

confidence: 99%

Cerium oxide nanoparticle: a remarkably versatile rare earth nanomaterial for biological applications

2014

NPG Asia Mater

891

650

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“…As shown in lane 2 of Figure 10, site-selective scission occurred at the 5-base gap site, thus producing the corresponding five bands. The DNA scission efficiency increased with an increasing concentration of Ce(NO 3 ) 3 and attained a plateau when the ratio of Ce(NO 3 ) 3 to EDTP was approximately 2-4. These results support that a well-defined Ce(IV) complex on the EDTP ligand is successfully prepared from the Ce(III) precursor, although its stoichiometry has not yet been clearly determined.…”

Section: Preparation Of An Artificial Dna Cutter From Ce(iii) As a Prmentioning

confidence: 98%

“…On this basis, chemists, biochemists and biologists have long attempted to rewrite the information encoded within DNA using various approaches. One direct and straightforward approach to precisely alter the information contained by DNA is the site-selective scission of DNA at a target site, [1][2][3][4][5][6][7][8][9][10][11] followed by the appropriate manipulation of the DNA at this local site.…”

Section: Introductionmentioning

confidence: 99%

Artificial site-selective DNA cutters to manipulate single-stranded DNA

Aiba

Komiyama

2012

Polym J

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Recent progress regarding the development of artificial site-selective DNA cutters by chemical approaches are reviewed herein, with a special focus on site-selective cutters for single-stranded DNA. We employ a Ce(IV)/EDTA complex to serve as the catalyst, because it efficiently and selectively cuts single-stranded DNA. Using two complementary oligonucleotide additives, a gap structure is formed at the target site in the single-stranded DNA substrate. Owing to the substrate specificity of Ce(IV)/ EDTA, the gap site is preferentially hydrolyzed, resulting in a site-selective DNA scission. The scission site is easily determined using the Watson-Crick base-pairing rule; thus, both the sequence and scission specificity can be tuned according to demand. The site-selective scission is greatly promoted by attaching a multiphosphonate to the termini of the oligonucleotide additives and placing this ligand at the gap site. The scission fragments can be connected with foreign DNA using ligase, and the recombinant DNA expresses the corresponding protein in E. coli. INTRODUCTIONThe role of DNA in storing and carrying genetic information is essential for the function and development of all living organisms. If the information stored within DNA can be rewritten by scientists, numerous applications in a variety of fields will be possible. On this basis, chemists, biochemists and biologists have long attempted to rewrite the information encoded within DNA using various approaches. One direct and straightforward approach to precisely alter the information contained by DNA is the site-selective scission of DNA at a target site, 1-11 followed by the appropriate manipulation of the DNA at this local site.Currently, in molecular biology, DNA is manipulated by the 'cut-and-paste' method. In this approach, DNA is first cut at a predetermined site by naturally occurring restriction enzymes, and the resultant fragment is connected with another DNA fragment using ligases. Here, the target gene is inserted, deleted or altered. This strategy is satisfactorily effective so long as small DNAs such as plasmids (composed of several thousand base pairs) are targeted. Unfortunately, this technology is insufficient for manipulating large DNAs; however, our interests have been gradually focusing on the manipulation of genomic DNAs of higher organisms. The key problem is the low site specificity of naturally occurring restriction enzymes (most of them recognize 4 to 8-bp DNA sequences). Statistically, the scission site of a restriction enzyme with 6-bp

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“…One large group of artificial nucleases are the synthetic catalytic agents, mostly metal complexes, with all their advantages and drawbacks discussed in detail elsewhere [5,[22][23][24][25]. The other class of the designed enzymes includes modified versions of native proteins constructed either by introducing mutations through molecular design or directed evolution methods or by creating chimera from independent catalytic and DNA binding domains.…”

Section: Current Artificial Nucleasesmentioning

confidence: 99%

Rescue of the activity of HNH nuclease mutants - towards controlled enzymes for gene therapy

Gyurcsik

2016

CPPS

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Artificial nucleases are designed for in vivo gene engineering, as the DNA cleavage performed at a specific target site enhances the effectiveness of the cell's DNA repair machinery. The therapeutic potential of the above phenomenon stems from the knowledge that (i) the shifted reading frame can be restored by non-homologous end-joining, or (ii) a DNA of erroneous sequence -causing a genetic disease -can be corrected by homologous recombination in the presence of a suitable DNA template. Besides the advantageous properties of the nowadays applied zinc finger nucleases, TALE nucleases and the CRISPR/Cas9 system, they possess a residual citotoxicity. This is related to offtarget cleavages, which could be prevented by the strict regulation of the enzymes. The studies on enzymes acting naturally in a controlled manner are beneficial to get better insight into their mechanism. Such enzymes or their appropriate domains may be the most promising alternatives to the presently applied ones. As an example, the DNA cleavage of the inactive HNH nuclease mutants is inducible in a multiple way. This property may be used for establishing a control mechanism and thus, in combination with specific DNA-binding domains they are good candidates for the catalytic site of artificial nucleases. Here we collect the results on the properties of the HNH nucleases that allow for their redesign into enzymes with possible therapeutic applications.

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Lanthanide-mediated DNA hydrolysis

Cited by 276 publications

References 47 publications

Cerium oxide nanoparticle: a remarkably versatile rare earth nanomaterial for biological applications

Cerium oxide nanoparticle: a remarkably versatile rare earth nanomaterial for biological applications

Artificial site-selective DNA cutters to manipulate single-stranded DNA

Rescue of the activity of HNH nuclease mutants - towards controlled enzymes for gene therapy

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