Lanthanide Complex for Single-Molecule Fluorescent in Situ Hybridization and Background-Free Imaging
Fei Su,
Shiyu Chen,
Yuanhua Liu
et al.
Abstract:Traditional single-molecule fluorescence in situ hybridization
(smFISH) methods for RNA detection often face sensitivity challenges
due to the low fluorescence intensity of the probe. Also, short-lived
autofluorescence complicates obtaining clear signals from tissue sections.
In response, we have developed an smFISH probe using highly grafted
lanthanide complexes to address both concentration quenching and autofluorescence
background. Our approach involves an oligo PCR incorporating azide-dUTP,
enabling conjug… Show more
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