Lantadene A-induced apoptosis in human leukemia HL-60 cells

Sharma, Monika; Sharma, Praveen; Bansal, M. P.; Singh, Jaswant

doi:10.4103/0253-7613.33433

Cited by 31 publications

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“…Apoptosis is a physiological process characterized by chromatin condensation, membrane blebbing, cell shrinkage and DNA fragmentation (Sharma et al 2007). Cytotoxic assay resulted several cytogenetic aberrations having features indicative of cell death.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and apoptotic activities of extract of Amaranthus spinosus L. in Allium cepa and human erythrocytes

Prajitha

Thoppil

2016

Cytotechnology

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The present study examined the apoptosis inducing effects of Amaranthus spinosus L. aqueous extract in Allium cepa root meristematic cells and human erythrocytes. Cytogenetic assay revealed many apoptosis inducing cytogenetic aberrations viz., cytoplasmic breakage, cytoplasmic disintegration, cytoplasmic shrinkage, receding of cytoplasm, cytoplasmic vacuolation, enucleated cell, ghost cell, nuclear vacuolation, nuclear fragmentation and nuclear disintegration. A remarkable modification of red blood cell surface morphology was observed in the result of RBC assay. The treated RBCs show membrane blebbing and shrinkage, features typical for apoptosis in nucleated cells. Significant induction of cell death was observed in treated Allium root tip cells after Evans blue staining, disclosing the membrane damage potential of the plant extract. TTC assay results in reduced mitochondrial/ metabolic activity in Allium root tip cells after treatment, designating the adverse effect of plant extract on mitochondrial respiratory chain. These results confirm the apoptosis inducing potential of A. spinosus extract. Confirming the present results by further in vitro studies, it can be effectively targeted against cell proliferation during cancer treatment by inducing apoptosis. Thus from the present investigation it can be concluded that the aqueous extract of A. spinosus exhibited apoptosis induction and cytotoxic activities.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic and apoptotic activities of extract of Amaranthus spinosus L. in Allium cepa and human erythrocytes

Prajitha

Thoppil

2016

Cytotechnology

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“…Mahato et al 76 isolated a novel flavonol glycoside, named camaraside (12), an anti-tumor agent from L. camera 76 . In addition, Sharma et al 77 showed that lantadene A (13), a torpedoed component of L. camera, induced concentration and time-dependent inhibition of HL-60 cell proliferation by cell cycle arrest in the G0/G1 phase. Lantadene A also induced efficient HL-60 cell apoptosis by activating the caspase-3 pathway, and via the down-and up-regulation of Bcl-2 and Bax expression respectively 77 .…”

Section: Lantana Camara Lmentioning

confidence: 99%

Anti-cancer potential of South Asian plants

Rahman

2013

Nat. Prod. Bioprospect.

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Phyto-chemicals are increasingly being used in the treatment of cancer because of their availability, potential anti-cancer activity with less adverse effects when compared with chemotherapy. The variation of climate and geography in South Asian countries provides a nursing environment for the growth of versatile plant species, that are repeatedly drawing attention of the scientific community. In this review, we have focused on the anti-cancer potential of thirty plants, which are commonly found in Bangladesh, India, Nepal, Pakistan and Sri Lanka, with their mechanisms of action. In particular, we have discussed the bio-active components that display anti-cancer activity, which have been identified in these plants. This review may help researchers to profile plants with known anti-cancer effect of this region and further investigations of anti-cancer agents in medicinal plants from South Asia.

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“…8 Thus, modulating or inducing apoptosis might be helpful in cancer therapy or preventions. 7,9 Advanced nanomaterialbased drugs may offer the possibility of new and intriguing opportunities to eliminate the toxicity in human cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells

Hossain¹,

Kleve²

2011

IJN

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Background The ability to evade apoptosis is one of the key properties of cancer. The apoptogenic effect of nickel nanowires (Ni NWs) on cancer cell lines has never been adequately addressed. Due to the unique physicochemical characteristics of Ni NWs, we envision the development of a novel anticancer therapeutics specifically for pancreatic cancer. Thus, we investigated whether Ni NWs induce ROS-mediated apoptosis in human pancreatic adenocarcinoma (Panc-1) cells. Methods In this study Ni NWs were fabricated using the electrodeposition method. Synthesized Ni NWs were physically characterized by energy dispersive X-ray analysis, UV-Vis spectroscopy of NanoDrop 2000 (UV-Vis), magnetization study, scanning electron microscopy, and transmission electron microscopy. Assessment of morphological apoptotic characteristics by phase contrast microscopy (PCM), Ni-NWs-induced apoptosis staining with ethidium bromide (EB) and acridine orange (AO) followed by fluorescence microscopy (FM) was performed. For molecular biological and biochemical characterization, Panc-1 cell culture and cytotoxic effect of Ni NWs were determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Quantitative apoptosis was analyzed by flow cytometry staining with propidium iodide through cell cycle arrest and generation of ROS using 2′, 7′-dichlorofluorescein diacetate fluorescence intensity. In all experiments, Panc-1 cancer cells without any treatment were used as the negative controls. Results The intracellular uptake of Ni NWs through endocytosis by Panc-1 cells was observed by PCM. EB and AO staining of FM and MTT assay qualitatively and quantitatively confirmed the extent of apoptosis. Flow cytometric cell cycle arrest and ROS generation indicated Ni NWs as inducers of apoptotic cell death. Conclusion We investigated the role of Ni NWs as inducers of ROS-mediated apoptosis in Panc-1 cells. These results suggested that Ni NWs could be an effective apoptotic agent for Panc-1 cells and have good potential for further research into a clinical treatment selective for pancreatic cancer.

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Lantadene A-induced apoptosis in human leukemia HL-60 cells

Abstract: of apoptosis is an efficient method of treating cancer. [7] Triterpenoids represent a diverse class of natural products. Recently, pentacyclic triterpenoids have been described to induce apoptosis in different cell types. [8] Lantana camara L.

Cited by 31 publications

References 0 publications

Cytotoxic and apoptotic activities of extract of Amaranthus spinosus L. in Allium cepa and human erythrocytes

Cytotoxic and apoptotic activities of extract of Amaranthus spinosus L. in Allium cepa and human erythrocytes

Anti-cancer potential of South Asian plants

Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells

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