“…Langmuir−Blodgett (L-B) thin-film technique has been a key manufacturing tool in nanotechnology, with applications ranging from biotechnology (membranes and biosensors), gas sensors, and electronic devices to lithography. − Interest in L-B technique is fueled by its merits, which include (a) precise control of the monolayer thickness, (b) homogeneous deposition of the monolayers over large areas, and (c) the possibility of generating multilayer structures with varying layer composition on almost any kind of solid substrate. , It has been observed that L-B monolayers of metal complexes have properties similar to those of supramolecular structures involved in the biosensing of Li + , Na + , K + , Ca 2+ , etc. , Therefore, incorporation of L-B molecular layers of chelating groups onto solid platforms is expected to reveal meaningful applications in sensing techniques and other fundamental studies. However, such implications of L-B film monolayers in toxicity validation have remained only vague conjecture, especially in the emerging areas of naked-eye sensing.…”