Landscape of genes in hospital wastewater breaking through the defense line of last-resort antibiotics

Zhu, Lin; Shuai, Xin-Yi; Lin, Ze-Jun; Sun, Yujie; Zhou, Zhen-Chao; Meng, Ling-Xuan; Zhu, Yong‐Guan; Chen, Hong

doi:10.1016/j.watres.2021.117907

Cited by 20 publications

(16 citation statements)

References 48 publications

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“…The mcr -1– mcr -10 genes have been identified in isolated strains from and/or directly in environmental samples ( Anyanwu et al., 2020 ). Our previous study also reported the prevalence of mcr in aquatic environments, including watersheds and wastewaters ( Zhu et al., 2020 ; Zhu et al., 2021 ), and the current study demonstrated that mcr is a core resistance gene in soil environments, indicating that contamination by colistin resistance genes is widespread in nature and anthropogenic environments. Here mcr -1 and mcr -5 were detected, and the absence of mcr -2, mcr -3, and mcr -4 in manure-amended soils was demonstrated.…”

Section: Discussionsupporting

confidence: 73%

“…The resistome of soil samples revealed that ARGs against antibiotics which are widely used in veterinary medicine as well as clinically critical ARGs (CCARGs) persisted in manure-amended soils. Beta-lactam antibiotics (third-generation cephalosporins and carbapenems), polymyxin, and tigecycline are essential for the treatment of human Gram-negative bacterial infections ( Zhu et al., 2021 ), and vancomycin is considered to be a “last-resort” therapy for severe infections that are caused by Gram-positive bacteria ( Mühlberg et al., 2020 ). The excessive use of “last-resort” antibiotics will result in the emergence of “superbugs”, and ARGs against “last-resort” antibiotics are particularly crucial.…”

Section: Introductionmentioning

confidence: 99%

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The profile and persistence of clinically critical antibiotic resistance genes and human pathogenic bacteria in manure-amended farmland soils

Zhu

Lian

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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IntroductionMicrobial contamination in farmlands is usually underestimated and understudied. Different fertilization times and manure origins might introduce and change the microorganism diversity in farmland soils and thus might influence the abundance and persistence of microbial contamination including antibiotic resistance genes (ARGs), human bacterial pathogens (HBPs), and virulence factor genes (VFGs).MethodsA 0.5-/1.5-year fertilization experiment was performed, and metagenomic sequencing was conducted to quantify microbial contamination. The resistomes of soil samples revealed that ARGs against antibiotics which were extensively used in veterinary medicine as well as clinically critical ARGs (CCARGs) persisted in manure-amended soils. Here the extended-spectrum beta-lactamase and carbapenemase bla genes, the high-level mobilized colistin resistance gene mcr, the tigecycline resistance gene tet(X), and the vancomycin resistance gene van, all of which can circumvent the defense line of these “last-resort” antibiotics were selected to investigate CCARG pollution in farm environments.ResultsA total of 254 potential HBPs and 2106 VFGs were detected in soil samples. Overall, our results revealed that (1) farmland soils could serve as a reservoir of some important bla, mcr, tet(X), and van gene variants, (2) the diversity and relative abundance of HBPs and VFGs increased significantly with incremental fertilization times and were discrepant among different manureamended soils, and (3) most CCARGs and VFGs coexisted in HBPs.DisscusionThe results of this study suggested a biological risk of manure in spreading antimicrobial resistance and pathogenicity.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

The profile and persistence of clinically critical antibiotic resistance genes and human pathogenic bacteria in manure-amended farmland soils

Zhu

Lian

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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“…The selective pressure exerted by antimicrobials induces mechanisms for the acquisition of resistance in microorganisms, such as spot mutation or horizontal gene transfer, which pass from generation to generation and therefore select microorganisms that have inherited this resistance (9,10). Although several classes of broad-spectrum antibiotics are available to treat Gram-positive and Gram-negative bacterial infections, many pathogens rapidly evolve or acquire resistance to first-line treatments and respond only to last-resort antibiotics (11,12). An infection caused by resistant microorganisms is more difficult to treat.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance in the COVID-19 Landscape: Is There an Opportunity for Anti-Infective Antibodies and Antimicrobial Peptides?

et al. 2022

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Although COVID-19 has captured most of the public health attention, antimicrobial resistance (AMR) has not disappeared. To prevent the escape of resistant microorganisms in animals or environmental reservoirs a “one health approach” is desirable. In this context of COVID-19, AMR has probably been affected by the inappropriate or over-use of antibiotics. The increased use of antimicrobials and biocides for disinfection may have enhanced the prevalence of AMR. Antibiotics have been used empirically in patients with COVID-19 to avoid or prevent bacterial coinfection or superinfections. On the other hand, the measures to prevent the transmission of COVID-19 could have reduced the risk of the emergence of multidrug-resistant microorganisms. Since we do not currently have a sterilizing vaccine against SARS-CoV-2, the virus may still multiply in the organism and new mutations may occur. As a consequence, there is a risk of the appearance of new variants. Nature-derived anti-infective agents, such as antibodies and antimicrobial peptides (AMPs), are very promising in the fight against infectious diseases, because they are less likely to develop resistance, even though further investigation is still required.

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“…The de novo assembly of genomes based on long/ short reads was proceeded by SMRT Link and the Unicycle assembler, and subsequent error correction was conducted using Pilon or Arrow. 32 annotated using the Glimmer3 40 and the RAST server, 41 and the maps of plasmids were generated using the Snapgene server. Antibiotic-resistant genes were checked in the CARD database (https://card.mcmaster.ca/).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The minimal inhibitory concentrations (MICs) of six antibiotics (tetracycline, ampicillin, gentamicin, sulfamethoxazole, trimethoprim, and chloramphenicol) were determined by a liquid broth dilution method using the Mueller–Hinton medium. The evaluation of sensitivity or resistance was determined according to the corresponding criteria by the Clinical and Laboratory Standards Institute (CLSI) E.…”

Section: Methodsmentioning

confidence: 99%

Deciphering Multidrug-Resistant Plasmids in Disinfection Residual Bacteria from a Wastewater Treatment Plant

Lin,

Zhou,

Shuai

et al. 2024

Environ. Sci. Technol.

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Current disinfection processes pose an emerging environmental risk due to the ineffective removal of antibioticresistant bacteria, especially disinfection residual bacteria (DRB) carrying multidrug-resistant plasmids (MRPs). However, the characteristics of DRB-carried MRPs are poorly understood. In this study, qPCR analysis reveals that the total absolute abundance of four plasmids in postdisinfection effluent decreases by 1.15 log units, while their relative abundance increases by 0.11 copies/cell compared to investigated wastewater treatment plant (WWTP) influent. We obtain three distinctive DRB-carried MRPs (pWWTP-01−03) from postdisinfection effluent, each carrying 9−11 antibiotic-resistant genes (ARGs). pWWTP-01 contains all 11 ARGs within an ∼25 Kbp chimeric genomic island showing strong patterns of recombination with MRPs from foodborne outbreaks and hospitals. Antibiotic-, disinfectant-, and heavy-metal-resistant genes on the same plasmid underscore the potential roles of disinfectants and heavy metals in the coselection of ARGs. Additionally, pWWTP-02 harbors an adhesin-type virulence operon, implying risks of both antibiotic resistance and pathogenicity upon entering environments. Furthermore, some MRPs from DRB are capable of transferring and could confer selective advantages to recipients under environmentally relevant antibiotic pressure. Overall, this study advances our understanding of DRB-carried MRPs and highlights the imminent need to monitor and control wastewater MRPs for environmental security.

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Landscape of genes in hospital wastewater breaking through the defense line of last-resort antibiotics

Cited by 20 publications

References 48 publications

The profile and persistence of clinically critical antibiotic resistance genes and human pathogenic bacteria in manure-amended farmland soils

The profile and persistence of clinically critical antibiotic resistance genes and human pathogenic bacteria in manure-amended farmland soils

Antimicrobial Resistance in the COVID-19 Landscape: Is There an Opportunity for Anti-Infective Antibodies and Antimicrobial Peptides?

Deciphering Multidrug-Resistant Plasmids in Disinfection Residual Bacteria from a Wastewater Treatment Plant

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