Laminin Synthesis and the Adhesion Characteristics of Immortalized Human Corneal Epithelial Cells to Laminin Isoforms

Filenius, Sissi; Hormia, M; Rissanen, Jan; Burgeson, Robert E.; Yamada, Y.; Araki-Sasaki, Kaoru; Nakamura, Masatsugu; Virtanen, Ismo; Tervo, Timo

doi:10.1006/exer.2000.0933

Cited by 28 publications

(14 citation statements)

References 61 publications

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“…In our study, neither the epithelial BM nor the DM were immunoreactive for LM 1 chain, in agreement with Filenius et al (2001) and our previous data showing the disappearance of this chain from the epithelial BM of the human cornea after 12 wg and from the DM after 17 wg (Byström et al 2006). We were not able to detect any speciWc staining in the adult human cornea using a polyclonal antibody against LM 1 chain (Tiger et al 1997), although this antibody worked well in fetal corneal specimens (Byström et al 2006).…”

Section: Laminin 1 Chainsupporting

confidence: 91%

Laminins in normal, keratoconus, bullous keratopathy and scarred human corneas

Byström

Virtanen

Rousselle

et al. 2007

Histochem Cell Biol

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The laminin composition (LMalpha1-alpha5, beta1-beta3, gamma1 and gamma2 chains) of normal corneas and corneal buttons from keratoconus, bullous keratopathy (BKP), Fuchs' dystrophy + BKP, Fuchs' dystrophy without BKP and scar after deep lamellar keratoplasty (DLKP) was investigated with immunohistochemistry. The epithelial basement membranes (BMs) of both normal and diseased corneas contained LMalpha3, alpha5, beta1, beta3, gamma1 and gamma2 chains. The epithelial BM morphology was altered in the different diseases. Scarring was associated with irregular BM and ectopic stromal localization of different laminin chains. The Descemet's membrane (DM) contained LMalpha5, beta1 and gamma1 chains in all cases and additionally LMbeta3 and gamma2 chains in the majority of keratoconus corneas. The interface in the DLKP cornea had patches of LMalpha3, alpha4, alpha5, beta1 and beta2 chains, and an extra BM-like structure under the Bowman's membrane. These results suggest that laminin chains participate in the process of corneal scarring and in the pathogenesis of some corneal diseases. The novel finding of LMalpha3, beta3 and gamma2 in the DM of keratoconus buttons indicates that this membrane is also involved in the disease and that some cases of keratoconus may have a congenital origin, without normal downregulation of the LMbeta3 chain.

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Section: Laminin 1 Chainsupporting

confidence: 91%

Laminins in normal, keratoconus, bullous keratopathy and scarred human corneas

Byström

Virtanen

Rousselle

et al. 2007

Histochem Cell Biol

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“…21 Moreover, a recent study demonstrated that the adhesion of HCEC to plain plastic dishes was also abolished by monoclonal integrin a 3 b 1 antibodies, because HCEC newly secreted endogenous laminin-5, the main ligand for integrin a 3 b 1 . 22 We found that the cells grown in the presence of antibody against integrin a 3 b 1 decreased in cell number, but were not blocked at the G 0 /G 1 phase of the cell cycle. Our data is supported by the results that antibody against integrin a 3 b 1 also inhibited the cell number of the breast epithelial cells, but did not block the growth at a particular stage in the cell cycle.…”

Section: Discussionmentioning

confidence: 96%

Prolonged exposure to high glucose impaired cellular behavior of normal human corneal epithelial cells

Fujita¹,

Morita²,

Takase³

et al. 2003

Current Eye Research

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“…Adhesion and, in consequence, migration and proliferation depend on the function of integrin receptors. We found that the epithelial cells from the anterior capsule expressed β1α3, which is a receptor for a variety of extracellular matrix proteins including laminin and collagen IV [5,8]. Because the distribution of these integrin subunits was strongly impaired by treatment with Mibefradil we believe that inhibition of adhesion is significantly caused by the effect on β1α3 integrins.…”

Section: Discussionmentioning

confidence: 98%