Laminin is the ECM niche for trophoblast stem cells

Kiyozumi, Daiji; Nakano, Itsuko; Sato-Nishiuchi, Ryoko; Tanaka, Satoshi; Sekiguchi, Kiyotoshi

doi:10.26508/lsa.201900515

Cited by 22 publications

(19 citation statements)

References 42 publications

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“…We isolated ICMs for explant culture by immunosurgical removal of trophectoderm ( Solter and Knowles, 1975 ). ICMs were maintained intact and plated on dishes coated with laminin 111-E8 ( Kiyozumi et al., 2020 ) in N2B27 with or without PD+A83. In N2B27, a central mass of relatively undifferentiated cells persisted in most cases, but patches of trophectoderm morphology often outgrew.…”

Section: Resultsmentioning

confidence: 99%

Human naive epiblast cells possess unrestricted lineage potential

et al. 2021

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Summary Classic embryological experiments have established that the early mouse embryo develops via sequential lineage bifurcations. The first segregated lineage is the trophectoderm, essential for blastocyst formation. Mouse naive epiblast and derivative embryonic stem cells are restricted accordingly from producing trophectoderm. Here we show, in contrast, that human naive embryonic stem cells readily make blastocyst trophectoderm and descendant trophoblast cell types. Trophectoderm was induced rapidly and efficiently by inhibition of ERK/mitogen-activated protein kinase (MAPK) and Nodal signaling. Transcriptome comparison with the human embryo substantiated direct formation of trophectoderm with subsequent differentiation into syncytiotrophoblast, cytotrophoblast, and downstream trophoblast stem cells. During pluripotency progression lineage potential switches from trophectoderm to amnion. Live-cell tracking revealed that epiblast cells in the human blastocyst are also able to produce trophectoderm. Thus, the paradigm of developmental specification coupled to lineage restriction does not apply to humans. Instead, epiblast plasticity and the potential for blastocyst regeneration are retained until implantation.

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Section: Resultsmentioning

confidence: 99%

Human naive epiblast cells possess unrestricted lineage potential

et al. 2021

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“…In particular, laminin LAMA1 was highly expressed in the first trimester, whereas LAMB3, LAMA2, LAMC3 were upregulated in the second trimester. Laminins have an important role during implantation ( Haouzi et al, 2011 ), maintenance of trophoblast stemness ( Kiyozumi et al, 2020 ) and EVT migration ( Zhang et al, 2018b ).…”

Section: Resultsmentioning

confidence: 99%

RNA-Seq reveals changes in human placental metabolism, transport and endocrinology across the first–second trimester transition

et al. 2021

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The human placenta is exposed to major environmental changes towards the end of the first trimester associated with full onset of the maternal arterial placental circulation. Changes include a switch from histotrophic to hemotrophic nutrition, and a threefold rise in the intraplacental oxygen concentration. We evaluated their impact on trophoblast development and function using RNA-sequencing (RNA-Seq) and DNA-methylation analyses performed on the same chorionic villous samples at 7–8 (n=8) and 13–14 (n=6) weeks of gestation. Reads were adjusted for fetal sex. Most DEGs were associated with protein processing in the endoplasmic reticulum (ER), hormone secretion, transport, extracellular matrix, vasculogenesis, and reactive oxygen species metabolism. Transcripts higher in the first trimester were associated with synthesis and ER processing of peptide hormones, and glycolytic pathways. Transcripts encoding proteins mediating transport of oxygen, lipids, protein, glucose, and ions were significantly increased in the second trimester. The motifs of CBX3 and BCL6 were significantly overrepresented, indicating the involvement of these transcription factor networks in the regulation of trophoblast migration, proliferation and fusion. These findings are consistent with a high level of cell proliferation and hormone secretion by the early placenta to secure implantation in a physiological low-oxygen environment.

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“…Nevertheless, laminins mainly dictate the structure and assembly of MB [77]. Moreover, these glycoproteins play a crucial role in cell migration, adhesion, differentiation, and wound healing [78][79][80][81]. They also participate in early embryonic development and organogenesis.…”

Section: Lamininsmentioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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Laminin is the ECM niche for trophoblast stem cells

Cited by 22 publications

References 42 publications

Human naive epiblast cells possess unrestricted lineage potential

Human naive epiblast cells possess unrestricted lineage potential

RNA-Seq reveals changes in human placental metabolism, transport and endocrinology across the first–second trimester transition

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

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