Laminin induces formation of neurite-like processes and potentiates prolactin secretion by GH3 rat pituitary cells

Carvalho, Nicole; Picart, R; Moortele, Solange Van de; Tougard, Claude; Tixier‐Vidal, A.

doi:10.1111/j.1432-0436.1989.tb00820.x

Cited by 15 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the PRLproducing cell proliferation activities in vivo and in vitro systems may differ. The difference between cell proliferation in in vivo and in vitro systems PRL-CELL DIFFERENTIATION AND CELL CYCLE 731 was also observed in a study of the mammosomatotrophic cell line GH3 [13]. This study showed that GH3 tumor cell growth was dependent on systemic circulating estrogen in vivo, whereas estradiol did not stimulate GH3 cell proliferation in vitro.…”

Section: Brdu Labeling During Cell Differentiationsupporting

confidence: 53%

Prolactin-Producing Cells Differentiate from G0/G1-Arrested Somatotrophs In Vitro: An Analysis of Cell Cycle Phases and Mammotroph Differentiation.

et al. 1998

View full text Add to dashboard Cite

Abstract.In order to analyze the relationship between cell proliferation and mammotroph differentiation,we studied a somatotrophic cell line, MtT/S. MtT/S cell is known to differentiate into PRL-producing cells in response to stimulation with insulin or insulin-like growth factor-1 (IGF-1). Double immunostaining for bromodeoxyuridine (BrdU), which labels proliferating cells, and for GH or PRL showed that most BrdU-labeled cells were GH-immunopositive, whereas considerably few PRLpositive cells were labeled with BrdU. This was confirmed by immunostaining of proliferating cells with antibody to proliferating cell nuclear antigen (PCNA). Furthermore, flow-cytometry analysis indicated that most of the PRL-producing cells were in the GO/G1 phase of the cell cycle. In order to determine whether cell cycle changes are required for transdifferantiation of PRL-producing cells, MtT/S cells were cultivated in serum restricted medium for 7 days to reduce their mitotic activity and then treated with insulin and epidermal growth factor (EGF). Under these conditions, the cell cycle of MtT/S cells was significantly delayed, but the percentage of PRL-producing cells induced was almost identical to that under control conditions, showing that mitosis is not required for PRL-producing cell differentiation. We also labeled MtT/S cells with BrdU for 24 h during PRL-producing cell induction by insulin and EGF, and as a result BrdU-labeled proliferative cells were specifically absent from PRL-producing cell populations. These data, taken as whole, suggest that PRL cells differentiated from GO/G1 arrested somatotrophs and the PRL cells which appeared had their cell proliferation activity significantly declined. In conclusion, this is the first report showing the relationship cell between proliferation and differentiation of PRL cells.

show abstract

Section: Brdu Labeling During Cell Differentiationsupporting

confidence: 53%

Prolactin-Producing Cells Differentiate from G0/G1-Arrested Somatotrophs In Vitro: An Analysis of Cell Cycle Phases and Mammotroph Differentiation.

et al. 1998

View full text Add to dashboard Cite

show abstract

“…In the pituitary gland, the cytoskeletal network plays a crucial role in regulating the function of signal transduction. It has been suggested that low levels of filamin A, a cytoskeletal-associated protein, could represent a post-receptor mechanism of pharmacological resistance in pituitary adenomas, as this scaffolding protein stabilizes the SSTR2 and dopamine 2 receptor, linking them to their intracellular effectors [ 69 , 70 ]. We validated direct interactions of AIP with two isotypes of tubulins: TUBB, widely expressed, and TUBB2A, the major isotype in neural tissue [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

Multi-chaperone function modulation and association with cytoskeletal proteins are key features of the function of AIP in the pituitary gland

et al. 2018

View full text Add to dashboard Cite

Despite the well-recognized role of loss-of-function mutations of the aryl hydrocarbon receptor interacting protein gene (AIP) predisposing to pituitary adenomas, the pituitary-specific function of this tumor suppressor remains an enigma. To determine the repertoire of interacting partners for the AIP protein in somatotroph cells, wild-type and variant AIP proteins were used for pull-down/quantitative mass spectrometry experiments against lysates of rat somatotropinoma-derived cells; relevant findings were validated by co-immunoprecipitation and co-localization. Global gene expression was studied in AIP mutation positive and negative pituitary adenomas via RNA microarrays. Direct interaction with AIP was confirmed for three known and six novel partner proteins. Novel interactions with HSPA5 and HSPA9, together with known interactions with HSP90AA1, HSP90AB1 and HSPA8, indicate that the function/stability of multiple chaperone client proteins could be perturbed by a deficient AIP co-chaperone function. Interactions with TUBB, TUBB2A, NME1 and SOD1 were also identified. The AIP variants p.R304* and p.R304Q showed impaired interactions with HSPA8, HSP90AB1, NME1 and SOD1; p.R304* also displayed reduced binding to TUBB and TUBB2A, and AIP-mutated tumors showed reduced TUBB2A expression. Our findings suggest that cytoskeletal organization, cell motility/adhesion, as well as oxidative stress responses, are functions that are likely to be involved in the tumor suppressor activity of AIP.

show abstract

Laminin decreases PRL and IGFBP‐1 expression during in vitro decidualization of human endometrial stromal cells

Brar

Frank

Richards

et al. 1995

Journal Cellular Physiology

View full text Add to dashboard Cite

The expression of laminin, a major constituent of endometrial cell basement membranes, is increased during differentiation of human endometrial stromal cells (decidualization). To determine whether laminin plays a role in decidualization, we studied the effects of laminin substrate on the synthesis and release of prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1), two major secretory proteins of decidualized stromal cells. Endometrial stromal cells were plated on laminin as well as several other extracellular matrix (ECM) proteins (types 1 and IV collagen or fibronectin) and on plastic, and cultured in media containing medroxyprogesterone acetate (MPA) and estradiol. Cells cultured on plastic or ECM proteins displayed similar morphological changes indicative of decidualization. However, the release of PRL and IGFBP-1 from cells cultured on plastic and ECM proteins (types 1 and IV collagen and fibronection) was approximately 2.1-fold and 2.8-fold greater respectively, than from cells cultured on laminin. The decrease in PRL and IGFBP-1 expression in cells cultured on laminin was not due to differences in initial cell attachment efficiency or final DNA content. In addition, laminin had no effect on the content of laminin protein or fibronectin mRNA levels, indicating that the effects of laminin on PRL and IGFBP-1 were specific. PGE2 stimulated the release of PRL and IGFBP-1 from cells cultured on laminin to levels comparable to those from cells cultured on plastic or other ECM proteins. This indicates that the decrease in PRL and IGFBP-1 release by laminin was not due to a generalized unresponsiveness. In contrast to the effects of laminin during decidualization, PRL expression was not altered by laminin in terminally differentiated decidual cells isolated at term. Our results support a role for laminin in selectively regulating PRL and IGFBP-1 gene expression during in vitro decidualization of human endometrial stromal cells.

show abstract

Laminin induces formation of neurite-like processes and potentiates prolactin secretion by GH3 rat pituitary cells

Cited by 15 publications

References 39 publications

Prolactin-Producing Cells Differentiate from G0/G1-Arrested Somatotrophs In Vitro: An Analysis of Cell Cycle Phases and Mammotroph Differentiation.

Prolactin-Producing Cells Differentiate from G0/G1-Arrested Somatotrophs In Vitro: An Analysis of Cell Cycle Phases and Mammotroph Differentiation.

Multi-chaperone function modulation and association with cytoskeletal proteins are key features of the function of AIP in the pituitary gland

Laminin decreases PRL and IGFBP‐1 expression during in vitro decidualization of human endometrial stromal cells

Contact Info

Product

Resources

About