Laminin Expression in Colorectal Carcinomas Varying in Degree of Differentiation

Daneker, George W.; Mercurio, Arthur M.; Guerra, Lisa; Wolf, Barbara C.; Salem, Ronald R.; Bägli, Darius; Steele, Glenn

doi:10.1001/archsurg.1987.01400240118022

Cited by 43 publications

(19 citation statements)

References 15 publications

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“…This highly tumorigenic capacity and the greater capacity to invade [5,6], correlates with in vitro studies. Poorly differentiated cell lines showed a higher rate of movement through synthetic basement membranes, reduced laminin production and increased laminin receptor expression [5,18]. Patients with poorly differentiated colorectal cancers generally have a worse prognosis due to a higher incidence of local and distant recurrence [3,4].…”

Section: Discussionmentioning

confidence: 99%

Metastatic potential of human colon cancer cell lines: relationship to cellular differentiation and carcinoembryonic antigen production

et al. 1992

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The relationship between cellular differentiation and carcinoembryonic antigen (CEA) production by human colorectal tumor cells and their ability to form hepatic metastases was studied. Eight human colon cancer cell lines were injected into athymic mice using different routes of administration to characterize their metastatic potential. The four poorly differentiated, non or low CEA producing cell lines were poorly metastatic to the liver after intrasplenic injection. After intraperitoneal implantation the same cell lines were highly tumorigenic, and subsequently metastatic to the liver. In contrast, the four moderate to well-differentiated cell lines that produced moderate to high levels of CEA were highly metastatic to the liver following intrasplenic injection. After intraperitoneal implantation they were less tumorigenic, and metastatic to the liver. We conclude that in this system poorly differentiated non or low CEA producing colorectal cell lines have a lower metastatic capacity compared to the well-differentiated high CEA producing colorectal cell lines. These data correlate directly with the pattern of metastatic spread and clinical course observed in patients with these tumors, suggesting that degree of differentiation and level of CEA production may play a role in development of site-specific metastases.

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Section: Discussionmentioning

confidence: 99%

Metastatic potential of human colon cancer cell lines: relationship to cellular differentiation and carcinoembryonic antigen production

et al. 1992

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“…Several lines of evidences suggest that basement membranes are involved in cell adhesion, migration, polarization, and differentiation (Bernfield et al 1984;Timpl and Dziadek 1986;Sanders 1987;Rodriguez-Boulan and Nelson 1989). Of interest is the strong correlation that exists between the level of alteration of the basement membrane and the degree of malignancy in colorectal cancers (Burtin et al 1982;Daneker et al 1987;Havenith et al 1988). However, it is not yet clear whether the influence provided by the presence of these components on intestinal cytodifferentiation is inductive or only permissive Hahn 1988).…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of extracellular matrix components during organogenesis in the human small intestine

1991

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The expression and distribution of several major extracellular matrix macromolecules were investigated at the epithelial-mesenchymal interface of the human fetal small intestine from 8 to 20 weeks of gestation. Localization of heparan sulfate proteoglycan, type-IV collagen and laminin, three basement membrane components, as well as fibronectin and tenascin, were assessed by indirect immunofluorescence staining on cryostat sections, and correlated to morphogenesis and epithelial cell differentiation. Basement membrane components and fibronectin were all detected as early as 8 weeks (a time when the epithelium is still stratified and does not express sucrase-isomaltase). Tenascin appeared only after short villi had developed (around 10 weeks) and was restricted to the connective tissue at the tip of villus rudiments. At 18 weeks, well-formed villi and crypts were apparent. The antibody against heparan sulfate proteoglycan stained exclusively the epithelial basement membrane. Anti-type-IV collagen and anti-laminin antibodies stained the epithelial basement membrane and also cellular and fibrillar structures in the lamina propria. Fibronectin was found uniformly distributed over the lamina propria except in the upper third position of the villus core. On the contrary tenascin was mainly localized in the stroma at the tip of the villi. Staining for tenascin was also detected at the epithelial-mesenchymal interface of the villus and in the mesenchyme immediately surrounding budding crypts. These results provide basic data concerning the development of the human gut, and suggest that extracellular matrix components could be involved in the remodelling process of the intestinal mucosa.

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“…Recent observations of colorectal carcinomas disclosed that it was not the laminin synthesis but rather the laminin secretion rates which appeared to be directly related to the degree of differentiation (Daneker et al, 1987). Both well-differentiated and poorly-differentiated carcinoma cells are able to synthesize basement membrane glycoproteins, at similar rates.…”

Section: Discussionmentioning

confidence: 99%

Distribution of basement membrane antigens in human esophageal lesions: An immunohistochemical study

Mori

Shimono

Kido

et al. 1991

Intl Journal of Cancer

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The distribution of basement membrane components, type-IV collagen and laminin, was studied immunohistochemically in human samples of normal, hyperplastic, dysplastic and carcinomatous esophageal tissue. The expression of basement membrane components in normal, hyperplastic, and mildly and moderately dysplastic mucosa was characterized by a thick, continuous and linear staining pattern. In severe dysplasia and carcinoma in situ, the basement membrane was thinner and occasionally discontinuous. The distribution of basement membrane in invasive carcinomas varied greatly; well-differentiated, cohesive tumors had a basement membrane, however, poorly-differentiated tumors showing irregular cords, or individual cell infiltration, lacked this membrane at the tumor-stromal interface. Many moderately-differentiated tumors showed probable immature assembly of basement membrane components. The expression of basement membrane was also influenced by the extent of stromal inflammation. The absence or presence (staining pattern) of basement membrane components in esophageal squamous-cell carcinoma did not correlate with the survival rate, but did correlate with the histologic differentiation of epithelial organization.

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Laminin Expression in Colorectal Carcinomas Varying in Degree of Differentiation

Cited by 43 publications

References 15 publications

Metastatic potential of human colon cancer cell lines: relationship to cellular differentiation and carcinoembryonic antigen production

Metastatic potential of human colon cancer cell lines: relationship to cellular differentiation and carcinoembryonic antigen production

Immunolocalization of extracellular matrix components during organogenesis in the human small intestine

Distribution of basement membrane antigens in human esophageal lesions: An immunohistochemical study

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