Laminin 5 processing and its integration into the ECM

Integrin Regulation of Epidermal Functions in Wounds

“…64,134 Laminin-332 proteolysis is also likely to be an important mode of regulating BM architecture through modulation of key linkages with other ECM proteins, such as the interaction of fibulin-2 with laminin-332 described above. 58 Indeed, the L4 module to which fibulin-2 binds is lost upon proteolytic processing of c 2 , which might be an important step for BM maturation. 128,129,132 Interestingly, recent data from our laboratory indicates a role for epidermal a 3 b 1 in the regulation of laminin-c 2 chain processing, both in vivo during wound healing and in keratinocytes cultured under conditions of high calcium.…”

Blockade of β1 Integrin–Laminin-5 Interaction Affects Spreading and Insulin Secretion of Rat β-Cells Attached on Extracellular Matrix

“…We have shown that isolated rat islet ␤-cells cultured on the matrix produced by 804G cells (referred to as 804G matrix) secrete twofold more insulin in response to glucose compared with cells cultured on plastic or poly-L-lysine (22,23). This matrix, deposited by 804G rat bladder carcinoma cells, is rich in laminin-5 (24 -26), a heterotrimer protein consisting of ␣3, ␤3, and ␥2 subunits, and an essential component of several epithelial basement membranes (27,28). Our previous study suggests that the effects of 804G matrix on ␤-cells are mediated, at least in part, by laminin-5 (22).…”

Cytoplasmic and stromal expression of laminin γ 2 chain correlates with infiltrative invasion in ovarian mucinous neoplasms of gastro-intestinal type

“…In well differentiated infiltrative invasive carcinoma, various tumor invasion stimulators such as MMP or BMP-1 may function, then fragments of Á 2 chain may express in the tumor stroma. The resultant laminin 5 molecule would be stabilized in the extracellular matrix (36), and this can explain the basement membranous Á 2 chain identified in well differentiated infiltrative invasive carcinoma.…”