Laminin-332 regulates differentiation of human interfollicular epidermal stem cells

Yamada, Takaaki; Hasegawa, Seiji; Miyachi, Katsuma; Date, Yasushi; Inoue, Yu; Yagami, Akiko; Arima, Masaru; Iwata, Y.; Yamamoto, Naoki; Nakata, Satoshi; Matsunaga, Kayoko; Sugiura, Kazumitsu; Akamatsu, Hirohiko

doi:10.1016/j.mad.2018.03.007

Cited by 27 publications

(28 citation statements)

References 34 publications

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“…Interestingly, expression of the laminin protein was only observed in the uppermost layer of the dermal tissue and blood vessels. While the source of the laminin protein is epidermal keratinocytes, after expression, laminin is assembled with other basement membrane proteins and observed at the tips of dermal papillae, which is consistent with previous reports [20]. Similar increases of nidogen proteins in the uppermost layer of the dermal tissue were also observed.…”

Section: Resultssupporting

confidence: 92%

“…While five α, four β and three γ chains are currently identified in mammals, only 16 different isoforms are observed in mammals. Recently, the important roles of laminin 522 during the differentiation of interfollicular stem cells and the establishment of proper structures in an in vitro 3D culture model were reported [20]. Also, the pentapeptide derived from the laminin b1 chain, tyrosine-isoleucine-glycine-serine-arginine (YIGSR), was shown to stimulate collagen synthesis in cultured human dermal fibroblasts [29], which also suggests the possible anti-aging effects of laminin-stimulating ingredients.…”

Section: Discussionmentioning

confidence: 97%

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Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression

Jeong

Yoon

Kim

et al. 2019

IJMS

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The dermal-epidermal junction (DEJ) provides a physical and biological interface between the epidermis and the dermis. In addition to providing a structural integrity, the DEJ also acts as a passageway for molecular transport. Based on the recently reported importance of the DEJ in skin aging, novel peptide derivatives have been tested for their effects on basement membrane (BM) protein expressions in cultured human epidermal keratinocytes. As a result, protein expressions of collagen XVII, laminin and nidogen were stimulated by the test peptide and peptides complex. Further ex vivo evaluation using excised human skin, confirmed that the topical application of the peptides complex significantly increased dermal collagen expression, as well as expressions of collagen XVII and laminin. Interestingly, while the origin of the laminin protein is epidermal keratinocytes, the immunohistochemical staining of skin showed that laminin was only detected in the uppermost layer of the dermis, which suggests a tight assembly of laminin protein onto the dermal side of the DEJ. These results suggest that a peptide complex could improve the structural properties of the DEJ through its ability to stimulate BM proteins. In order to evaluate the anti-wrinkle benefits of the peptide complex in vivo, a clinical study was performed on 22 healthy Asian female volunteers older than 40 years. As a result, significant improvements in skin wrinkles for all of the five sites were observed after two weeks, as assessed by skin topographic measurements. Collectively, these results demonstrate the anti-aging efficacy of the peptides complex.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 97%

Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression

Jeong

Yoon

Kim

et al. 2019

IJMS

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“…It has been reported that global genomic DNA methylation is reduced in the epidermis of sun‐exposed areas compared to that of sun‐protected areas . To investigate whether UV exposure induces global DNA hypomethylation and WNT1 expression in vitro, we used HDK1 cells, an immortalized cell line established from normal human epidermal keratinocytes (NHEKs); at an early passage, NHEKs have proliferation and differentiation potentials and thus are considered an IFE‐SC‐rich population . First, these cells were irradiated with UVB of 10 mJ/cm 2 or 100 mJ/cm 2 at a time.…”

Section: Resultsmentioning

confidence: 99%

“…Human immortalized keratinocytes (HDK1 cells), an in vitro interfollicular epidermal stem cell model, were cultured in keratinocyte serum‐free medium (Life Technologies). One day after seeding onto 6‐well plates at a density of 1 × 10 4 cells/cm 2 , cells were irradiated with UVB by Toshiba FL‐20 SE fluorescent lamps (Toshiba Electric) at a dose of 10 mJ/cm 2 for 2 weeks (four times or fewer per week).…”

Section: Methodsmentioning

confidence: 99%

UV irradiation‐induced DNA hypomethylation around WNT1 gene: Implications for solar lentigines

Yamada

Hasegawa

Iwata

et al. 2019

Experimental Dermatology

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Wnt/β‐catenin signalling promotes melanogenesis in melanocytes and also induces melanocytogenesis from melanocyte stem cells (McSCs). Previous study reported that WNT1, a ligand which activates Wnt/β‐catenin signalling pathway, was more highly expressed in the epidermis at SLs than in normal skin areas, suggesting that WNT1 causes hyperpigmentation. To elucidate the mechanism by which WNT1 expression is increased in SLs, we examined the methylation of 5‐carbon of cytosine (5mC), that is 5‐methylcytosine (5mC) level, in a region within the WNT1 promoter; the methylation of the region was known to negatively regulate WNT1 gene expression. We used an immortalized cell line of human interfollicular epidermal stem cells to analyse the effect of UVB irradiation on DNA methylation level of WNT1 promoter and found that UVB irradiation caused demethylation of WNT1 promoter and promoted WNT1 mRNA expression. It was also found that UVB irradiation reduced the expression of DNA methyltransferase 1 (DNMT1), an enzyme responsible for maintaining methylation patterns during cell division. Pathological analysis of SLs and non‐SL regions in the human skin revealed that both DNMT1 expression and 5mC level were decreased at SLs compared to non‐SL skins. Furthermore, bisulphite sequencing showed that the methylated CpG level in WNT1 promoter was also lower at SLs than in non‐SL skins. Thus, in the skin exposed to a high amount of UV rays, excessive expression of WNT1 is thought to be caused by the demethylation of WNT1 promoter, and the upregulated WNT1 promotes melanocytogenesis and melanogenesis, then resulting in SL formation.

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“…Fibrin matrices have been used for the culture of several cell types, especially keratinocytes and fibroblasts, and reported to enhance cell proliferation (Sese, Cole, & Tawil, 2011). Moreover, it has been shown that the presence of basement membrane proteins such as Laminin 332 (Yamada et al, 2018), Collagen IV (Segal et al, 2008), or Perlecan (Nakamura & Tokura, 2011) would be favorable to the proliferation of keratinocyte. In our study, we showed that the use of fibrin matrices (hPBM and FPF) enhanced the deposition and the preservation of basement membrane proteins and favored basal keratinocyte proliferation, compared with cultures with no matrix (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Influence of fibrin matrices and their released factors on epidermal substitute phenotype and engraftment

Alexaline

Magne

Rodríguez

et al. 2019

J Tissue Eng Regen Med

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SUMMARY Cultured epithelial autografts (CEAs) represent a life‐saving surgical technique for full‐thickness skin burns covering more than 60% total body surface area. However, CEAs present numerous drawbacks leading to heavy cosmetic and functional sequelae. In our previous study, we showed that human plasma‐based fibrin matrices (hPBM) could improve the reparative potential of CEAs. Therefore, in the present work, we sought to investigate the role of hPBM compared with fibrin from purified fibrinogen (FPF) or plastic support on epidermal substitute formation and engraftment. The use of hPBM for epidermal substitute culture improved keratinocyte migration, proliferation, and epidermal substitute organization to a better extent than FPF in vitro. Both fibrin matrices favored greater dermal–epidermal junction protein deposition and prevented their degradation. Keratinocyte differentiation was also decreased using both fibrin matrices. Basement membrane protein deposition was mainly influenced by matrix whereas growth factors released from fibrin especially by hPBM were shown to enhance in vitro keratinocyte migration, proliferation, and epidermal substitute organization. Ultimately, epidermal substitutes grown on hPBM displayed better engraftment rates than those cultured on FPF or on plastic support in a NOD‐SCID model of acute wound with the formation of a functional dermal–epidermal junction. Together, these results show the positive impact of fibrin matrices and their released growth factor on epidermal substitute phenotype and grafting efficiency. Fibrin matrices, and especially hPBM, may therefore be of interest to favor the treatment of full‐thickness burn patients.

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Laminin-332 regulates differentiation of human interfollicular epidermal stem cells

Cited by 27 publications

References 34 publications

Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression

Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression

UV irradiation‐induced DNA hypomethylation around WNT1 gene: Implications for solar lentigines

Influence of fibrin matrices and their released factors on epidermal substitute phenotype and engraftment

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