Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering

Kwon, Gi-Wan; Gupta, K. C.; Jung, Ki-Hyun; Kang, Inn‐Kyu

doi:10.1186/s40824-017-0097-3

Cited by 30 publications

(14 citation statements)

References 41 publications

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“…For the preparation of the PCL fibrous matrix, PCL solution (10 mL, 10 wt%, dissolved in CF:TFE = 8:2) was electrospun onto the aluminum dish using a syringe pump (NE-1000, New Era Pump Systems Inc., New York, USA). A high voltage was applied between the spinneret (a 24 G needle) and the collector located 15 cm away [ 24 ]. The syringe was horizontally fixed on the syringe pump and the PCL and ND/PCL solutions were electrospun onto an aluminum foil collector.…”

Section: Methodsmentioning

confidence: 99%

Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues

et al. 2018

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BackgroundElectrospun fibrous matrices are of great importance for tissue engineering and drug delivery device. However, relatively low mechanical strength of the fibrous matrix is one of the major disadvantages. NDs with a positive charge were selected to enhance the mechanical property of a composited fibrous matrix by inducing the intermolecular interaction between NDs and polymer chain. We prepared ND-composited poly (ε-caprolactone) (PCL) fibrous matrices by electrospinning and evaluated their performance in terms of mechanical strength and cell behaviors.MethodsA predetermined amounts of NDs (0.5, 1, 2 and 3 wt%) were added into PCL solution in a mixture of chloroform and 2,2,2-trifluoroethanol (8:2). ND-composited PCL (ND/PCL) fibrous matrices were prepared by electrospinning method. The tensile properties of the ND/PCL fibrous matrices were analyzed by using a universal testing machine. Mouse calvaria-derived preosteoblast (MC3T3-E1) was used for cell proliferation, alkaline phosphatase (ALP) assay, and Alizarin Red S staining.ResultsThe diameters of the fibrous matrices were adjusted to approximately 1.8 μm by changing process variables. The intermolecular interaction between NDs and PCL polymers resulted in the increased tensile strength and the favorable interfacial adhesion in the ND/PCL fibrous matrices. The ND/PCL fibrous matrix with 1 wt% of ND had the highest tensile strength among the samples and also improved proliferation and differentiation of MC3T3-E1 cells.ConclusionsCompared to the other samples, the ND/PCL fibrous matrix with 1 wt% of ND concentration exhibited superior performances for MC3T3 cells. The ND/PCL fibrous matrix can be potentially used for bone and dental tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues

et al. 2018

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“…Inorganic compounds such as hydroxyapatite among other calcium phosphates are frequently used for the fabrication of bone substitutes for tissue engineering purposes either in combination with polymers [24][25][26][27][28][29] or with collagen to mimic the natural bone composition [30][31][32][33][34][35]. In a recent work of Linh and co-authors, the surface of porous hydroxyapatite scaffolds was modified by collagen treatment and BMP-2 conjugation [36].…”

Section: Discussionmentioning

confidence: 99%

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

et al. 2020

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Extensive efforts were undertaken to develop suitable biomaterials for tissue engineering (TE) applications. To facilitate clinical approval processes and ensure the success of TE applications, bioinspired concepts are currently focused on. Working on bone tissue engineering, we describe in the present study a method for biofunctionalization of collagen/hydroxyapatite composites with BMP-2 mimetic peptides. This approach is expected to be fundamentally transferable to other tissue engineering fields. A modified BMP-2 mimetic peptide containing a negatively charged poly-glutamic acid residue (E7 BMP-2 peptide) was used to bind positively charged hydroxyapatite (HA) particles by electrostatic attraction. Binding efficiency was biochemically detected to be on average 85% compared to 30% of BMP-2 peptide without E7 residue. By quartz crystal microbalance (QCM) analysis, we could demonstrate the time-dependent dissociation of the BMP-2 mimetic peptides and the stable binding of the E7 BMP-2 peptides on HA-coated quartz crystals. As shown by immunofluorescence staining, alkaline phosphatase expression is similar to that detected in jaw periosteal cells (JPCs) stimulated with the whole BMP-2 protein. Mineralization potential of JPCs in the presence of BMP-2 mimetic peptides was also shown to be at least similar or significantly higher when low peptide concentrations were used, as compared to JPCs cultured in the presence of recombinant BMP-2 controls. In the following, collagen/hydroxyapatite composite materials were prepared. By proliferation analysis, we detected a decrease in cell viability with increasing HA ratios. Therefore, we chose a collagen/hydroxyapatite ratio of 1:2, similar to the natural composition of bone. The following inclusion of E7 BMP-2 peptides within the composite material resulted in significantly elevated long-term JPC proliferation under osteogenic conditions. We conclude that our advanced approach for fast and cost-effective scaffold preparation and biofunctionalization is suitable for improved and prolonged JPC proliferation. Further studies should prove the functionality of composite scaffolds in vivo.

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“…Specific cell responses depend on CP surface characteristics such as roughness, topography, surface free energy, charge, chemistry, and other properties such as electrical conductivity or mechanical actuation [26], [229]. In recent years, it also has been indicated that nanostructured biomaterials significantly affect the biological functions of cells [230], [231], [232], [233], [234]. Within this group of materials at the nanometre scale (nanofibres, nanotubes, nanoparticles, and nanofilaments) [234], [235], CPs may be applied, and their biological activity towards cells may be controlled by electrical stimulation [26], [236], [153], [237], [238], [239], [240].…”

Section: Main Assumptions Of Tissue Engineering and Icp Applications mentioning

confidence: 99%

Conducting Polymers, Hydrogels and Their Composites: Preparation, Properties and Bioapplications

Tomczykowa

Płońska‐Brzezińska

2019

Preprint

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This review is focused on current state-of-the-art research on electroactive-based materials and their synthesis, as well as their physicochemical and biological properties. Special attention is paid to pristine intrinsically conducting polymers (ICPs) and their composites with other organic and inorganic components, well-defined micro- and nanostructures, and enhanced surface areas compared with those of conventionally prepared ICPs. Hydrogels, due to their defined porous structures and being filled with aqueous solution, offer the ability to increase the amount of immobilized chemical, biological or biochemical molecules. When other components are incorporated into ICPs, the materials form composites; in this particular case, they form conductive composites. The design and synthesis of conductive composites result in the inheritance of the advantages of each component and offer new features because of the synergistic effects between the components. The resulting structures of ICPs, conducting polymer hydrogels and their composites, as well as the unusual physicochemical properties, biocompatibility and multi-functionality of these materials, facilitate their bioapplications. The synergistic effects between constituents have made these materials particularly attractive as sensing elements for biological agents, and they also enable the immobilization of bioreceptors such as enzymes, antigen–antibodies, and nucleic acids onto their surfaces for the detection of an array of biological agents. Currently, these materials have unlimited applicability in biomedicine. In this review, we have limited discussion to three areas in which it seems that the use of ICPs and materials, including their different forms, are particularly interesting, namely, biosensors, delivery of drugs and tissue engineering.

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Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering

Cited by 30 publications

References 41 publications

Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues

Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

Conducting Polymers, Hydrogels and Their Composites: Preparation, Properties and Bioapplications

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