Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering

Chen, Zhuoyue; Song, Yong; Zhang, Jing; Liu, Wei; Cui, Jihong; Li, Hongmin; Chen, Fulin

doi:10.1016/j.msec.2016.11.070

Cited by 69 publications

(64 citation statements)

References 46 publications

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“…The high porosity can increase the contact area between the cells and the scaffold, and it can also promote tissue growth in the material. The size of spread MSCs was 20–50 μ m. The pore size of scaffold (25–50 μ m) was big enough to allow cellular migration and infiltration [ 21 ]. Researchers have also confirmed that the suitable porosity of scaffolds for the penetration of cells is within the range of 60–90% [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Preparation of P3HB4HB/(Gelatin + PVA) Composite Scaffolds by Coaxial Electrospinning and Its Biocompatibility Evaluation

Liu

et al. 2017

BioMed Research International

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This study was conducted to prepare coaxial electrospun scaffolds of P3HB4HB/(gelatin + PVA) with various concentration ratios with P3HB4HB as the core solution and gelatin + PVA mixture as the shell solution; the mass ratios of gelatin and PVA in each 10 mL shell mixture were 0.6 g : 0.2 g (Group A), 0.4 g : 0.4 g (Group B), and 0.2 g : 0.6 g (Group C). The results showed that the pore size, porosity, and cell proliferation rate of Group C were better than those of Groups A and B. The ascending order of the tensile strength and modulus of elasticity was Group A < Group B < Group C. The surface roughness was Group C > Group B > Group A. The osteogenic and chondrogenic-specific staining showed that Group C was stronger than Groups A and B. This study demonstrates that when the mass ratio of gelatin : PVA was 0.2 g : 0.6 g, a P3HB4HB/(gelatin + PVA) composite scaffold with a core-shell structure can be prepared, and the scaffold has good biocompatibility that it may be an ideal scaffold for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Preparation of P3HB4HB/(Gelatin + PVA) Composite Scaffolds by Coaxial Electrospinning and Its Biocompatibility Evaluation

Liu

et al. 2017

BioMed Research International

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“…Meanwhile, multipotent stem cells could differentiate into specific cell lineages [16]. Human dental pulp stem cells with a potential of odontogenic and osteogenic differentiation are one of the sufficient and available sources for tissue engineering [17,18]. These cells have proved to be more available and shown higher cell proliferation compared to bone marrow mesenchymal stem cells (MSC) [19].…”

Section: Introductionmentioning

confidence: 99%

Towards osteogenic differentiation of human dental pulp stem cells on PCL-PEG-PCL/zeolite nanofibrous scaffolds

Alipour

Aghazadeh

Akbarzadeh

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Presently, tissue engineering has been developed as an effective option in the restoration and repair of tissue defects. One of the tissue engineering strategies is to use both biodegradable scaffolds and stimulating factors for enhancing cell responses. In this study, the effect of zeolite was assessed on cell viability, proliferation, osteo/odontogenic differentiation, and mineralization of human dental pulp stem cells (hDPSCs) cultured on poly (e-coprolactone)poly (ethylene glycol)-poly (e-caprolactone) (PCL-PEG-PCL) nanofibers. For this purpose, PCL-PEG-PCL nanofibrous scaffolds incorporated with zeolite were prepared via electrospinning. Both PCL-PEG-PCL and PCL-PEG-PCL/Zeolite nanofibrous scaffolds revealed bead-less constructions with average diameters of 430 nm and 437 nm, respectively. HDPSCs were transferred to PCL-PEG-PCL nanofibrous scaffolds containing zeolite nanoparticles. Cell adhesion and proliferation of hDPSCs and their osteo/odontogenic differentiation on these scaffolds were evaluated using MTT assay, Alizarin red S staining, and qRT-PCR assay. The results revealed that PCL-PEG-PCL/Zeolite nanofibrous scaffolds could support better cell adhesion, proliferation and osteogenic differentiation of hDPSCs and as such is expected to be a promising scaffold for bone tissue engineering applications.

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“…Titanium oxide (TiO 2 ) showed no effect on osteoblast growth when TiO 2 was added to PHB/HA scaffolds (34). The above mentioned studies also reported improved mechanical properties of PHB/HA scaffolds (3,11,24,32). Bioglass with osteoconduction and osteostimulation properties is another bioactive inorganic phase used in making composites with PHB.…”

Section: Physicochemical Modifications Of Phb In the Field Of Bone Timentioning

confidence: 87%

“…Wang et al (28,48) evaluated the attachment, proliferation and differentiation of rabbit bone marrow cells on PHB and PHB-HA scaffolds. PHB-HA scaffolds were shown to be a suitable biomaterial for rabbit bone marrow cell attachment, proliferation and differentiation (28,32,48). Several studies established in vitro tests of PHB scaffolds on murine osteoblasts (6,7,29).…”

Section: In Vitro Biocompatibility Of Phb On Bone Cellsmentioning

confidence: 99%

Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

Dariš

Knez

2019

Acta Pharmaceutica

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Poly(3-hydroxybutyrate) is a natural polymer, produced by different bacteria, with good biocompatibility and biode gradability. Cardiovascular patches, scaffolds in tissue engineering and drug carriers are some of the possible biomedical applications of poly(3-hydroxybutyrate). In the past decade, many researchers examined the different physicochemical modifications of poly(3-hydroxybutyrate) in order to improve its properties for use in the field of bone tissue engineering. Poly(3-hydroxybutyrate) composites with hydroxy apatite and bioglass are intensively tested with animal and human osteoblasts in vitro to provide information about their bio com patibility, biodegradability and osteoinductivity. Good bone regeneration was proven when poly(3-hydroxybutyrate) patches were implanted in vivo in bone tissue of cats, mini pigs and rats. This review summarizes the recent reports of in vitro and in vivo studies of pure poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate) composites with the emphasis on their bioactivity and biocompatibility with bone cells.

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Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering

Cited by 69 publications

References 46 publications

Preparation of P3HB4HB/(Gelatin + PVA) Composite Scaffolds by Coaxial Electrospinning and Its Biocompatibility Evaluation

Preparation of P3HB4HB/(Gelatin + PVA) Composite Scaffolds by Coaxial Electrospinning and Its Biocompatibility Evaluation

Towards osteogenic differentiation of human dental pulp stem cells on PCL-PEG-PCL/zeolite nanofibrous scaffolds

Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

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