Laminarin-induced apoptosis in human colon cancer LoVo cells

Abstract

A number of scientific studies have revealed that laminarin has antitumor effects. Therefore, the aim of the present study was to investigate the apoptosis of LoVo cells and the underlying mechanisms induced by laminarin. LoVo cells were treated with various concentrations of laminarin and fluorescence-inverted microscopy was used to observe the morphology of LoVo cells treated with laminarin. In addition, western blotting was performed to analyze the expression levels of death receptor (DR)4, DR5, TNF-related apoptosis-inducing ligand (TRAIL), Fas-associated protein with death domain (FADD), caspase-8, caspase-3, Bid and tBid. Flow cytometry was conducted to analyze the expressions of Bcl-2 and Bax, and spectrophotometry was performed to quantify the activity of caspases-8, -3, -6 and -7. Following the treatment of LoVo cells with laminarin for 24 h, the expression levels of DR4, DR5, TRAIL, FADD, Bid, tBid and Bax were observed to be upregulated, whereas the expression levels of pro-caspase-8, pro-caspase-3 and Bcl-2 were downregulated. In addition, the activities of casapse-8, -3, -6 and -7 were observed to increase, which was a significant difference when compared with those of the control group. Therefore, laminarin is considered to induce the apoptosis of LoVo cells, which may occur via a DR pathway, suggesting that laminarin may be a potent agent for cancer treatment.

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

“…In another human colon cell line HT-29, laminarin was proved to induce apoptosis of cancer cells by regulating the insulin-like growth fator-IR (IGF-1R) signaling pathway (107). In the same cell line LOVO, laminarin was demonstrated by another research group to increase apoptosis of cancer cells by activating the death receptor (DR) signaling pathway (108).…”

Laminarin from Seaweed (Laminaria japonica) Inhibits Hepatocellular Carcinoma Through Upregulating Senescence Marker Protein-30

“…The regulatory mechanisms of laminarin in tumors are complex and have been suggested to be associated with ErbB2 phosphorylation, mitochondria, and the death receptor pathway in CC cells. 9,10,21 In this study, the expression of a prognostic marker of HCC, SMP-30, was detected. We found that the treatment with laminarin significantly increased the expression of SMP-30 in Bel-7404 and HepG2 cells but not in LO2 cells, compared with the expression in the control cells.…”

Pharmacological, pharmaceutical, cosmetic and diagnostic applications of sulfated polysaccharides from marine algae and bacteria

“…Laminarin was found to increase the release of hydrogen peroxide, calcium, nitric oxide, monocyte chemotactic protein-1, vascular endothelial growth factor, leukemia inhibitory factor, and granulocyte-colony stimulating factor in RAW 264.7 cells (Lee et al, 2012). It is known to induce apoptosis in human colon cancer cells by multiple pathways (Ji and Ji, 2014). It demonstrated protective effect against cisplatin-induced ototoxicity (Han et al, 2016).…”