Laminar shear flow increases hydrogen sulfide and activates a nitric oxide producing signaling cascade in endothelial cells

Huang, Bin; Chen, Chang Ting; Chen, Chi Shia; Wang, Yun-Ming; Hsieh, Hsyue Jen; Wang, Danny Ling

doi:10.1016/j.bbrc.2015.07.115

Cited by 27 publications

(22 citation statements)

References 35 publications

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“…Indeed, placental total NOx was increased in placentae from Preg+NaHS and HTN‐Preg+NaHS when compared to Norm‐Preg. In recent studies, it has been proposed that H 2 S may modulate NO production and our results regarding placental NOx levels are in accordance with this idea. No significant differences were found between placental NOx levels from HTN‐Preg and HTN‐Preg+NaHS animals; however, the significant increases in HTN‐Preg+NaHS vs Norm‐Preg suggest that placental NO may partially contribute to the re‐establishment of placental efficiency in HTN‐Preg+NaHS animals.…”

Section: Discussionsupporting

confidence: 91%

“…When relaxation was triggered by ACh in the presence of NOS inhibitor L‐NAME, a greater relaxation was reached in aortic rings from HTN‐Preg+NaHS animals. Previous studies showed that H 2 S may increase expression and activity of endothelial NOS, thus generating excessive amounts of NO that could be responsible for the relaxation observed even in the presence of L‐NAME. Also, it has been described that H 2 S was responsible for NO formation under hypoxic conditions in a NOS‐independent manner, through the reduction of nitrite back to NO .…”

Section: Discussionmentioning

confidence: 99%

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Increases in placental nitric oxide, but not nitric oxide‐mediated relaxation, underlie the improvement in placental efficiency and antihypertensive effects of hydrogen sulphide donor in hypertensive pregnancy

Possomato-Vieira

Chimini

Silva

et al. 2018

Clin Exp Pharma Physio

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Dysregulation of hydrogen sulphide (H S) producing enzymes has been related to hypertensive pregnancy, and H S donor, sodium hydrosulphide (NaHS) exerts antihypertensive effects, modulates angiogenic factors production and acts as an antioxidant. Moreover, reduction in nitric oxide (NO) bioavailability is related to hypertensive pregnancy and H S may interact with NO, modulating its production. We aimed to investigate the NaHS effects in hypertension-in-pregnancy and also in feto-placental parameters. Female Wistar rats (200-250 g) were mated and desoxycorticosterone acetate injections followed by replacement of water by 0.9% saline solution were used to induce hypertensive pregnancy. Rats were divided into four groups: normal pregnant (Norm-Preg), pregnant + NaHS (Preg+NaHS), hypertensive pregnant (HTN-Preg) and HTN-Preg+NaHS. Systolic blood pressure was increased in HTN-Preg and this increase was blunted in HTN-Preg+NaHS. Fetal and placental weights were decreased in HTN-Preg animals, while fetal growth restriction was improved in HTN-Preg+NaHS. Placental weight was lower in HTN-Preg+NaHS than in HTN-Preg; however, placental efficiency was re-established in HTN-Preg+NaHS rats. We observed that a partial contribution of placental NO, but not changes in anti-angiogenic factors may mediate the increases in placental efficiency in HTN-Preg+NaHS. HTN-Preg presented thoracic aorta hyperreactivity to phenylephrine while NaHS treatment blunted this hyperreactivity, which seems not to be related to NO-mediated relaxation induced by acetylcholine. Therefore, changes in vascular responsiveness promoted by NaHS treatment may underlie the beneficial effects in systolic blood pressure and feto-placental parameters in our study.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Increases in placental nitric oxide, but not nitric oxide‐mediated relaxation, underlie the improvement in placental efficiency and antihypertensive effects of hydrogen sulphide donor in hypertensive pregnancy

Possomato-Vieira

Chimini

Silva

et al. 2018

Clin Exp Pharma Physio

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“…This indicates that the physical signal can induce more signaling pathways in cellular responses when compared with the chemical signal. The IPA analysis showed that these co-regulated proteins can promote the production of nitric oxide, heat shock response, STAT3 and ERK signaling [7,17].…”

Section: Discussionmentioning

confidence: 99%

“…ECs exposed to laminar SF prevent the progression of atherosclerosis [6]. Shear force can induce the production of hydrogen sulfide (H2S) that can activate the nitric oxide (NO) signaling cascade in endothelial cells [7]. Laminar SF for at least 4 hours can activate SIRT1 and promotes autophagy by removing ROS, which is thought to be important in maintaining endothelial mitochondrial function [8,9].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the protein acetylome of endothelial cells upon shear flow and resveratrol treatment

Lin

Liu

et al. 2020

Cardiol J.

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Background: Posttranslational acetylation/deacetylation known as the acetylome is important in regulating protein activity. Shear flow (SF) and resveratrol (RSV) are two stimuli that represent physical and chemical signals separately. The acetylome co-regulated by these two stimuli remain unclear. Methods: Human umbilical cord vein endothelial cells (HUVECs) were subjected to either SF of 12 dynes/cm 2 or 10 μM RSV. The purified acetylated peptides were labeled by isobaric tags for relative and absolute quantitation (iTRAQ) analysis. The signaling cascades of the identified acetylome were predicted by ingenuity pathway analysis (IPA). Coimmunoprecipitation was applied to confirm the acetylation status of the proteins. Results: Five groups of proteins showed an increased acetylation upon SF and RSV treatment. After algorithm, 628 proteins with increased acetylation and 22 proteins with decreased acetylation were identified in the SF acetylome. For the acetylome regulated by RSV, 145 proteins with increased acetylation and 23 proteins with decreased acetylation were identified. These two acetylomes were compared, and 129 proteins with increased acetylation and 2 proteins with decreased acetylation were co-regulated by both SF and RSV treatments. IPA analysis showed that this co-regulated acetylome was involved in a heat shock response, and the signals of eNOS, STAT3, JAK/STAT and ERK/MAPK. Co-immunoprecipitation analysis further confirmed the acetylated status of mitochondrial HSP60 and mitochondrial citrate synthase. Conclusions: This study indicated that a physical signal is more complicated than a chemical signal in the case of acetylome. The co-regulated proteins are worthy of further study in discussion of the synergetic effect between a physical and a chemical signal in cardioprotection.

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“…However, when relaxation was triggered by ACh in the presence of NOS inhibitor L-NAME, a relaxation around 40% was reached in aortic rings from animals of Pb + NaHS group. Previous studies showed that H 2 S may increase expression and activity of eNOS [ 77 , 78 ], thus producing more NO, and this excessive NO could be responsible for relaxation observed in the presence of L-NAME. However, we did not observe any alteration in plasmatic total NO x from animals of different groups; and interesting data was observed with nitrite measured in HUVECs' culture supernatant.…”

Section: Discussionmentioning

confidence: 99%

Clinical and Experimental Evidences of Hydrogen Sulfide Involvement in Lead-Induced Hypertension

Possomato-Vieira

Gonçalves-Rizzi

Nascimento

et al. 2018

BioMed Research International

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Lead- (Pb-) induced hypertension has been shown in humans and experimental animals and cardiovascular effects of hydrogen sulfide (H2S) have been reported previously. However, no studies examined involvement of H2S in Pb-induced hypertension. We found increases in diastolic blood pressure and mean blood pressure in Pb-intoxicated humans followed by diminished H2S plasmatic levels. In order to expand our findings, male Wistar rats were divided into four groups: Saline, Pb, NaHS, and Pb + NaHS. Pb-intoxicated animals received intraperitoneally (i.p.) 1st dose of 8 μg/100 g of Pb acetate and subsequent doses of 0.1 μg/100 g for seven days and sodium hydrosulfide- (NaHS-) treated animals received i.p. NaHS injections (50 μmol/kg/twice daily) for seven days. NaHS treatment blunted increases in systolic blood pressure, increased H2S plasmatic levels, and diminished whole-blood lead levels. Treatment with NaHS in Pb-induced hypertension seems to induce a protective role in rat aorta which is dependent on endothelium and seems to promote non-NO-mediated relaxation. Pb-intoxication increased oxidative stress in rats, while treatment with NaHS blunted increases in plasmatic MDA levels and increased antioxidant status of plasma. Therefore, H2S pathway may be involved in Pb-induced hypertension and treatment with NaHS exerts antihypertensive effect, promotes non-NO-mediated relaxation, and decreases oxidative stress in rats with Pb-induced hypertension.

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Laminar shear flow increases hydrogen sulfide and activates a nitric oxide producing signaling cascade in endothelial cells

Cited by 27 publications

References 35 publications

Increases in placental nitric oxide, but not nitric oxide‐mediated relaxation, underlie the improvement in placental efficiency and antihypertensive effects of hydrogen sulphide donor in hypertensive pregnancy

Increases in placental nitric oxide, but not nitric oxide‐mediated relaxation, underlie the improvement in placental efficiency and antihypertensive effects of hydrogen sulphide donor in hypertensive pregnancy

Comparison of the protein acetylome of endothelial cells upon shear flow and resveratrol treatment

Clinical and Experimental Evidences of Hydrogen Sulfide Involvement in Lead-Induced Hypertension

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