Laminar shear, but not orbital shear, has a synergistic effect with thrombin stimulation on tissue factor expression in human umbilical vein endothelial cells

Rochier, Adrienne; Nixon, Alexander; Yamashita, Norio; Abe, Ryuzo; Abe, Rei; Madri, Joseph A.; Sumpio, Bauer E.

doi:10.1016/j.jvs.2011.01.002

Cited by 11 publications

(12 citation statements)

References 38 publications

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“…In particular, endothelial cells could experience shear exerted by the flow of blood, causing them to become aligned and elongated with the direction of flow and to undergo other biochemical changes [2, 41]. More importantly, orbital shakers provide oscillatory flow, somewhat like the pulsing fluid movement in the human vasculature system [41–43].…”

Section: Resultsmentioning

confidence: 99%

Synergistic Effects of Orbital Shear Stress onIn VitroGrowth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

Lim¹,

Hexiu

Kim

et al. 2014

BioMed Research International

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Cellular behavior is dependent on a variety of physical cues required for normal tissue function. In order to mimic native tissue environments, human alveolar bone-derived mesenchymal stem cells (hABMSCs) were exposed to orbital shear stress (OSS) in a low-speed orbital shaker. The synergistic effects of OSS on proliferation and differentiation of hABMSCs were investigated. In particular, we induced the osteoblastic differentiation of hABMSCs cultured in the absence of OM by exposing hABMSCs to OSS (0.86–1.51 dyne/cm2). Activation of Cx43 was associated with exposure of hABMSCs to OSS. The viability of cells stimulated for 10, 30, 60, 120, and 180 min/day increased by approximately 10% compared with that of control. The OSS groups with stimulation of 10, 30, and 60 min/day had more intense mineralized nodules compared with the control group. In quantification of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2) protein, VEGF protein levels under stimulation for 10, 60, and 180 min/day and BMP-2 levels under stimulation for 60, 120, and 180 min/day were significantly different compared with those of the control. In conclusion, the results indicated that exposing hABMSCs to OSS enhanced their differentiation and maturation.

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Section: Resultsmentioning

confidence: 99%

Synergistic Effects of Orbital Shear Stress onIn VitroGrowth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

Lim¹,

Hexiu

Kim

et al. 2014

BioMed Research International

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“…As discussed above, steady laminar shear stress is a determinant of normal vascular function through orchestration of activities of eNOS/COX-2 and consequently NO/PGI 2 (two critical molecules for vasodilatations and anti-platelet aggregation). Steady wall shear stress is also important to downregulate pro-thrombotic molecules, such as tissue factor, an initiator of thrombus formation [46]. The major fibrinolytic molecule, tissue plasminogen activator (tPA) is also regulated by shear stress.…”

Section: Endothelium Signalling Pathway Under Shear Stressmentioning

confidence: 99%

Role of shear stress and stretch in vascular mechanobiology

Kassab

2011

J. R. Soc. Interface.

270

198

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Blood vessels are under constant mechanical loading from blood pressure and flow which cause internal stresses (endothelial shear stress and circumferential wall stress, respectively). The mechanical forces not only cause morphological changes of endothelium and blood vessel wall, but also trigger biochemical and biological events. There is considerable evidence that physiologic stresses and strains (stretch) exert vasoprotective roles via nitric oxide and provide a homeostatic oxidative balance. A perturbation of tissue stresses and strains can disturb biochemical homeostasis and lead to vascular remodelling and possible dysfunction (e.g. altered vasorelaxation, tone, stiffness, etc.). These distinct biological endpoints are caused by some common biochemical pathways. The focus of this brief review is to point out some possible commonalities in the molecular pathways in response to endothelial shear stress and circumferential wall stretch.

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“…We have previously reported the time course of TF expression of ECs exposed to laminar flow and this started by 2 hr, peaked at 4 hrs and then started to decline by 8 hrs. [7,11,12]. All mechanical stimuli conditions induced higher TF RNA expression compared with static conditions (ANOVA and posthoc analysis of Bonferroni, P<0.05).…”

Section: Discussionmentioning

confidence: 88%

“…This dose of thrombin results in reproducible stimulation of TF expression [7,[11][12][13][14]. These experiments applying mechanical and chemical stress together were performed simultaneously with the experiments applying only mechanical stress, as paired experiments.…”

Section: Mechanical Stress Exposurementioning

confidence: 99%

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Effect of Frequency of Uniform and Disturbed Pulsatility Flow on Tissue Factor Expression of Human Umbilical Vein Endothelial Cells

Moriguchi¹,

Davis²

2013

J Vasc Med Surg

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We analyzed the effect of change in frequency of uniform or disturbed pulsatile flow on human umbilical vein endothelial cells (HUVECs) in the presence or absence of thrombin (Th). We found that tissue factor (TF) RNA expression in HUVECs exposed to 60 cycles per minute (cpm) of disturbed and uniform flow was significantly higher than that induced by 30 cpm. HUVECs exposed to disturbed flow have significantly higher TF RNA expression than HUVECs exposed to uniform flow at either frequency. We conclude that frequency of pulsatile flow is a critical independent factor in TF RNA expression in HUVECs.

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Laminar shear, but not orbital shear, has a synergistic effect with thrombin stimulation on tissue factor expression in human umbilical vein endothelial cells

Cited by 11 publications

References 38 publications

Synergistic Effects of Orbital Shear Stress onIn VitroGrowth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

Synergistic Effects of Orbital Shear Stress onIn VitroGrowth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

Role of shear stress and stretch in vascular mechanobiology

Effect of Frequency of Uniform and Disturbed Pulsatility Flow on Tissue Factor Expression of Human Umbilical Vein Endothelial Cells

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