Laminar distribution and arbor density of two functional classes of thalamic inputs to primary visual cortex

Zhuang, Jun; Wang, Yun; Ouellette, Naveen; Turschak, Emily; Larsen, Rylan S.; Takasaki, Kevin T.; Daigle, Tanya L.; Tasic, Bosiljka; Waters, Jack; Zeng, Hongkui; Reid, R. Clay

doi:10.1016/j.celrep.2021.109826

Cited by 8 publications

(28 citation statements)

References 43 publications

(70 reference statements)

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“…Our previous study has shown the low orientation selectivity in the LGN axons projecting to the layer 4 of V1 ( Kondo and Ohki, 2016 ), which is different from the results of other studies ( Sun et al, 2016 ; Zhuang et al, 2021 ). The reason for the discrepancy between these studies could be the differences in experimental conditions, particularly, the type of promoter used to express the genetically encoded calcium indicator protein, GCaMP6s.…”

Section: Discussioncontrasting

confidence: 94%

“…One study has shown that the projecting axons in the layer 4 have lower orientation selectivity than the projecting axons in the layer 1 ( Kondo and Ohki, 2016 ). However, other studies have reported different results that thalamic axons projecting to both the layers 1 and 4 have high orientation selectivity ( Sun et al, 2016 ; Zhuang et al, 2021 ). To determine the orientation selectivity of LPN axons projecting to the different layers of V1 and compare their orientation selectivity with that of LGN axons, in vivo two-photon calcium imaging of LPN axons was conducted using GCaMP6s.…”

Section: Resultsmentioning

confidence: 85%

“…Previous studies have shown the orientation selectivity of LGN axons distributed in V1 ( Kondo and Ohki, 2016 ; Sun et al, 2016 ; Zhuang et al, 2021 ). One study has shown that the projecting axons in the layer 4 have lower orientation selectivity than the projecting axons in the layer 1 ( Kondo and Ohki, 2016 ).…”

Section: Resultsmentioning

confidence: 96%

“…Another difference between our study and the two studies is the optics of two-photon microscope. Sun et al (2016) and Zhuang et al (2021) used adaptive optics (AO) to improve the distortion of the fluorescence signal due to optical aberrations in the recordings from the deep cortical layers. A previous study showed that the sample-induced aberration during the in vivo brain imaging is mostly a spherical aberration ( Ji et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…Since our recordings were done by this method, the effect of sample-aberration was minimized without AO. Indeed, Zhuang et al (2021) tested this possibility and found that AO improves the signal intensity of visual responses, but does not affect the calculation of the orientation selectivity of axonal boutons in layer 4. In the present study, we found that the LPN axons projecting to the layer 4 of V1 have high orientation selectivity, suggesting that our correction method is enough to see the orientation selectivity, and the low orientation selectivity of the LGN axons projecting to V1 ( Kondo and Ohki, 2016 ) was not due to the sample aberration.…”

Section: Discussionmentioning

confidence: 99%

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Response Selectivity of the Lateral Posterior Nucleus Axons Projecting to the Mouse Primary Visual Cortex

Kondo

Kiyohara

Ohki

2022

Front. Neural Circuits

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Neurons in the mouse primary visual cortex (V1) exhibit characteristic response selectivity to visual stimuli, such as orientation, direction and spatial frequency selectivity. Since V1 receives thalamic visual inputs from the lateral geniculate nucleus (LGN) and lateral posterior nucleus (LPN), the response selectivity of the V1 neurons could be influenced mostly by these inputs. However, it remains unclear how these two thalamic inputs contribute to the response selectivity of the V1 neurons. In this study, we examined the orientation, direction and spatial frequency selectivity of the LPN axons projecting to V1 and compared their response selectivity with our previous results of the LGN axons in mice. For this purpose, the genetically encoded calcium indicator, GCaMP6s, was locally expressed in the LPN using the adeno-associated virus (AAV) infection method. Visual stimulations were presented, and axonal imaging was conducted in V1 by two-photon calcium imaging in vivo. We found that LPN axons primarily terminate in layers 1 and 5 and, to a lesser extent, in layers 2/3 and 4 of V1, while LGN axons mainly terminate in layer 4 and, to a lesser extent, in layers 1 and 2/3 of V1. LPN axons send highly orientation- and direction-selective inputs to all the examined layers in V1, whereas LGN axons send highly orientation- and direction-selective inputs to layers 1 and 2/3 but low orientation and direction selective inputs to layer 4 in V1. The distribution of preferred orientation and direction was strongly biased toward specific orientations and directions in LPN axons, while weakly biased to cardinal orientations and directions in LGN axons. In spatial frequency tuning, both the LPN and LGN axons send selective inputs to V1. The distribution of preferred spatial frequency was more diverse in the LPN axons than in the LGN axons. In conclusion, LPN inputs to V1 are functionally different from LGN inputs and may have different roles in the orientation, direction and spatial frequency tuning of the V1 neurons.

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Section: Discussioncontrasting

confidence: 94%