Laminar and dorsoventral organization of layer 1 interneuronal microcircuitry in superficial layers of the medial entorhinal cortex

Shi, Yanting; Cui, Han; Li, Xiaoyue; Chen, L.; Zhang, Chen; Zhao, Xinran; Li, Xiaowan; Shao, Qianjun; Sun, Qiang; Yan, Kaiming; Wang, Guangfu

doi:10.1016/j.celrep.2023.112782

Cited by 9 publications

(5 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Third, longer range output from SCs to non-PV INs may support coordination between modules. Candidates for this coordination could include interneurons on L1 of the MEC, which can have extensive intra-laminar axons (Shi et al, 2023), or PV negative interneurons in layer 2. We suggest this model as a starting point for further investigation of MEC connectivity.…”

Section: Discussionmentioning

confidence: 99%

Synaptic interactions between stellate cells and parvalbumin interneurons in layer 2 of the medial entorhinal cortex are organized at the scale of grid cell clusters

Huang,

Garden,

McCLure

et al. 2023

Preprint

View full text Add to dashboard Cite

Interactions between excitatory and inhibitory neurons are critical to computations in cortical circuits but their organization is difficult to assess with standard electrophysiological approaches. Within the medial entorhinal cortex, representation of location by grid and other spatial cells involves circuits in layer 2 in which excitatory stellate cells interact with each other via inhibitory parvalbumin expressing interneurons. Whether this connectivity is structured to support local circuit computations is unclear. Here, we introduce strategies to address the functional organization of excitatory-inhibitory interactions using crossed Cre- and Flp-driver lines to direct targeted presynaptic optogenetic activation and postsynaptic cell identification. We then use simultaneous patch-clamp recordings from postsynaptic neurons to assess their shared input from optically activated presynaptic populations. We find that extensive axonal projections support dense but incomplete connectivity between stellate cells and parvalbumin interneurons. Analysis of the spatial organization of synaptic interactions is consistent with local modules defined by excitatory-inhibitory connectivity. We suggest that dense, but specific, direct excitatory-inhibitory synaptic interactions may operate at the scale of grid cell clusters, while indirect interactions may coordinate activity at the scale of grid cell modules.

show abstract

Section: Discussionmentioning

confidence: 99%

Synaptic interactions between stellate cells and parvalbumin interneurons in layer 2 of the medial entorhinal cortex are organized at the scale of grid cell clusters

Huang,

Garden,

McCLure

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Reconstructed neurons are adapted with permission from Shi et al. 1 Transfer the slices using a pipette and mount them on a glass slide. Use a micropipette and filter paper to remove excess 0.1 M PB solution and permit the slices to dry in air for a while, and then seal the slices with Mowiol solution.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…This protocol was fully executed in the study by Shi et al., 1 where young adult male and female mice (≥P22, with ∼90% of them to be P25-65), including wild type or Vgat-Cre/Ai9, PV-Cre/Ai9, SOM-Cre/Ai9 and VIP-Cre/Ai9 mice, were used. The Cre and Ai9 reporter lines were purchased from Jackson Laboratory (Vgat-Cre, #028862; PV-Cre, #017320; SOM-Cre, #013044; VIP-Cre, #010908; Ai9, #007909).…”

Section: Before You Beginmentioning

confidence: 99%

“…This protocol is specifically designed for simultaneous multiple patch-clamp recordings and morphological reconstruction of MEC neurons, as validated in our previous study. 1 However, with slight modification, it should also be used in research of other brain areas like the neocortex. 3 , 4 With the current protocol, it is expected to obtain sub- and supra-threshold electrophysiological properties of diverse MEC neurons and evaluate uEPSP/uIPSP and electrical coupling between these neurons.…”

Section: Expected Outcomesmentioning

confidence: 99%

“…With these data, different cell types of neurons can be identified based on their morphological and electrophysiological features and cell-type-specific chemical and electrical microcircuits can be demonstrated. 1 …”

Section: Expected Outcomesmentioning

confidence: 99%

See 2 more Smart Citations

Protocol to study microcircuits in the medial entorhinal cortex in mice using multiple patch-clamp recordings and morphological reconstruction

Shi,

Wang

2024

STAR Protocols

Self Cite

View full text Add to dashboard Cite

Architectural organization of ∼1,500-neuron modular minicolumnar disinhibitory circuits in healthy and Alzheimer’s cortices

Zhu

2023

Cell Reports

View full text Add to dashboard Cite

Laminar and dorsoventral organization of layer 1 interneuronal microcircuitry in superficial layers of the medial entorhinal cortex

Cited by 9 publications

References 86 publications

Synaptic interactions between stellate cells and parvalbumin interneurons in layer 2 of the medial entorhinal cortex are organized at the scale of grid cell clusters

Synaptic interactions between stellate cells and parvalbumin interneurons in layer 2 of the medial entorhinal cortex are organized at the scale of grid cell clusters

Protocol to study microcircuits in the medial entorhinal cortex in mice using multiple patch-clamp recordings and morphological reconstruction

Architectural organization of ∼1,500-neuron modular minicolumnar disinhibitory circuits in healthy and Alzheimer’s cortices

Contact Info

Product

Resources

About