Lamina Propria Phagocyte Profiling Reveals Targetable Signaling Pathways in Refractory Inflammatory Bowel Disease

Jacobsen, Gillian E.; Fernández, Irina; Quintero, Maria A.; Santander, Ana M.; Pignac-Kobinger, Judith; Damas, Oriana M.; Deshpande, Amar R.; Kerman, David H.; Ban, Yuguang; Silva, Tiago C.; Wang, Lily; Beecham, Ashley; McCauley, Jacob L.; Burgueño, Juan F.; Abreu, María T.

doi:10.1016/j.gastha.2022.01.005

Cited by 7 publications

(2 citation statements)

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“…However, many studies have clarified that the production of proinflammatory cytokines in the mucosal immune system plays a crucial role in IBD. 5 More importantly, a variety of cytokines are secreted by a large number of macrophages of IBD patients, such as interleukin IL‐1β, IL‐6, IL‐18, and TNF‐α. All of these factors enhance the intensity of the inflammatory response and increase the severity of colitis.…”

Section: Introductionmentioning

confidence: 99%

“…Current studies cannot elucidate the exact pathogenesis of IBD. However, many studies have clarified that the production of proinflammatory cytokines in the mucosal immune system plays a crucial role in IBD 5 . More importantly, a variety of cytokines are secreted by a large number of macrophages of IBD patients, such as interleukin IL‐1β, IL‐6, IL‐18, and TNF‐α.…”

Section: Introductionmentioning

confidence: 99%

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Ononin alleviates DSS‐induced colitis through inhibiting NLRP3 inflammasome via triggering mitophagy

Liang

et al. 2023

Immunity Inflam & Disease

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Background: Ononin, a flavonoid isolated from Astragalus membranaceus root, is the active ingredient of A. membranaceus and has potential anti‐inflammatory properties, but its effect on colitis is unclear. Aims: This study aimed to explore the anticolitis effect of Ononin by establishing a colitis model in mice induced by dextran sulfate sodium (DSS). Methods: Male C57BL/6 mice were provided DSS, then treated with Ononin (10, 20, 40 mg/kg) or 5‐ASA (40 mg/kg). The colitis symptoms were observed, the disease activity index (DAI) score were recorded daily, and colonic inflammation was evaluted by histopathological scoring. The expression of cytokines, inflammatory mediators, and mitophagy/NLRP3 inflammasome‐related proteins were measured. Results: Ononin significantly alleviated weight loss and colon shortening in mice with colitis ( p < .01). Moreover, Ononin decreased the production of inflammatory cytokines and mediators associated with colitis ( p < .05). In addition, Ononin inhibited macrophages infiltration and reduced caspase‐1 activation in colitis mice. Caspase‐1 activation is closely related to the NLRP3 inflammasome. Therefore, we investigated the effect of Ononin on NLRP3 inflammasome in vitro. The relevant results confirmed that Ononin inhibited NLRP3 inflammasome activation and inhibited mitochondrial damage ( p < .05). Further studies revealed that Ononin inhibited mitochondrial damage through triggering mitophagy ( p < .05). Conclusion: Ononin alleviates DSS‐induced colitis by activating mitophagy to inhibit NLRP3 inflammasome.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%