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The homogeneous polysaccharide S b RP-1cc with MW 110 kDa was isolated during an investigation of watersoluble polysaccharides from Scutellaria baicalensis roots. Its aqueous solution gave a positive reaction with iodine and exhibited high specific rotation ([D] D +188.0°). Chemical, chromatographic, and spectral methods established that S b RP-1s was a slightly branched glucan containing in the main chain D-(1o4)-glucopyranose units, 8.3% of which were branched at the C-6 atom with single D-glucopyranose units. Glucan S b RP-1s was shown to exhibit immunostimulating activity using an azathioprine model of immunosuppression.We have continued our study of the carbohydrate components of plants from the family Lamiaceae [1]. Results from a study of the water-soluble polysaccharides (WSPS) from the aerial part of Scutellaria baicalensis Georgi were reported earlier [2]. Information on the polysaccharides from roots of this species has not been reported. The goal of the present work was to isolate and study the structure and biological activity of the dominant component of the WSPS complex from S. baicalensis roots.WSPS were isolated from S. baicalensis roots using hot water extraction of raw material treated beforehand with EtOH (80%). This produced the fraction of total WSPS S b RP in 2.53% yield of the air-dried raw material mass. Solutions of S b RP gave a positive reaction with iodine and showed high specific rotation ([D] D +190.4°). Hydrolysates of S b RP contained glucose, galacturonic acid, galactose, arabinose, and mannose in a 33.5:3.0:2.4:1.8:1 ratio. Gel chromatography showed the S b RP was heterogeneous, because of which it was fractionated using ion-exchange (DEAE-cellulose) and gel-permeation chromatography (Sephacryl 300-HR, Sephadex G-200). This isolated the homogeneous component S b RP-1s with molecular weight 110 kDa that eluted as a single symmetric peak by chromatography over Sephadex G-200. The studies showed that S b RP-1s contained 97.2% carbohydrates; uronic acids and proteins were not observed. Aqueous solutions of the polymer reacted with iodine solution (O max 615 nm) and gave positive specific rotation ([D] D +188.0°). Chromatography (HPTLC, GC/MS) of the hydrolysate of S b RP-1s detected only glucose. The IR spectrum of the polymer showed bands assigned to absorption by an D-bond (852 cm -1 ) and bands characteristic of D-(1o4)-glucans (914, 1024, 1050, 1154 cm -1 ) [3]. Oxidation of S b RP-1s acetate by chromic anhydride and hydrolysis of the oxidation product established the presence of glucose, which was possible for the D-configuration of the glucose units. Periodate oxidation of the polymer consumed 1.08 mol IO 4 -and released 0.08 mol HCOOH per single anhydro unit. Smith degradation produced glycerin and erythrite in a 1:12.06 ratio, which indicated the presence of (1o4)-and (1o6)-bonds. The completely methylated polysaccharide was hydrolyzed. The decomposition products were analyzed as the aldoacetates by GC/MS. Three types of glucose derivatives were found. These were 1,5-diace...
The homogeneous polysaccharide S b RP-1cc with MW 110 kDa was isolated during an investigation of watersoluble polysaccharides from Scutellaria baicalensis roots. Its aqueous solution gave a positive reaction with iodine and exhibited high specific rotation ([D] D +188.0°). Chemical, chromatographic, and spectral methods established that S b RP-1s was a slightly branched glucan containing in the main chain D-(1o4)-glucopyranose units, 8.3% of which were branched at the C-6 atom with single D-glucopyranose units. Glucan S b RP-1s was shown to exhibit immunostimulating activity using an azathioprine model of immunosuppression.We have continued our study of the carbohydrate components of plants from the family Lamiaceae [1]. Results from a study of the water-soluble polysaccharides (WSPS) from the aerial part of Scutellaria baicalensis Georgi were reported earlier [2]. Information on the polysaccharides from roots of this species has not been reported. The goal of the present work was to isolate and study the structure and biological activity of the dominant component of the WSPS complex from S. baicalensis roots.WSPS were isolated from S. baicalensis roots using hot water extraction of raw material treated beforehand with EtOH (80%). This produced the fraction of total WSPS S b RP in 2.53% yield of the air-dried raw material mass. Solutions of S b RP gave a positive reaction with iodine and showed high specific rotation ([D] D +190.4°). Hydrolysates of S b RP contained glucose, galacturonic acid, galactose, arabinose, and mannose in a 33.5:3.0:2.4:1.8:1 ratio. Gel chromatography showed the S b RP was heterogeneous, because of which it was fractionated using ion-exchange (DEAE-cellulose) and gel-permeation chromatography (Sephacryl 300-HR, Sephadex G-200). This isolated the homogeneous component S b RP-1s with molecular weight 110 kDa that eluted as a single symmetric peak by chromatography over Sephadex G-200. The studies showed that S b RP-1s contained 97.2% carbohydrates; uronic acids and proteins were not observed. Aqueous solutions of the polymer reacted with iodine solution (O max 615 nm) and gave positive specific rotation ([D] D +188.0°). Chromatography (HPTLC, GC/MS) of the hydrolysate of S b RP-1s detected only glucose. The IR spectrum of the polymer showed bands assigned to absorption by an D-bond (852 cm -1 ) and bands characteristic of D-(1o4)-glucans (914, 1024, 1050, 1154 cm -1 ) [3]. Oxidation of S b RP-1s acetate by chromic anhydride and hydrolysis of the oxidation product established the presence of glucose, which was possible for the D-configuration of the glucose units. Periodate oxidation of the polymer consumed 1.08 mol IO 4 -and released 0.08 mol HCOOH per single anhydro unit. Smith degradation produced glycerin and erythrite in a 1:12.06 ratio, which indicated the presence of (1o4)-and (1o6)-bonds. The completely methylated polysaccharide was hydrolyzed. The decomposition products were analyzed as the aldoacetates by GC/MS. Three types of glucose derivatives were found. These were 1,5-diace...
Introduction. The search, development and introduction of new drugs with an immunotropic effect are one of the priority tasks of modern immunopharmacology. Numerous studies have proven the immunotropic activity of individual substances isolated from medicinal plants (flavonoids, polysaccharides, ecdysteroids, terpenoids, etc.). In the present study, it is of interest to determine the immunomodulatory effect of individual substances isolated from Silene jeniseensis Willd.The aim of the study. Determination of the immunomodulatory activity of individual substances isolated from Silene jeniseensis: flavonoid isoorientin-2”-O-rhamnoside, polysaccharide arabino-3.6-galactan and ecdysteroid 20-hydroxyecdysone under conditions of cyclophosphamide induced experimental immunosuppression.Methods. Experiments were carried out on F1 (CBAxC57Bl/6) mice. Immunodeficiency was modeled by intraperitoneal administration of cyclophosphamide to control group animals in the dose 250 mg/kg once. Experimental groups of mice received the test substances intragastrically once a day for 14 days against the background of immunosuppression in the following doses: isoorientin-2”-O-rhamnoside – 10 mg/ kg, arabino-3.6-galactan – 3 mg/kg, 20-hydroxyecdysone – 3 mg/kg. The effect of substances on cellular immunity was determined in a delayed hypersensitivity reaction, humoral immunity was determined in an antibody formation reaction by local hemolysis according to A.J. Cunningham. The phagocytic activity of peritoneal macrophages was studied in relation to colloidal ink particles.Results. With the introduction of isoorientin-2”-O-rhamnoside, arabino-3.6-galactan and 20-hydroxyecdysone in experimental animals, there was an increase in the index of delayed-type hypersensitivity reaction by 1.3–1.4 times, the absolute and relative number of antibody-forming cells by 1.4–1.7 times, phagocytic index by 1.2–1.5 times compared with the data in the control group, which indicates the leveling of the suppressive effect of cyclophosphamide on cell-mediated immune response, antibody genesis and phagocytosis of macrophages.Conclusion. Isoorientin-2”-O-rhamnoside and arabino-3.6-galactan have the most pronounced immunomodulatory effect. The obtained data allow us to consider the studied substances as promising plant immunomodulators.
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