Lambing rates and litter sizes following intrauterine or cervical insemination of frozen/thawed semen with or without oxytocin administration

King, Margaret E; McKelvey, W.A.C.; Dingwall, W.S.; Matthews, Katy; Gebbie, F.E.; Mylne, M. J. A.; Stewart, Elizabeth A.; Robinson, John J.

doi:10.1016/j.theriogenology.2004.01.009

Cited by 68 publications

(58 citation statements)

References 18 publications

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“…In the present study, the administration of oxytocin at the time of AI decreased CR in lactating dairy cows. This could be due to changes in uterine contractility and possibly to the impairment of sperm transport in the reproductive tract of cows in contrast with other species (Hawk 1983;Sayre and Lewis, 1997;King et al, 2004).…”

Section: Discussionmentioning

confidence: 98%

“…Oxytocin was used to increase conception rate (CR) by improving the sperm transport in the female reproductive tract of several species (Hays et al, 1958;Hawk 1987;Sayre and Lewis 1997;King et al, 2004;Yildiz, 2005). Clitoral massage which probably releases oxytocin following artificial insemination increased pregnancy in beef cows but not in beef heifers or dairy cows (Randel et al, 1975;Cooper et al, 1984).…”

Section: Effects Of Gnrhmentioning

confidence: 99%

See 1 more Smart Citation

Effects of GnRH, PGF<sub>2α</sub> and oxytocin treatments on conception rate at the time of artificial insemination in lactating dairy cows

Gümen¹,

Keskin²,

Yılmazbaş-Meci̇toğlu³

et al. 2011

CZECH J ANIM SCI

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AbstRAct:In several studies, hormones such as gonadotropin-releasing hormone (GnRH), prostaglandins and oxytocin were used to increase pregnancy rate by inducing ovulation and improving the sperm transport in the female reproductive tract in lactating dairy cattle. The objective of this study was to compare the effects of GnRH, prostaglandin F 2α (PGF 2α ) and oxytocin treatments at the time of artificial insemination (AI) after spontaneous oestrus on the conception rate (CR) of lactating dairy cows. Oestrus was detected by visual observations by experienced personnel. All cows (n = 430, 308 Holstein-Frisian and 122 SwedishRed dairy cows) were inseminated based on the am/pm rule by veterinarians of the farm. After AI, cows were alternately assigned to one of the four treatment groups: (1) GnRH (n = 113); (2) PGF 2α (n = 106); (3) oxytocin (n = 106) and (4) non-treated control (n = 105). Pregnancy diagnosis was performed 28-34 and 58-64 days post-insemination by transrectal ultrasonography. Conception rates on days 28-34 and 58-64 were not different among GnRH (46.0%; 52/113 and 44.3%; 50/113), PGF 2α (37.7%; 40/106 and 35.9%; 38/106) and control (49.5%; 52/105 and 47.6%; 50/105) groups. However, conception rates were lower (P = 0.02) in oxytocin (31.1%; 33/106 and 30.2%; 32/106) than in GnRH and control groups on days 28-34 and 58-64. Other covariant factors, such as milk production, days in milk (DIM), breed, parity, service number did not affect the conception rate. Thus, there were no beneficial effects of treatments with GnRH and PGF 2α at the time of AI, and oxytocin had an adverse effect on CR in lactating dairy cows in this study.

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Section: Discussionmentioning

confidence: 98%

Section: Effects Of Gnrhmentioning

confidence: 99%

Effects of GnRH, PGF<sub>2α</sub> and oxytocin treatments on conception rate at the time of artificial insemination in lactating dairy cows

Gümen¹,

Keskin²,

Yılmazbaş-Meci̇toğlu³

et al. 2011

CZECH J ANIM SCI

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“…However, it is also the most expensive and complicated method. Apart from the general trends, the publications show quite a wide range of rates: 31.25% (Anel et al, 2005) in vaginal, 18 (Yamaki et al, 2003); 42 (King et al, 2004); and 65-75% (Salamon & Maxwell, 1995;Donovan et al, 2001Donovan et al, , 2004Stefanov et al, 2006) in cervical; 69.6 to 76.4% (Szabados, 2006) in cevico-uterinal;and 44.89 (Anel et al, 2005), 64 (Yamaki et al, 2003), and 69 % (King et al, 2004) in laparoscopic insemination. It should be noted that these results are affected according to the semen used (fresh, cooled, chilled or frozen (Perkins et al, 1996;Moses et al, 1997).…”

Section: The Methods (Vaginal Cervical Cervico-uterinal or Laparoscomentioning

confidence: 99%

“…About 50 to 100 million spermatozoa is needed in one dose to vaginal insemination, but 25 to 50 million are suggested for cervical insemination and 15 to 20 million is enough for laparoscopic AI (Kukovics, 1974;Jávor et al, 2006;Gergátz, 2007). However, several scientists have used much higher numbers: 106 (Fernandez-Abella et al, 2003), 50 to 300 (Ehling et al, 2003) and 400 (King et al, 2004) million spermatozoa in one dose of semen.…”

Section: Dose Of Inseminated Semenmentioning

confidence: 99%

Artificial Insemination of Sheep - Possibilities, Realities and Techniques at the Farm Level

Kukovics¹,

Gyoker²,

Németh³

et al. 2011

Artificial Insemination in Farm Animals

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“…The most important element in AI is the preservation of ram semen through liquid storage or freezing (Evans and Maxwell 1987). Freezing ram semen generally results in poor pregnancy rates in cervical insemination, compared to pregnancy rates with fresh semen when AI is performed within a short time after collection (King et al 2004;Camara et al 2011). The success of this protocol in the sheep industry is related to the ram semen quality.…”

mentioning

confidence: 99%

Positive effects of trehalose and cysteine on ram sperm parameters

Güngör¹,

Öztürk²,

Omur³

2017

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to determine the effects of trehalose and cysteine on sperm motility, viability, mitochondrial activity and acrosome integrity during liquid storage of Merino ram semen. Ejaculates were collected using artificial vaginas from five Merino rams, microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including cysteine (2 mM and 4 mM), trehalose (10 mM and 25 mM) and no antioxidant (control). Diluted semen samples were kept in tubes and cooled from 37 to 5 °C in a cold cabinet, and maintained at 5 °C. Cooled samples were evaluated for sperm motility, viability, mitochondrial activity and acrosome integrity at 0, 24, 48, 72 and 96 h. Extender supplemented with trehalose (10 and 25 mM) and cysteine (2 and 4 mM) led to higher motility in comparison to the control at 24, 48, 72 and 96 h of liquid storage (P < 0.05). Trehalose at the doses of 10 mM, 25 mM and 2 mM cysteine led to higher viability between 24-48-72 h and at 96 h of liquid storage (P < 0.05). Further, 4 mM of cysteine improved sperm viability rates at 24 and 48 h of storage compared to the control group (P < 0.05), and resulted in improved acrosome integrity rates compared to the control group at 72 and 96 h of storage (P < 0.05). Extender supplemented with 10 and 25 mM trehalose at 24 and 72 h and 4 mM cysteine at 24 and 96 h of storage led to higher sperm mitochondrial activity rates when compared to the control group (P < 0.05). In conclusion, the findings of this study show that trehalose and cysteine provided significant protection to ram sperm parameters during liquid storage.

show abstract

Lambing rates and litter sizes following intrauterine or cervical insemination of frozen/thawed semen with or without oxytocin administration

Cited by 68 publications

References 18 publications

Effects of GnRH, PGF<sub>2α</sub> and oxytocin treatments on conception rate at the time of artificial insemination in lactating dairy cows

Effects of GnRH, PGF<sub>2α</sub> and oxytocin treatments on conception rate at the time of artificial insemination in lactating dairy cows

Artificial Insemination of Sheep - Possibilities, Realities and Techniques at the Farm Level

Positive effects of trehalose and cysteine on ram sperm parameters

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